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Laser Patterned ZNO Substituted Calcium Phosphate Scaffolds via Viscous Polymer Processing for Bone Graft
摘要： Polymer dough processing is a method which can easily overcome this problem. In current study ZnO doped calcium phosphate (Zn-CaP) micropatterned scaffold was explored through viscous polymer processing route. Zn-CaP nanopowder was synthesized using the wet chemical route. It was characterized using SEM, TEM, XRD and EDX. Highly ductile micro-patterned bone grafts were constructed using ZnO doped calcium phosphate scaffolds using roll milling followed by Laser machining. It was kept for cell culture and cytocompatibility using MTT, DNA quantification, and ALP assay. Hemolysis and blood cell aggregation assay was conducted for in vivo applicability. The cellular growth was also observed using fluorescence microscopy, live-dead assay, and SEM. Further gene expression was analyzed using PCR studies which showed osteogenic differentiation of bone marrow-derived stem cells without any supplemented media. The material showed a significant zone of inhibition against both gram positive and negative bacteria. Selective cellular growth was observed at micropatterned zone.
关键词： Doping,Antimicrobial activity,Bioactivity,Micropatterning,Viscous polymer processing
更新于2025-09-16 10:30:52
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Investigating the efficiency of α-Bismuth zinc oxide heterostructure composite/UV-LED in methylene blue dye removal and evaluation of its antimicrobial activity.
摘要： Heterostructured α-Bismuth zinc oxide (α-Bi2O3-ZnO) photocatalyst was fabricated by a facile and cost-effective, ultrasound assisted chemical precipitation method followed by hydrothermal growth technique. As synthesized α-Bi2O3-ZnO photocatalyst showed enhanced photocatalytic performance for the MB dye degradation in contrast to pure ZnO and α-Bi2O3. Light emitting diodes (UV-LED) were used in the experimental setup, which has several advantages over conventional lamps like wavelength selectivity, high efficacy, less power consumption, long lifespan, no disposal problem, no warming-up time, compactness, easy and economic installation. XRD study confirmed the presence of both the lattice phases i.e. monoclinic and hexagonal wurtzite phase corresponding to α-Bi2O3 and ZnO in the α-Bi2O3-ZnO composite photocatalyst. FESEM images showed that α-Bi2O3-ZnO photocatalyst is composed of dumbbell like structures of ZnO with breadth ranging 4-5 μm and length ranging from 10 to 11 μm respectively. It was observed that α-Bi2O3 nanoparticles were attached on the ZnO surface and were in contact with each other. Low recombination rate of photo-induced electron-hole pairs, due to the migration of electrons and holes between the photocatalyst could be responsible for the 100 % photocatalytic efficiency of α-Bi2O3-ZnO composite. In addition, photocatalyst was also observed to show the excellent antimicrobial activity with 1.5 cm zone of inhibition for 1 mg/L dose, against the human pathogenic bacteria (S. aureus).
关键词： Heterostructure,antimicrobial activity,Methylene blue,Photocatalyst,α-Bi2O3-ZnO,UV-LED,α-Bi2O3
更新于2025-09-11 14:15:04
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Green synthesized CeO <sub/>2</sub> quantum dots: a study of its antimicrobial potential
摘要： Cerium oxide (CeO2) nanoparticles (NPs) of size ～4 nm were successfully synthesized by green synthesis method using cerium nitrate as precursor and aloe-vera leaf extract as reducing and stabilizing agent. The switching between Ce3+ and Ce4+ state by reaction with H2O2 inferred the biomedical applications of CeO2 NPs. This switching enhances due to the presence oxygen vacancies and that was con?rmed by photoluminescence spectrum. The SEM images showed spherical morphology of cubic ?uorite structured CeO2 NPs which avoids the harming of cell. In the present study, CeO2 NPs show signi?cant inhibition effect on gram negative bacteria, E. coli and P. aeruginosa which are responsible for food poisoning. Thus study analyze the synthesized CeO2 NPs as promising candidates for removal of biological pollutants and thus have great potential in the environmental remediation.
关键词： antimicrobial activity,green synthesis,quantum dots
更新于2025-09-11 14:15:04
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Antimicrobial efficacy of methylene blue-mediated photodynamic therapy on titanium alloy surfaces in vitro
摘要： Bacterial elimination using antimicrobial photodynamic therapy (aPDT) has been considered an alternative therapeutic modality in peri-implantitis treatment. The present in vitro study was to evaluate the dose-dependent and pH-dependent bactericidal effects of methylene blue (MB)-mediated aPDT on eliminating Gram-negative (P. gingivalis and A. actinomycetemcomitans) and Gram-positive (S. mutans) bacteria on sandblasting, large-grit and acid-etching (SLA)-pretreated titanium alloy. The effects of different MB concentrations (50, 100, and 200 μg/mL), the pH of the MB (4, 7, and 10), and irradiation time (0, 30, and 60 sec) on the bacterial viability and residual lipopolysaccharide (LPS) levels were examined. The variations in the pH of the MB solution after aPDT for 60 sec on the uncontaminated and contaminated specimens were also detected. The experimental results indicated that MB-mediated PDT could effectively kill the majority of bacteria on the titanium alloy surfaces of biofilm-contaminated implants compared with the MB alone. Of note, aPDT exhibited better antibacterial efficacy with increase in the MB concentration and irradiation time. While treated in an acidic solution on the biofilm-contaminated specimens, aPDT caused the pH to increase. By contrast, the initially high alkaline pH decreased to a value of about pH 8.5 after aPDT. Intriguingly, the neutral pH had minor changes, independent of the MB concentration and bacterial species. As expected, aPDT with higher MB concentration at higher pH environment significantly lowered the LPS concentration of A. actinomycetemcomitans and P. gingivalis. On the basis of the data, the aPDT with 200 μg/mL MB at pH 10 for 60 sec of irradiation time might be an effectively treatment to eliminate bacteria and LPS adherent to titanium surface, however, the use of the multispecies biofilm model and the evaluation of in vitro osteogenesis needed to be further evaluated.
