1：Experimental Design and Method Selection:

The paper is a review article, so it does not describe a specific experimental design but summarizes existing methods. It discusses PAA templated electrodeposition for fabrication, various stimulation methods for manipulation (e.g., internal gradients, external fields), and imaging techniques like reflectance optical microscopy, Raman intensity mapping, and polarized light microscopy.

2：Sample Selection and Data Sources:

The review cites multiple studies and examples from the literature, such as works by Mirkin et al., Mallouk et al., and others, using fabricated MS-M-NRs with segments like Au, Ni, Pt, etc.

3：List of Experimental Equipment and Materials:

Equipment includes PAA templates, electrochemical setups with electrodes (e.g., platinum wire counter electrode, Ag/AgCl reference electrode), etching processes, optical microscopes, Raman spectrometers, magnetic field generators, and acoustic field devices. Materials involve metallic thin films, sacrificial segments, and various metals for segments.

4：Experimental Procedures and Operational Workflow:

General procedures for fabrication involve electrodeposition into PAA templates, etching to release nanorods. Manipulation involves applying stimuli (e.g., H2O2 for catalysis, acoustic fields, magnetic fields). Imaging involves using specific microscopy techniques with appropriate illumination and polarization.

5：Data Analysis Methods:

Analysis includes observing movements (e.g., speed measurements), imaging identification of segments, and spectroscopic responses, but specific statistical methods are not detailed as it is a review.