研究目的
This study investigated the effects of a topically applied FO preparation (FOP) on phenol-induced ear edema and evaluated the percutaneous penetration of FOP in ear tissue.
研究成果
The topically applied FOP inhibited leukocyte recruitment in phenol-induced ear edema, as evidenced by reduced MPO activity and PAS band areas. PAS demonstrated percutaneous penetration of FOP and its anti-inflammatory activity, supporting its use as a non-destructive evaluation method for inflammatory responses and compound penetration.
研究不足
The study did not reduce edema with FOP treatment, indicating potential limitations in its efficacy for edema reduction. The use of PAS, while non-destructive, may have sensitivity constraints compared to more advanced imaging techniques. The model is specific to phenol-induced inflammation, which may not generalize to other inflammatory agents.
1：Experimental Design and Method Selection:
The study used a model of phenol-induced ear edema in mice to evaluate the anti-inflammatory effects and percutaneous penetration of a topically applied fish oil preparation (FOP). Photoacoustic spectroscopy (PAS) was employed as a non-destructive method to assess the inflammatory response and penetration.
2：Sample Selection and Data Sources:
Groups of mice were used, with ear tissue collected after 1 hour post-phenol application and FOP treatment. Data included ear weight, myeloperoxidase (MPO) activity, and PAS spectra.
3：List of Experimental Equipment and Materials:
Mice, phenol, fish oil preparation (FOP), olive oil (OO), dexamethasone (Dexa), and photoacoustic spectroscopy equipment.
4：Experimental Procedures and Operational Workflow:
Phenol was applied to induce ear edema, followed by topical application of FOP at various doses. After 1 hour, ear tissue was harvested for edema measurement, MPO activity assay, and PAS analysis. PAS readings were taken from both ventral and dorsal sides of the ear.
5：Data Analysis Methods:
Percent inhibition of edema and MPO activity were calculated. PAS spectra were analyzed using Gaussian deconvolution to identify characteristic bands and assess penetration and anti-inflammatory effects.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
