研究目的
To develop a novel ZnO/porous carbon matrix (ZnO/MPC)-based electrochemical immunosensor for ultrasensitive detection of C-reactive protein (CRP) using materials obtained via thermolysis of a mixed-ligand MOF.
研究成果
The ZnO/MPC-based immunosensor achieved ultrasensitive detection of CRP with a wide linear range and low detection limit, demonstrating excellent selectivity, reproducibility, and stability. The composite's properties enhance conductivity and biocompatibility, making it promising for future biosensing applications.
研究不足
The semiconducting properties of ZnO may still pose limitations in charge transfer, and the method's applicability to other biomarkers needs further validation. Optimization of parameters such as incubation time and temperature is required for different samples.
1：Experimental Design and Method Selection:
The study involved synthesizing ZnO/MPC composite through thermolysis of a mixed-ligand MOF (Zn-BDC-TED) and constructing an electrochemical immunosensor for CRP detection. Electrochemical techniques such as CV, EIS, and DPV were used to monitor the assembly process and performance.
2：Sample Selection and Data Sources:
Human serum samples were used for practical application analysis, with CRP concentrations spiked for recovery tests.
3：List of Experimental Equipment and Materials:
Chemicals included terephthalic acid, triethylenediamine, Zn(NO3)2·6H2O, DMF, ionic liquid, anti-CRP, BSA, CRP, and electrochemical probes like [Fe(CN)6]3?/4?. Apparatus included a reaction kettle for synthesis, carbon paste electrode (CPE), and electrochemical workstation for measurements.
4：4?. Apparatus included a reaction kettle for synthesis, carbon paste electrode (CPE), and electrochemical workstation for measurements.
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Synthesis of Zn-BDC-TED at 150°C for 6h, thermolysis to ZnO/MPC at 500°C for 2h in N2. Electrode modification involved coating ZnO/MPC/IL on CPE, immobilizing anti-CRP using EDC/NHS, blocking with BSA, and incubating with CRP. Electrochemical measurements were performed in PBS with [Fe(CN)6]3?/4? as the probe.
5：Electrode modification involved coating ZnO/MPC/IL on CPE, immobilizing anti-CRP using EDC/NHS, blocking with BSA, and incubating with CRP. Electrochemical measurements were performed in PBS with [Fe(CN)6]3?/4? as the probe.
Data Analysis Methods:
5. Data Analysis Methods: Linear regression was used for calibration curves (e.g., Ip vs. logc of CRP), and statistical methods like RSD for repeatability and stability assessments.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
