研究目的
To investigate and develop a low-cost tapered plastic fiber optic biosensor based on manipulation of colloidal gold nanoparticles for enhanced sensitivity in detecting Bovine Serum Albumin.
研究成果
The study successfully demonstrates a low-cost biosensor using tapered plastic fiber and gold nanoparticles, achieving a limit of detection of 0.326 gm/dL for BSA, which is suitable for clinical albumin level monitoring. The enhancement in sensitivity is attributed to local surface plasmon resonance, and future work should focus on wavelength tuning to improve detection limits.
研究不足
The sensor's sensitivity may be limited by the fixed wavelength of the laser source (650 nm), and further optimization is needed for tuning to maximum absorption wavelengths. The coating process and nanoparticle size control could be refined for better uniformity and performance.
1：Experimental Design and Method Selection:
The study uses Finite Difference Time Domain (FDTD) simulation to model evanescent field absorption and optimize gold nanoparticle size. A biconical tapered plastic optical fiber is fabricated and coated with synthesized gold nanoparticles to enhance sensitivity through local surface plasmon resonance.
2：Sample Selection and Data Sources:
Gold nanoparticles are synthesized using a chemical reduction method with HAuCl4, NaBH4, and LEEH. Bovine Serum Albumin (BSA) solutions of varying concentrations (0.05mM, 0.1mM, 0.15mM, 0.20mM) are prepared for testing.
3：05mM, 1mM, 15mM, 20mM) are prepared for testing. List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Equipment includes a hot plate, motors (M1, M2), Arduino board, laser diode (650 nm), microscope objective (20x NA=10), photodetector, measuring unit, TEM for nanoparticle characterization. Materials include PMMA multimode fiber (250μm diameter, 0.5 NA), HAuCl4, NaBH4, LEEH, acetone, BSA from Sigma Aldrich.
4：5 NA), HAuCl4, NaBH4, LEEH, acetone, BSA from Sigma Aldrich. Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Fabricate tapered fiber by heating and pulling; synthesize gold nanoparticles by chemical reduction; coat tapered region with nanoparticles; functionalize surface; immerse in BSA solutions; measure output power using laser source and photodetector setup.
5：Data Analysis Methods:
Use FDTD simulation (Lumerical software) to analyze transmittance vs. wavelength; measure detector output to calculate limit of detection (LOD) and sensitivity; employ TEM for nanoparticle size distribution.
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FDTD Software
Lumerical FDTD
Lumerical
Commercial software used for Finite Difference Time Domain simulations to model the evanescent field absorption and optimize nanoparticle size.
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Laser Diode
650 nm
Used as a light source to launch light into the tapered fiber for sensing applications.
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Microscope Objective
20x NA=10
Maximizes coupling efficiency from the laser source to the plastic fiber.
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Photodetector
Collects transmitted light at the end of the fiber and converts it to an electrical signal for measurement.
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Measuring Unit
Measures the output from the photodetector, typically in microamperes (μA).
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Arduino Board
Arduino
Controls the motion of motors M1 and M2 during the fabrication of the tapered optical fiber.
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Hot Plate
Used to heat the aluminum block for fabricating the tapered optical fiber.
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Motors
M1, M2
Pull the optical fiber at a controlled rate (10 μm/sec) to create the taper.
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TEM
Used for Transmission Electron Microscopy to measure the size and distribution of synthesized gold nanoparticles.
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