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Observation of Acetylcholinesterase in Stress-induced Depression Phenotypes by Two-photon Fluorescence Imaging in the Mouse Brain
摘要： Oxidative stress in depression is a prime cause of neurotransmitter metabolism dysfunction in the brain. Acetylcholinesterase (AChE), a key hydrolase in the cholinergic system, directly determines the degradation of neurotransmitters. However, due to the complexity of the brain and lack of appropriate in situ imaging tools, the mechanism underlying the changes in AChE activity in depression remains unclear. Hence, we generated a two-photon fluorescence probe (MCYN) for real-time visualization of AChE with excellent sensitivity and selectivity. AChE can specifically recognize and cleave the carbamic acid ester bond in MCYN, and MCYN emits bright fluorescence at 560 nm by two-photon excitation at 800 nm. By utilizing MCYN to monitor AChE, we discovered a significant increase in AChE activity in the brains of mice with depression phenotypes. Notably, with the assistance of a two-photon fluorescence imaging probe of the superoxide anion radical (O2??), in vivo visualization for the first time revealed the positive correlation between AChE and O2?? levels associated with depressive behaviors. This finding suggests that oxidative stress may induce AChE overactivation, leading to depression-related behaviors. This work provides a new and rewarding perspective to elucidate the role of oxidative stress regulating AChE in the pathology of depression.
关键词： acetylcholinesterase,depression,Two-photon fluorescence imaging,brain,oxidative stress
更新于2025-09-23 15:23:52
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Protective role of melatonin on retinal ganglionar cell: In vitro an in vivo evidences
摘要： Oxidative stress triggers ocular neurodegenerative diseases, such as glaucoma or macular degeneration. The increase of reactive oxygen and nitrogen species in retinal ganglion cells (RGCs) causes damage to the structure and function of the axons that make up the optic nerve, leading to cell death arising from apoptosis, necrosis or autophagy in the RCGs. The use of antioxidants to prevent visual neurodegenerative pathologies is a novel and possibly valuable therapeutic strategy. To investigate in vitro and in vivo neuroprotective efficacy of melatonin (MEL) in RGCs, we used a model of oxidative glutamate (GLUT) toxicity in combination with L-butionin-S, R-sulfoximine (BSO), which induces cell death by apoptosis through cytotoxicity and oxidative stress mechanisms. Histological sectioning and immunohistochemical assays using the TUNEL technique were performed to determine the damage generated in affected cells and to observe the death process of RGCs. Whit BSO-GLUT the results revealed a progressive RGCs death without any significant evidence of a decreased retinal function after 9 days of treatment. In this way, we were able to develop a retinal degeneration model in vivo to carry out treatment with MEL and observed an increase in the survival percentage of RGCs, showing that BSO-GLUT could not exert an oxidant effect on cells to counteract the effect of MEL. These findings reveal that MEL has a neuroprotective and antiapoptotic effect as evidenced by the reduction of oxidative stress damage. MEL demonstrated in this model makes it a promising neuroprotective agent for the treatment of ocular neurodegenerative diseases when administered locally.
关键词： Ganglion retinal cells (RGCs),Oxidative stress,Neuroprotection,Melatonin
更新于2025-09-23 15:23:52
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[IEEE 2018 Seventh Balkan Conference on Lighting (BalkanLight) - Varna (2018.9.20-2018.9.22)] 2018 Seventh Balkan Conference on Lighting (BalkanLight) - Investigation of Reliability of Diversity Systems Through Stress-Strength Model Analysis
摘要： Paper concerns a problem of reliability indexes calculation of two channel homogeneous and diversity system. Using SSM it is easy to determine the probability of Common Cause Failures (CCF) and other parameters of investigated redundant systems. Here is used a complex approach with Multi-Factor Stress-Strength Model (MFSSM). The obtained analytical formulas are verificated via simulation.
