研究目的
To present a protocol for conducting fNIRS hyperscanning experiments with parent-child dyads and for analyzing brain-to-brain synchrony, aiming to understand the neurobiological underpinnings of social interactions.
研究成果
fNIRS hyperscanning is a promising technique for studying brain-to-brain synchrony during social interactions, offering insights beyond individual brain activities. Future directions include using portable devices, combining with other neuroimaging techniques, and improving analysis methods.
研究不足
fNIRS is limited to cortical surface measurements, prone to motion artifacts especially with children, sensitive to hair type affecting optode contact, lacks standardized data analysis guidelines, and requires careful control for physiological confounders like heart rate synchronization.
1:Experimental Design and Method Selection:
The protocol involves using fNIRS for hyperscanning to measure hemodynamic responses in prefrontal brain regions during cooperative and competitive tasks. It includes steps for data collection, preprocessing, bad channel detection, and wavelet coherence analysis.
2:Sample Selection and Data Sources:
Parent-child dyads, with children aged five to nine years, are used. Data is acquired from fNIRS signals.
3:List of Experimental Equipment and Materials:
Includes fNIRS measurement system (e.g., ETG-4000), EEG caps modified for NIRS, probe holder grids, chin rests, computer with Psychophysics Toolbox, and software for data analysis (e.g., SPM for fNIRS, ASToolbox).
4:Experimental Procedures and Operational Workflow:
Steps include preparing NIRS caps, setting up the fNIRS system, placing caps on participants, running cooperative and competitive tasks, and saving raw data.
5:Data Analysis Methods:
Preprocessing using modified Beer-Lambert law, motion artifact reduction, bad channel detection based on coefficient of variation and visual inspection, and wavelet coherence analysis to measure brain-to-brain synchrony.
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NIRS measurement system
ETG-4000
Hitachi
Measures hemodynamic responses using near-infrared spectroscopy for brain activity recording.
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EEG cap
Modified to hold NIRS probes for hyperscanning experiments.
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Probe holder grid
3x5 grid
Holds emitter and detector probes in place on the cap.
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Chin rest
Prevents head movements during the experiment.
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Psychophysics Toolbox
Version 3.0.11
Software for programming and running the experimental paradigm.
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Technical computing software
MATLAB
Base software for running Psychophysics Toolbox and data analysis scripts.
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SPM for fNIRS
Software toolbox for fNIRS data preprocessing and analysis.
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ASToolbox
Software for computing wavelet transform coherence in data analysis.
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