1：Experimental Design and Method Selection:

The study used a custom-built combined laser ray tracing (LRT) and optical coherence tomography (OCT) system to measure lens power in accommodated and unaccommodated states, simulating accommodation with a lens stretcher. The methodology involved automated ray tracing and OCT imaging for precise measurements.

2：Sample Selection and Data Sources:

15 lenses from 11 cynomolgus monkey eyes (Macaca fascicularis, age 3.8–12.4 years, postmortem time 33.5 ± 15.3 hours) were used, obtained from the University of Miami's division of veterinary resources under ethical guidelines.

3：8–4 years, postmortem time 5 ± 3 hours) were used, obtained from the University of Miami's division of veterinary resources under ethical guidelines. List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: Equipment includes a motorized lens stretcher (STR-MLS-B, Bioniko Consulting LLC), LRT-OCT system with components like a CMOS image sensor (DCC1545M-GL, Thorlabs), OCT system (ENVISU R4400, Bioptigen/Leica Microsystems), translation stages (Newport Corp.), rotation stages (Zaber Technologies), and software (LabView, MATLAB). Materials include balanced salt solution (BSS, Alcon Inc.) for hydration.

4：Experimental Procedures and Operational Workflow:

Lenses were mounted in the stretcher, aligned using OCT, and LRT data was acquired at various delivery angles (-20° to +20°) in unstretched and stretched states. The process involved automated ray delivery, spot image acquisition, and data analysis for power calculation.

5：Data Analysis Methods:

Data was analyzed using MATLAB to calculate lens power from ray slopes, with statistical analysis (repeated measures ANOVA) to compare states and assess significance.