研究目的
Investigating the synthesis and ferric ion modulation of fluorescent nitrogen doped carbon dots for a turn-on assay for ascorbic acid detection.
研究成果
The fluorescence of NCDs is quenched by Fe3+ ions through mechanisms including inner filter effect, aggregation, and ferromagnetism, and this quenching is reversible by AA, enabling a turn-on detection method with good selectivity and sensitivity. The method was successfully applied to spiked human serum and urine samples with good recovery, and test paper strips were developed for visual detection, showing potential for medical diagnostics.
研究不足
The detection limit for AA is 96 μM, which may not be sensitive enough for some applications; interference from certain biomolecules like hemoglobin and some ions could affect selectivity; the method requires specific pH conditions (acidic pH) for optimal performance.
1：Experimental Design and Method Selection:
The study involved synthesizing nitrogen-doped carbon dots (NCDs) from citric acid and urea using a microwave-assisted method, followed by investigating their fluorescence properties, pH dependence, and interaction with metal ions, particularly Fe3+, to develop a fluorescence turn-on sensor for ascorbic acid (AA).
2：Sample Selection and Data Sources:
NCDs were synthesized and characterized; human serum and urine samples were collected from healthy volunteers and spiked with AA for analysis.
3：List of Experimental Equipment and Materials:
Citric acid, urea, FeCl3, ascorbic acid, various biomolecules for interference studies, filter paper for test strips, and instruments such as fluorescence spectrometer, TEM, DLS, FTIR, XPS, and TCSPC.
4：Experimental Procedures and Operational Workflow:
Synthesis of NCDs via microwave heating; fluorescence measurements at different pH and with metal ions; quenching studies with Fe3+; recovery studies with AA; interference tests with other biomolecules; application in spiked biological samples; development of test paper strips.
5：Data Analysis Methods:
Fluorescence intensity measurements, calibration curves, statistical analysis for detection limit and recovery percentages, and spectroscopic data interpretation.
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