研究目的
To develop a simple and efficient fluorescent sensor for the sensitive and selective detection of environmental toxicant Hg2+ in real-time, with applications in environmental monitoring and biological imaging.
研究成果
The PST sensor demonstrates high selectivity and sensitivity for Hg2+ detection with a low LOD and reversible binding. It is effective for environmental monitoring via test strips and biological imaging in live cells, offering a practical 'two in one' kit for real-time applications.
研究不足
The sensor operates in a specific solvent mixture (MeCN-H2O 8:2 v/v), which may limit its applicability in purely aqueous environments. The LOD of 0.63 μM might not be sufficient for ultra-trace detection, and the study primarily focuses on Hg2+, with potential interference from other ions not fully explored in complex matrices.
1：Experimental Design and Method Selection:
The study involved synthesizing a rhodamine-based sensor (PST) and evaluating its response to Hg2+ using fluorescence and UV-Vis spectroscopy. Theoretical models like density functional theory (DFT) were used to support binding mechanisms.
2：Sample Selection and Data Sources:
Solutions of PST and various metal ions were prepared in MeCN-H2O (8:2 v/v). HeLa cells were used for biological studies.
3：List of Experimental Equipment and Materials:
Instruments included PerkinElmer LS 55 fluorescence spectrophotometer, Schimadzu UV-2600 spectrophotometer, Varian mercury plus 400 NMR spectrometer, Agilent 1100 MS series, Leica DMI-6000 confocal microscope. Chemicals included rhodamine B, 2-amino-5-bromopyridine, metal salts, EDTA, etc.
4：Experimental Procedures and Operational Workflow:
Synthesis of PST via multi-step reactions, spectral titrations with Hg2+, selectivity tests with other metals, reversibility tests with EDTA, DFT calculations, cell imaging with HeLa cells, and test strip experiments.
5：Data Analysis Methods:
Binding constants and LOD were calculated using nonlinear least square analysis and standard deviation methods. Spectroscopic data were analyzed for intensity changes.
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