关键词： diversity,common cause failures,stress-strength model,dependability
更新于2025-09-23 15:23:52
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[IEEE 2018 International Multi-Conference on Industrial Engineering and Modern Technologies (FarEastCon) - Vladivostok, Russia (2018.10.3-2018.10.4)] 2018 International Multi-Conference on Industrial Engineering and Modern Technologies (FarEastCon) - Experimental Study of the Stress-Strain State Features of Outlet Guide Vane Made From Polymer Composite Material Using Fiber Optic Sensors
摘要： An estimation technique for the stress-strain state of the outlet guide vane design of an aircraft propulsion system made of polymer composite materials (PCM) using fiber-optic sensors is proposed. Fiber-optic sensors allow creating details with the possibility of self-diagnosis and forecasting the resource of work. This issue is particularly relevant when using PCM in highly loaded aircraft engine elements. The object of the study is the outlet guide vane (OGV) made of an equally strong carbon cloth on an epoxy binder. The tests were carried out on a universal servo-hydraulic machine. The data from fiber-optic sensors was carried out using an interrogator ASTRO X327. The comparison of the experiment and the numerical calculation were performed using the software package ANSYS.
关键词： polymer composite material,fiber optic sensors,numerical calculations,stress-strain state
更新于2025-09-23 15:23:52
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Characterization of the Diamond Wire Sawing Process for Monocrystalline Silicon by Raman Spectroscopy and SIREX Polarimetry
摘要： A detailed approach to evaluate the sub-surface damage of diamond wire-sawn monocrystalline silicon wafers relating to the sawing process is presented. Residual stresses, the presence of amorphous silicon and microcracks are considered and related to diamond wire velocity and cutting ability. In particular, the degree of amorphization of the wafer surface is analyzed, as it may affect the etching performance (texturing) during solar cell manufacture. Raman spectroscopy and Scanning Infrared Stress Explorer (SIREX) measurements are used independently as non-destructive, contactless optical characterization methods to provide stress imaging with high spatial resolution. Raman mappings show that amorphous silicon layers can occur inhomogeneously across the surface of diamond wire-sawn wafers. The Raman and SIREX results reveal a connection between a higher fraction of the amorphous phase, a more inhomogeneous stress distribution and a lower peak maximum of the stress difference on wafers, depending on both the wire wear and the wire velocity. SIREX line scans of the in-plane difference of the principal stress components ?σ taken across the sawing grooves show significant differences in magnitude and periodicity. Furthermore, the results are compared with the microcrack depth from the same investigation areas. The possibility to optimize the diamond wire sawing processes by analyzing the sub-surface stress of the wafers is offered by complementary use of both Raman and SIREX measurements.
关键词： SIREX,wire cutting ability,silicon,microcrack depth,diamond wire,amorphous phase,wire velocity,Raman,stress imaging,stress-induced birefringence
更新于2025-09-23 15:22:29
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Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response
摘要： Biological phase transitions form membrane-less organelles that generate distinct cellular environments. How molecules are partitioned between these compartments and the surrounding cellular space and the functional consequence of this localization is not well understood. Here, we report the localization of mRNA to stress granules (SGs) and processing bodies (PBs) and its effect on translation and degradation during the integrated stress response. Using single mRNA imaging in living human cells, we find that the interactions of mRNAs with SGs and PBs have different dynamics, very few mRNAs directly move between SGs and PBs, and that specific RNA-binding proteins can anchor mRNAs within these compartments. During recovery from stress, we show that mRNAs that were within SGs and PBs are translated and degraded at similar rates as their cytosolic counterparts. Our work provides a framework for using single-molecule measurements to directly investigate the molecular mechanisms of phase-separated compartments within their cellular environment.
