研究目的
To examine the test–retest repeatability of microperimetric sensitivity at the border of deep scotomas.
研究成果
Microperimetric test–retest repeatability is worse at the border of deep scotomas compared to normal retinal areas, indicating that a single estimate of repeatability is insufficient for detecting functional decline in these regions. This has implications for clinical monitoring of progressive eye diseases.
研究不足
The study used the ONH in normal participants as a model for deep scotomas, which may not fully replicate pathological conditions. The limited dynamic range of the MP-1 microperimeter introduced ceiling effects, affecting repeatability measurements. Fundus tracking at 25 frames per second may not capture all eye movements, and fixation stability could influence results. Generalization to disease states with different sensitivity gradients is cautioned.
1：Experimental Design and Method Selection:
The study used a test–retest design with two microperimeters (MAIA and MP-1) to assess repeatability at the border of the optic nerve head (ONH) as a model for deep scotomas, along with macular and peripapillary regions. Customized stimulus patterns were designed for practice and formal examinations.
2：Sample Selection and Data Sources:
Thirty normal participants over 18 years old were recruited, with exclusion criteria including ocular pathology, significant cataracts, amblyopia, peripapillary atrophy from disease, diabetes, neurological diseases, or medications affecting vision. The right eye was used for all tests.
3：List of Experimental Equipment and Materials:
MAIA microperimeter (CenterVue), MP-1 microperimeter (Nidek Technologies), Spectralis HRA+OCT unit (Heidelberg Engineering) for SD-OCT scans, customized stimulus patterns, and statistical software (SPSS version 21, IBM).
4：Experimental Procedures and Operational Workflow:
Participants underwent SD-OCT scans to exclude pathology, followed by microperimetry examinations. A practice examination with a movable stimulus pattern was performed first, then two formal examinations per microperimeter. Stimulus patterns were placed to sample the ONH border, macular, and peripapillary regions. False-positive responses were monitored for reliability.
5：Data Analysis Methods:
Linear mixed effects models were used to assess changes in average point-wise sensitivity (PWS). Bland-Altman plots and coefficients of repeatability (CoR) were calculated. Statistical comparisons used Kruskal-Wallis tests with Bonferroni corrections, and normality was checked with Shapiro-Wilk tests.
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