1：Experimental Design and Method Selection:

The study used a case-control design to compare mfERG responses between regular cannabis users and healthy controls. mfERG was performed in accordance with International Society for Clinical Electrophysiology of Vision (ISCEV) standards to assess spatial localization of retinal anomalies.

2：Sample Selection and Data Sources:

49 regular cannabis users and 21 matched healthy drug-naive controls were recruited from a previous study. Inclusion criteria included no DSM-IV Axis I disorders, assessed using the Mini-International Neuropsychiatric Interview (M.I.N.I.).

3：List of Experimental Equipment and Materials:

MonPackONE system (Metrovision, France) for stimulation, recording, and analysis; Dawson-Trick-Litzkow (DTL) electrodes; Tropicamide 0.5% for pupil dilation; ground and reference electrodes; stimulus matrix with 61 scaled hexagonal elements; Monitor Ophthalmic software (Metrovision, France) for data analysis; IBM-SPSS Statistics

4：5% for pupil dilation; ground and reference electrodes; stimulus matrix with 61 scaled hexagonal elements; Monitor Ophthalmic software (Metrovision, France) for data analysis; IBM-SPSS Statistics 0 for statistical analysis. Experimental Procedures and Operational Workflow:

22.0 for statistical analysis. 4. Experimental Procedures and Operational Workflow: Pupils were dilated with Tropicamide 0.5%; DTL electrodes placed at the bottom of the conjunctival sac; ground and reference electrodes fixed to forehead and external canthi; stimulus matrix modulated between white and black with luminance of 100 cd/m2 and background at 30 cd/m2; stimuli frequency at 75 Hz; participants positioned 30 cm from screen with optical correction; fixation on central red target; segments with blinks or eye movements rejected; at least 5000 responses recorded per eye with noise under 5 kΩ; mfERG responses averaged over five retinal regions (b2°, 2–5°, 5–10°, 10–15°, N15°); analysis of N1, P1, N2 amplitudes and implicit times; experimenter blinded to subject status.

5：5%; DTL electrodes placed at the bottom of the conjunctival sac; ground and reference electrodes fixed to forehead and external canthi; stimulus matrix modulated between white and black with luminance of 100 cd/m2 and background at 30 cd/m2; stimuli frequency at 75 Hz; participants positioned 30 cm from screen with optical correction; fixation on central red target; segments with blinks or eye movements rejected; at least 5000 responses recorded per eye with noise under 5 kΩ; mfERG responses averaged over five retinal regions (b2°, 2–5°, 5–10°, 10–15°, N15°); analysis of N1, P1, N2 amplitudes and implicit times; experimenter blinded to subject status. Data Analysis Methods:

5. Data Analysis Methods: Statistical analysis using Mann-Whitney U test, Chi-square test, and Spearman rank correlation coefficient as appropriate; categorical variables as numbers, quantitative variables as median and interquartile range; significance level alpha < 0.05.