1：Experimental Design and Method Selection:

The study involved synthesizing MnPc nanosheets via solvothermal reaction, modifying with HA for targeting, loading curcumin as a chemotherapy drug, and evaluating photothermal effects, drug release, tumor targeting, biocompatibility, in vitro and in vivo antitumor activity, and MR imaging capabilities. Theoretical models include coordination chemistry for nanosheet formation and photothermal conversion efficiency calculations.

2：Sample Selection and Data Sources:

Samples included MnPc tetracarboxylic acid, zirconyl chloride, hyaluronic acid, curcumin, and cell lines (4T1, NIH/3T3, HeLa, L929) and BALB/c mice for in vivo studies. Data were acquired through various spectroscopic, microscopic, and imaging techniques.

3：List of Experimental Equipment and Materials:

Equipment included TEM, AFM, DLS, XPS, UV-vis spectrometer, FTIR, thermal imaging camera, NMR analyzer, MR scanner, confocal microscope, fluorescence microscope, flow cytometer, atomic absorption spectrometer, and ICP-MS. Materials included DMSO, DMF, ethanol, PBS, FBS, HAase, and various chemicals for synthesis and assays.

4：Experimental Procedures and Operational Workflow:

Synthesis of MnPc nanosheets at 90°C for 12 h, drug loading by sonication and stirring, photothermal experiments with NIR light irradiation, drug release studies under different conditions, cellular uptake and intracellular release assays, in vitro cytotoxicity and live/dead staining, in vivo MR imaging and antitumor therapy in mice with monitoring of tumor size and body weight.

5：Data Analysis Methods:

Data were analyzed using UV-vis and fluorescence spectrometry for quantification, statistical methods for cell viability, and software for imaging analysis (e.g., flow cytometry for fluorescence intensity, MR imaging software for signal quantification).