1：Experimental Design and Method Selection:

The study involved designing and synthesizing the fluorescent probe DPD based on perylenequinone. The detection capabilities were evaluated using UV-vis and fluorescence spectroscopy, with mechanisms confirmed by 1H NMR. Cell imaging experiments were conducted to assess applicability in living cells.

2：Sample Selection and Data Sources:

HeLa cells were used for confocal imaging. Analytes included various anions, cations, amines, and amino acids to test selectivity. Stock solutions were prepared in distilled water or DMSO.

3：List of Experimental Equipment and Materials:

Equipment included a Bruker (500 MHz) NMR instrument, PerkinElmer LS-55 spectrometer, PerkinElmer Lambda 950 spectrophotometer, Delta 320 pH-meter, Leica TCS SPE confocal scanning microscope, and ZEISS LSM 710 Confocal Microscope. Materials included DPD, NaHS, and other analytes from commercial suppliers.

4：Experimental Procedures and Operational Workflow:

DPD stock solution was prepared in DMSO. Analytes were added to DPD solutions, and spectra were recorded after 2 min. Cell experiments involved incubating HeLa cells with DPD and NaHS, followed by confocal imaging.

5：Data Analysis Methods:

Fluorescence and absorption spectra were analyzed to determine selectivity, sensitivity, response time, and pH dependence. Detection limit was calculated using LOD=3×δblank/k.