1：Experimental Design and Method Selection:

The study involved growing B2ABP single crystals using solvent evaporation method, followed by characterization using various techniques including X-ray diffraction, FT-IR, Raman, NMR, UV-Vis-NIR, photoluminescence, Vickers microhardness, etching, dielectric measurements, SHG efficiency, laser damage threshold, TG-DTA, and DFT calculations for HOMO-LUMO and hyperpolarizability. Anti-microbial activity was assessed using broth dilution, antibiotic sensitivity assay, and motility test.

2：Sample Selection and Data Sources:

Commercially available 2-aminobenzimidazole (Sigma Aldrich) and phthalic acid (Alfa-Aesar) were used in a 2:1 ratio dissolved in methanol. Crystals were grown and purified by recrystallization.

3：List of Experimental Equipment and Materials:

Instruments included Enraf-Nonius CAD4 diffractometer, PAN analytical X-pert pro diffractometer, Shimadzu spectrometer, Lab RAM HR 800 Raman spectrometer, Bruker 300 MHz NMR spectrometer, Shimadzu UV-Vis-NIR spectrophotometer, Perkin Elmer spectrofluorometer (Model: LS 45), MATSUZAWA MMTX-7 series hardness tester, Carl Zeiss optical microscope, HIOKI 3532-50 LCR meter, Q-switched Nd:YAG laser, Exstar 6000 TG-DTA instrument, and Gaussian 03 software. Materials included methanol, KBr pellets, Deuterated DMSO, Mueller Hinton Broth, and silver paste.

4：Experimental Procedures and Operational Workflow:

Synthesis involved mixing solutions, stirring, evaporation, and crystal growth over 25 days. Characterization steps included XRD for structure confirmation, spectroscopic analyses for functional groups and structure, optical measurements for transmittance and emission, mechanical testing for hardness, etching for defect analysis, dielectric studies at various frequencies and temperatures, SHG and laser damage measurements, thermal analysis, DFT calculations, and biological assays for anti-microbial activity.

5：Data Analysis Methods:

Data were analyzed using Mercury software for XRD indexing, Gaussian 03 for DFT calculations, and standard formulas for optical band gap, hardness, SHG efficiency, laser damage threshold, and other parameters. Statistical analysis was performed in triplicate for biological assays.