研究目的
Investigating the effects of low-light fluence rate photodynamic therapy using mTHPC on colon cancer cell lines for potential integration into endoscopic capsules.
研究成果
The research demonstrates that low-light fluence rate PDT with mTHPC can effectively reduce cell viability in colon cancer cells, with optimal conditions at 1–5 μg/mL mTHPC and 2.5 J/cm2 light fluence. This supports the feasibility of integrating PDT into endoscopic capsules for minimally invasive cancer treatment, suggesting future work should focus on in vivo validation and device development.
研究不足
The study is limited to in vitro assays on specific cell lines; in vivo and clinical applications were not tested. The light source and setup may not fully replicate conditions in endoscopic capsules, and further optimization for device integration is needed.
1：Experimental Design and Method Selection:
The study involved in vitro assays on RKO and HCT-15 cell lines using mTHPC as a photosensitizer and a custom LED array for light activation at 653 nm with controlled fluence rates and fluences to assess cell viability.
2：Sample Selection and Data Sources:
Human colon cancer cell lines RKO and HCT-15 were cultured in specific media and seeded in 96-well plates for experiments.
3：List of Experimental Equipment and Materials:
Equipment includes a custom LED array (WP7113SURC/E), monochromator (Oriel Cornerstone 260 ? m), fiber optic (Model: 77533), photodiode detector (Model: 71675), power meter (Model: 1918-R), data acquisition system, aluminum supporting structure, black breadboard, Varioskan Flash Multimode Reader, and materials include mTHPC photosensitizer, methanol, cell culture media, fetal bovine serum, penicillin/streptomycin, phosphate-buffered saline, and MTS assay reagents.
4：Experimental Procedures and Operational Workflow:
Cells were seeded, incubated with mTHPC for 24 hours, washed, irradiated with red light at specified fluences and rates, and cell viability was assessed using MTS assay with absorbance measurement.
5：Data Analysis Methods:
Data were analyzed as average ± standard deviation from three independent assays, normalized to controls, and cell viability was determined based on absorbance at 490 nm.
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