1：Experimental Design and Method Selection:

The study employed an in-situ growth method to integrate MOF nanoparticles into inorganic nanomaterials (MnO2). This involved synthesizing ZIF-8 and ZrMOF nanoparticles, then growing MnO2 nanodots or nanosheets on their surfaces to form core-satellites or core-shell structures. The fluorescence and photodynamic activity were controlled by MnO2 quenching and GSH reduction. In vitro studies included fluorescence spectroscopy, singlet oxygen generation assays, cytotoxicity tests, and MRI relaxivity measurements. In vivo studies used U87MG tumor-bearing mice to evaluate photodynamic therapy efficacy and MRI-guided therapy.

2：2). This involved synthesizing ZIF-8 and ZrMOF nanoparticles, then growing MnO2 nanodots or nanosheets on their surfaces to form core-satellites or core-shell structures. The fluorescence and photodynamic activity were controlled by MnO2 quenching and GSH reduction. In vitro studies included fluorescence spectroscopy, singlet oxygen generation assays, cytotoxicity tests, and MRI relaxivity measurements. In vivo studies used U87MG tumor-bearing mice to evaluate photodynamic therapy efficacy and MRI-guided therapy. Sample Selection and Data Sources:

2. Sample Selection and Data Sources: Samples included synthesized ZIF-8, ZrMOF, ZIF-FITC@MnO2, and ZrMOF@MnO2 nanoparticles. Data were obtained from laboratory syntheses and characterizations (e.g., TEM, HAADF-STEM, XPS, DLS, UV-vis, fluorescence spectroscopy, LC-MS, ICP). In vivo data came from mice models with U87MG tumors.

3：List of Experimental Equipment and Materials:

Equipment: Transmission electron microscopy (TEM), high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM), X-ray photoelectron spectroscopy (XPS), dynamic light scattering (DLS), UV-vis spectrophotometer, fluorescence spectrometer, LC-MS system, ICP instrument, MRI system, confocal microscope (Zeiss LSM 780), plate reader. Materials: Zn(NO3)2, 2-methylimidazole, ZrOCl2, meso-tetra(4-carboxyphenyl)porphyrin (TCPP), benzoic acid, KMnO4, GSH, SOSG, MTT, DAPI, Calcium AM/PI, PEG, DMF, methanol, water, sodium acetate, PBS.

4：Experimental Procedures and Operational Workflow:

Synthesis of ZIF-8 and ZrMOF nanoparticles was performed in methanol or DMF with specific reactants and conditions. In-situ growth of MnO2 involved adding aqueous KMnO4 to nanoparticle solutions with stirring. Fluorescence and singlet oxygen generation were measured with and without GSH treatment. Cytotoxicity was assessed via MTT assay and live/dead staining after laser irradiation. In vivo studies involved intravenous injection of PEGylated nanoparticles into mice, followed by laser irradiation and MRI imaging.

5：Data Analysis Methods:

Data analysis included fluorescence intensity measurements, singlet oxygen quantification using SOSG, cell viability calculation from MTT absorbance, flow cytometry for apoptosis, ICP for Mn release, MRI relaxivity (r1) calculation, and statistical analysis of tumor growth and survival rates.