1：Experimental Design and Method Selection:

A prospective, cross-sectional, noninterventional study design was used. OCT scans were performed sequentially and under standardized conditions using both SD-OCT and SS-OCT devices. Paired t-tests and intraclass correlation coefficients (ICCs) were employed for statistical analysis.

2：Sample Selection and Data Sources:

54 participants were included, comprising 27 normal volunteers and 27 patients with retinal diseases (e.g., diabetic macular edema, ARMD). Inclusion criteria for normal participants: age 20–60 years, normal visual acuity, normal fundus on OCT. Exclusion criteria: history of ocular/systemic diseases, prior intraocular surgery or injections.

3：List of Experimental Equipment and Materials:

SD-OCT machine (Topcon 3D 2000; Topcon Inc., Tokyo, Japan) with wavelength 850 nm and image acquisition rate 50,000 A-scans/sec; SS-OCT machine (Triton; Topcon Inc.) with wavelength 1,050 nm and scanning speed 100,000 A-scans/sec. Built-in caliper tool and software for measurements.

4：Experimental Procedures and Operational Workflow:

Scans performed by a single trained operator under standardized conditions at fixed time (14:00–18:00) to reduce diurnal variation. Sequential scanning by both machines in random order, right eye first. Line scans used: SD-OCT with 6 mm line averaging 50 B-scans, SS-OCT with 12 mm line and 96 B-scans; additional 7x7 scanning protocol on SS-OCT for automatic measurements. Images required minimum score of 45; poor quality images repeated. SFCT measured manually by two graders using caliper tool, and automatically on SS-OCT.

5：Data Analysis Methods:

SPSS 20 used for statistical analysis. Paired t-tests for differences in choroidal thickness (P<0.05 significant). ICCs with 95% CI for intra-reader, inter-reader, and inter-device reliability.