关键词： Peri-implantitis,Antimicrobial activity,Lipopolysaccharide,Methylene blue,Photodynamic therapy
更新于2025-09-10 09:29:36
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Synergistic Photocatalytic-Photothermal Contribution to Antibacterial Activity in BiOI-Graphene Oxide Nanocomposites
摘要： The threat of environmental microbial contamination to the health of human beings has arisen particular attention. In order to explore the synergistic effect of photocatalytic and photothermal process to the antibacterial property, a stably combined BiOI-graphene oxide (GO) nanocomposite was constructed and prepared through a facile solvothermal method. BiOI crystals were uniformly distributed on the GO nanosheets by the formation of Bi-C bond. Based on the various characterizations, the great surface area, the high light harvesting with extension into NIR region and the efficient transfer of photo-induced electrons by the conductivity of GO was demonstrated, which was beneficial for the photocatalytic antibacterial activity. More importantly, the photothermal effect of GO raise the temperature of BiOI-GO composite with high photothermal conversion efficiency and induced the photo-generated electrons from BiOI crystals to obtain more energy and higher carrier mobility. Conversely, the temperature elevation of BiOI-GO composite improved its capability of light absorption and separation of photo-induced charges. As a result, BiOI-GO composite presented the synergistic photocatalytic-photothermal effect to the improvement of antibacterial property for Acinetobacter baumannii with the higher efficiency of TOC removal and leakage of K+ ions, in comparison to the individual photocatalytic process. Thus, the synergistic photocatalytic-photothermal contribution of BiOI-GO composite will provide a significance for the potential application of environmental disinfection in the future.
关键词： Antimicrobial activity,Synergistic photocatalytic-photothermal contribution,BiOI-graphene oxide,Nanocomposite
更新于2025-09-10 09:29:36
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Synthesis, characterisation, and antimicrobial activity of ZnO-based nanocomposites
摘要： In this study, ZnO-based nanocomposites including ZnO/CuO (S1), ZnO/MnO (S2), and ZnO/MnO/CuO (S3), were synthesised. S1, S2, and S3 were characterised through Fourier transform infrared (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS) techniques. Also, the antimicrobial property of the samples was examined against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli through the colony forming count method. FTIR analysis confirmed the presence of Zn–O, Mn–O, and Cu–O in the samples. The crystalline structure of the sample was analysed by XRD. The surface morphology of the prepared compounds was studied with SEM images. EDS technique was employed for ensuring the presence of Zn, Mn, and Cu elements in the samples. The results clearly showed S1, S2, and S3 had high-antimicrobial activity especially for S3.
关键词： SEM,EDS,FTIR,XRD,antimicrobial activity,ZnO-based nanocomposites
更新于2025-09-10 09:29:36
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Solvent Assisted Synthesis of Tin-Zinc Oxide Nanoparticles: Structural Characterization and Antimicrobial Activity
摘要： Tin oxide doped zinc oxide nanoparticles (SnO2/ZnO) were prepared via deposition precipitation method with different molar ratios of tin oxide and effect of variation of concentrations of dopant (SnO2) was observed for physical and chemical properties of these nanoparticle. The structural properties of nanoparticles were characterized by thermogravimetric analysis, fourier transform infrared spectrometry, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy. Their crystallite and structural properties deduced a clear decrement in crystallite size from 9.95 to 2.54 nm. Doping of tin significantly enhanced the photoluminescence and antibacterial properties of SnO2/ZnO nanoparticles. The photoluminescence studies exhibited a strong emission of green-yellow band at 470 nm. The antibacterial activities of these nanoparticles were investigated against Pasteurellamultocida and Escherichia coli by using disc diffusion method.
关键词： Deposition precipitation,SnO2/ZnO,Antimicrobial activity,Photoluminescence
更新于2025-09-09 09:28:46
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Biosynthesis of Silver Nanoparticles Using <i>Selaginella bryopteris</i> Plant Extracts and Studies of Their Antimicrobial and Photocatalytic Activities
摘要： The present study focuses on the green synthesis of silver nanoparticles (AgNPs) using aqueous extract of S. bryopteris which is a lithophyte with remarkable resurrection capabilities. These biosynthesized NPs were characterized with the help of UV-vis spectrophotometer, X-ray diffraction (XRD), FT-IR and Transmission electron microscopy (TEM). Formation of AgNPs was confirmed by UV-Visible spectrophotometer analysis which showed surface plasmon resonance (SPR) at around 420 nm. The TEM images showed the nanoparticles to be polydispersed, nearly spherical in shape and have sizes in range 4–30 nm. The synthesized AgNPs possessed high antibacterial activity as well as photocatalytic dye degradation properties under solar light irradiation in the absence of chemical reducing reagents. Stability of bio-reduced silver nanoparticles was analyzed using UV-vis absorption spectra, and their antimicrobial activity was screened against various gram-positive bacteria.
关键词： Characterization,Photodegradation,Antimicrobial Activity,Biosynthesis,S. bryopteris Extract,Silver Nanoparticles
更新于2025-09-04 15:30:14