研究目的
To develop ginsenoside compound K-loaded gold nanoparticles using Lactobacillus kimchicus DCY51T for enhanced drug delivery and photothermal therapy in cancer treatment.
研究成果
DCY51T-AuCKNps are promising drug delivery platforms for cancer therapy, exhibiting enhanced cytotoxicity and apoptosis in cancer cells under laser irradiation, with potential for photothermal and chemotherapeutic synergy.
研究不足
Preliminary in vitro study; lack of in vivo experiments; potential issues with nanoparticle clearance from the body; need for specific targeting to minimize toxicity.
1：Experimental Design and Method Selection:
One-pot biosynthesis using Lactobacillus kimchicus DCY51T to synthesize gold nanoparticles loaded with ginsenoside CK, characterized by various techniques including FE-TEM, EDX, XRD, SAED, FTIR, DLS, and LC-MS. In vitro studies on cytotoxicity and photothermal effects in cancer cell lines.
2：Sample Selection and Data Sources:
Bacterial strain isolated from Korean kimchi, cancer cell lines (A549, HT29, AGS, RAW264.7, HaCaT) obtained from KCLB and ATCC.
3：7, HaCaT) obtained from KCLB and ATCC. List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: MRS broth and agar, hydrogen tetrachloroaurate (III) hydrate, ginsenoside CK, doxorubicin, rifaximin, PBS, MeOH, DMEM, FBS, RPMI 1640, MTT, lasers (635 nm and 800 nm), FE-TEM (JEM-2100F), UV-vis spectrophotometer (UltrospecTM 2100 pro), XRD (D8 Discover), DLS (ELS-Z2), FTIR (PerkinElmer Spectrum 100), LC-MS (Agilent Technologies 6410 Triple Quad), ICP/QMS (NexION 300D), fluorescence microscope (Optinity), TIRS/DIC system (Olympus BX51).
4：1). Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Bacterial culture, synthesis of nanoparticles, characterization, stability tests, cytotoxicity assays (MTT), photothermal therapy with laser irradiation, apoptosis detection (Hoechst staining), data analysis.
5：Data Analysis Methods:
Statistical analysis using Student's t-test, data presented as means ± SDs, drug loading efficiency calculated from LC-MS results.
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Field Emission Transmission Electron Microscope
JEM-2100F
JEOL
Used for high-resolution imaging and analysis of nanoparticles.
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X-ray Diffractometer
D8 Discover
Bruker
Used for XRD analysis to determine crystalline nature of nanoparticles.
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Fourier-Transform Infrared Spectrometer
Spectrum 100
PerkinElmer
Used for FTIR analysis to identify functional groups.
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Liquid Chromatography-Mass Spectrometry
6410 Triple Quad
Agilent Technologies
Used for drug loading efficiency determination.
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Inductively Coupled Plasma/Quadrupole-Mass Spectrometer
NexION 300D
PerkinElmer
Used to measure gold content in nanoparticles.
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Total Internal Reflection Scattering Microscope
BX51
Olympus
Used for TIRS microscopy to detect nanoparticles.
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Ultraviolet-Visible Spectrophotometer
UltrospecTM 2100 pro
Amersham Biosciences
Used to measure absorbance spectra of nanoparticles.
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Zeta Potential and Particle Size Analyzer
ELS-Z2 series
Otsuka Electronics
Used to measure zeta potential and hydrodynamic size of nanoparticles.
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Enzyme-Linked Immunosorbent Assay Reader
Bio-Tek Instruments
Used to quantify absorbance in MTT assays.
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Fluorescence Microscope
Optinity
Korean Labtech
Used for imaging Hoechst-stained cells.
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Laser
635 nm
Shanghai Dream Laser Technology
Used for photothermal therapy irradiation.
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Laser
800 nm
Used for photothermal therapy irradiation in AGS cells.
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Mechanical Shutter
LS3S2ZO-R3
Vincent Associates
Synchronized with camera for TIRS microscopy.
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Camera
QuantEM 512SC
Electron-multiplying cooled charge-coupled device camera for imaging.
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Software
MetaMorph 7.5
Universal Imaging
Used for image acquisition and analysis.
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