研究目的
To synthesize nontoxic fluorescent carbon dots (C-dots) from glucose and taurine using a green hydrothermal method and investigate their application in Fe3+ and pH sensing.
研究成果
The green hydrothermal synthesis successfully produced fluorescent C-dots from glucose and taurine, with optimal conditions at 150°C, 2 hours reaction time, and 4:1 mass ratio. These C-dots exhibit pH- and Fe3+-sensitive photoluminescence, enabling applications in sensing with high sensitivity and selectivity. The nontoxic nature suggests potential for biosensing, such as in vivo imaging and hemoglobin detection.
研究不足
The study is limited to in vitro sensing applications; potential aggregation of C-dots at high pH may affect performance. Optimization of reaction conditions (e.g., temperature, time) is empirical and may not be fully generalized. Toxicity assessments are based on precursor biocompatibility but not extensively tested in biological systems.
1：Experimental Design and Method Selection:
Hydrothermal synthesis was chosen for its ease of operation, low cost, and high efficiency. The method involves heating a mixture of glucose and taurine in water to form C-dots with surface passivation.
2：Sample Selection and Data Sources:
Glucose and taurine were used as precursors. Distilled water was the solvent. Samples were prepared with varying mass ratios (e.g., glucose to taurine ratios from 12:1 to 4:1), reaction temperatures (e.g., 150°C), and reaction times (1-3 hours).
3：List of Experimental Equipment and Materials:
A 50 ml Teflon-lined autoclave for hydrothermal reaction, an oven for heating, an ultrasonic device for mixing, a centrifuge (speed up to 12000 rpm) for separation, UV-Vis absorption spectrometer, photoluminescence (PL) spectrometer, Fourier transform infrared (FTIR) spectrometer, X-ray photoelectron spectroscopy (XPS) instrument, transmission electron microscope (TEM) for characterization, and standard pH buffers and metal ion solutions for sensing experiments.
4：Experimental Procedures and Operational Workflow:
Mix glucose and taurine in distilled water, sonicate, transfer to autoclave, heat at specified temperature and time, cool, centrifuge to remove precipitates, collect supernatant. For sensing: Add Fe3+ ions or pH buffers to C-dots solution, measure fluorescence spectra, analyze intensity changes.
5：Data Analysis Methods:
PL intensity was measured and normalized (F/F0 for quenching studies). Statistical analysis included linear fitting for Fe3+ concentration dependence (correlation coefficient calculated). Instrument software was used for spectral analysis.
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