1：Experimental Design and Method Selection:

A one-pot synthesis method was used to prepare water-soluble and luminescent AgNCs with captopril as the stabilizing agent and NaBH4 as the reductant. The method was optimized for parameters like molar ratio, NaOH concentration, and NaBH4 amount.

2：Sample Selection and Data Sources:

Silver nitrate, captopril, sodium borohydride, and other reagents were used. Solutions were prepared with doubly distilled water. HeLa cells were used for biocompatibility testing.

3：List of Experimental Equipment and Materials:

Equipment included F-4500 fluorescence spectrophotometer, U-2910 spectrophotometer, FLS-920 fluorescence spectrometer, SZCL-3A temperature control stirrer, JEM-2100 TEM, AXIS ULTRA DLD XPS, Bruker IR spectrometer, FE20 pH meter. Materials included AgNO3, NaBH4, captopril, NaOH, BR buffers.

4：Experimental Procedures and Operational Workflow:

Synthesis involved mixing captopril and AgNO3, adjusting pH with NaOH, adding NaBH4, incubating, and dialyzing. Temperature sensing involved mixing AgNCs with water and measuring fluorescence at different temperatures. pH response involved mixing AgNCs with buffers and measuring fluorescence.

5：Data Analysis Methods:

Fluorescence intensity was measured and analyzed for linearity and reversibility. Activation energy was calculated using the Arrhenius equation. Statistical analysis included error bars from three independent measurements.