关键词： P-bodies,integrated stress response,degradation,stress granules,LARP1,mRNA localization,single-molecule imaging,translation
更新于2025-09-23 15:22:29
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Numerical and experimental analysis of the effect of metal tie layers on improving electrical properties of gold coated poly (dimethylsiloxane) flexible multi-layered films during folding
摘要： To improve folding electrical properties of flexible electronics, this study presents a new adhesive stress analysis of depositing an adhesion layer between the conductive film electrode and the flexible substrate during folding test by a folding test platform. The folding test platform controls folding curvature of the flexible conductive substrates, folding times, and velocities in this study. Moreover, the electrical properties of flexible conductive substrates are measured during folding testing by the folding test platform. Eventually, this study successfully analyzes residual strain, adhesive stress, and electrical properties of flexible gold coated on polydimethylsiloxane substrates with chromium, nickel, and titanium adhesion layers up to 0.5/cm folding curvature during folding testing. The chromium adhesion layer had the best performance based on the largest adhesive stress, only 3.74 X resistance increase and 4.53 X maximum resistance up to 0.5/cm folding curvature during folding test by a folding test platform. The experimental result with chromium adhesion layer is consistent with adhesive stress analysis and provides a better adhesive strength between gold and polydimethylsiloxane than nickel and titanium materials for folding test. Therefore, adhesive stress analysis is adapted to evaluate the foldable electronics performance for improving folding characteristics easier.
关键词： flexible electronics,Folding electrical property,folding test platform,adhesive stress analysis,adhesion layer
更新于2025-09-23 15:22:29
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Size-Dependent Buckling and Vibrations of Piezoelectric Nanobeam with Finite Element Method
摘要： In the present paper, a finite element method is used to study the vibrations and buckling of a piezoelectric nanobeam. The beam theory used here is Bernoulli–Euler model. In order to achieve the goal, first, the governing equations of the piezoelectric nanobeam were obtained via the Hamilton principle, based on the higher-order theory such as modified couple stress. The shape functions were embedded in these equations, and the matrix form of the equations was obtained. In other words, the governing equations were discretized by the finite element method. By calculating the matrix form of equations, mass matrices, mechanical stiffness and electrical stiffness of the beam were obtained. It should be noted that when calculating stiffness matrices, first, the definition of a new element must be added to the effects of the electric field in the elements of the beam, and the effects of the gradient of strain should be added in the form of non-classical matrix to the stiffness matrix. With the aid of these critical load matrices, the buckling force and voltage and the natural frequency of the piezoelectric nanobeam have been calculated. Numerical results show that this method can involve the size effects.
关键词： Size effect,Piezoelectric,Buckling voltage,Couple stress theory,Free vibration,Buckling load
更新于2025-09-23 15:22:29
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Modes of vibration, stability and internal resonances on non-linear piezoelectric small-scale beams
摘要： This paper presents a non-linear piezoelectric size-dependent Rayleigh’s beam model, based on modified couple stress theory, a non-classical continuum theory that is sensitive to the size effect. Hamilton principle is employed to determine the partial differential governing equations and the boundary conditions. Free periodic vibrations are studied, with the goal of investigating the modes of vibration of piezoelectric small-scale beams in the non-linear regime, a study that appears to be lacking in the literature. The method of multiple scales and a shooting method are applied to solve the equations of motion; the stability of the solutions computed by the shooting method is investigated. A hinged-hinged beam is chosen as an example to delineate the non-linear size dependent free vibration behaviour. For the first time in this specific problem, secondary branches due to bifurcations are found. An important novelty of the work is that these secondary branches are determined using the shooting method, which does not suffer from the drawbacks of popular alternatives, as perturbation methods and the method of harmonic balance.
关键词： Piezoelectric,Modified couple stress,Non-linear modes of vibration,Small scale beams
更新于2025-09-23 15:22:29
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[IEEE 2018 IEEE International Ultrasonics Symposium (IUS) - Kobe, Japan (2018.10.22-2018.10.25)] 2018 IEEE International Ultrasonics Symposium (IUS) - Accelerated Aging Procedures of Bending Piezoelectric Structures Using Electrical Stress Induced Approaches
摘要： Efficient AgInGaS quantum dot-based energy harvesting devices using electrical and mechanical approaches
关键词： energy harvesting,quantum dots,optoelectronics,AgInGaS,mechanical stress
更新于2025-09-23 15:22:29