研究目的
To describe the methodology for preclinical FDG PET/CT imaging in cancer metabolism studies, including considerations for alternative metabolic PET tracers.
研究成果
The chapter provides a comprehensive protocol for conducting preclinical FDG PET/CT imaging to study cancer metabolism, emphasizing the importance of proper scanner calibration, animal preparation, and data analysis. It highlights the utility of PET for noninvasive metabolic assessment and discusses considerations for using alternative tracers. Future studies should focus on optimizing tracer development, improving model relevance, and advancing quantitative imaging techniques to better understand cancer metabolism in vivo.
研究不足
Technical constraints include the short half-lives of radiotracers (e.g., F18: 109.7 min, C11: 20.4 min) requiring rapid work, need for specialized radiochemistry facilities for non-FDG tracers, and potential metabolic perturbations from anesthesia and handling. Application constraints involve reliance on mouse models, which may not fully replicate human cancer, and challenges in image reconstruction and analysis. Areas for optimization include improving tracer availability, refining anesthesia protocols, and enhancing image quality and quantification methods.
