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A novel fluorescein-bisphosphonate based diagnostic tool for the detection of hydroxyapatite in both cell and tissue models
摘要： A rapid and efficient method for the detection of hydroxyapatite (HAP) has been developed which shows superiority to existing well-established methods. This fluorescein-bisphosphonate probe is highly selective for HAP over other calcium minerals and is capable of detecting lower levels of calcification in cellular models than either hydrochloric acid-based calcium leaching assays or the Alizarin S stain. The probe has been shown to be effective in both in vitro vascular calcification models and in vitro bone calcification models. Moreover we have demonstrated binding of this probe to vascular calcification in rat aorta and to areas of microcalcification, in human vascular tissue, beyond the resolution of computed tomography in human atherosclerotic plaques. Fluorescein-BP is therefore a highly sensitive and specific imaging probe for the detection of vascular calcification, with the potential to improve not only ex vivo assessments of HAP deposition but also the detection of vascular microcalcification in humans.
关键词： Alizarin S,fluorescein-bisphosphonate,hydroxyapatite,vascular calcification,calcium leaching assays
更新于2025-09-09 09:28:46
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Colorimetric detection of solution concentration based on a paper-based microfluidic device
摘要： A new method for colorimetric detection of solution concentration is presented. A paper-based micro?uidic device was designed and made using printed circuit board technology. Detection zone patterns were designed and transferred to a printed circuit board by hollowing technology. The printed circuit board with patterns was stuck onto a copper sheet. The hollowed zone was ?lled with solid para?n and then covered with a piece of ?lter paper. The para?n wax was melted by heating the copper sheet and permeated into the ?lter paper. Solution to be detected was pipetted on the paper-based device with detective zones. The images of the detective zones were obtained using a microscope with charge-coupled device, which is used for calculating RGBI model to further obtain the solution concentration. Litmus was demonstrated in sample experiments. Results showed that maximum error of measured value of solution concentration to its true value is less than 2% using the presented method.
关键词： concentration,detective zone,Paper-based device,colorimetric assays
更新于2025-09-09 09:28:46