1：Experimental Design and Method Selection:

The study involved synthesizing carboranyl carbazoles via Suzuki coupling reactions, characterizing them using NMR, IR, and HRMS, and investigating their photophysical and ECL properties. ECL experiments were conducted using a three-electrode system with cyclic voltammetry to study redox behavior.

2：Sample Selection and Data Sources:

Compounds T-1 to T-6 were synthesized and tested. Samples were dissolved in THF or THF/H2O mixtures with varying water fractions to induce aggregation.

3：List of Experimental Equipment and Materials:

Equipment includes transmission electron microscope (TEM) for morphology, dynamic light scattering (DLS) for particle size, cyclic voltammetry setup with Pt counter electrode, Ag/AgCl reference electrode, glassy carbon electrode (GCE) as working electrode, and photomultiplier tube (PMT) for ECL detection. Materials include carboranyl carbazoles, tetraoctylammonium bromide (TOAB) as conductive reagent, potassium persulfate (K2S2O8) as co-reactant, and phosphate-buffered saline (PBS).

4：Experimental Procedures and Operational Workflow:

Compounds were dissolved, sonicated, pipetted onto GCE, dried, and ECL intensity measured in PBS with K2S2O8. Water fractions were varied to study aggregation effects. CV was performed in DMF with TBAP.

5：Water fractions were varied to study aggregation effects. CV was performed in DMF with TBAP. Data Analysis Methods:

5. Data Analysis Methods: Data analysis involved comparing ECL intensities, quantum yields, particle sizes from TEM and DLS, and CV peaks to propose mechanisms.