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Carbon dots as a dual sensor for the selective determination of d-penicillamine and biological applications
摘要： Dual mode Nanosensors gained tremendous interest in recent years because of their facile synthesis and dual applications. Herein we have synthesized highly fluorescent carbon dots (CDs) from Mahogany fruit shell by chemical oxidation method. The as prepared CDs exhibited selective and sensitive quenching of fluorescence by Fe3+ and also accompanied by a dramatic increase in absorption intensity. Hence these two processes led to fabricate CDs-Fe3+ system as a dual probe. D-Penicillamine (D-PA) has much affinity toward Fe3+ which resulted in recovery of almost 75% fluorescence intensity and decrease in absorption of the CDs-Fe3+ system. Thus, this tendency has been exploited for the selective detection of D-PA by both Spectrofluorimetrically and UV-Visible spectroscopically and showed a wide linear range of detection 0-48 μg mL?1 respectively. This developed probe offered low cost, high selectivity, repeatability, facile operation and excellent recovery ratio in detection of D-PA in pharmaceutical samples. Moreover, biological applications CDs were investigated using Saccharomyces cerevisiae strain with confocal microscopy. We found that CDs were a biocompatible and ideal candidate for differential staining of yeast cells.
关键词： D-penicillamine detection,Cell imaging,Carbon dots (CDs),Dual sensor,Biocompatibility,UV-Visible and spectrofluorimetrically
更新于2025-09-10 09:29:36
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High-fluorescent carbon dots (CDs) originated from China grass carp scales (CGCS) for effective detection of Hg(II) ions
摘要： Carbon dots (CDs) as a kind of environment-friendly fluorescent nanomaterial has been actively studied because of wide and potential applications, such as bioimaging and biosensors. It is worth mentioning that preparing CDs from the recycling of carbonaceous waste has received considerable research interest. We proposed a simple carbon dots synthesis technique, that is, using China grass carp scales (CGCS) as original and green materials by a one-step microwave hydrothermal method to prepare CDs. Since the mercury ion has strongly sulphophile, S atoms display higher thermodynamic affinity and faster integrating process with Hg2+ ions. Due to the presence of large amount of cysteine-containing sulfhydryl groups, the obtained CGCS-CDs can be used as a specific fluorescence probe for detecting Hg2+ ions. Subsequently, CGCS-CDs were characterized by HR-TEM, XRD, FT-IR and XPS analyses. The optical properties of CGCS-CDs were elucidated by fluorescence and UV–vis spectra. Additionally, the effects of pH values and salt concentrations on optical properties of CGCS-CDs were also researched by fluorescence spectra. Under optimal conditions, the decrease of fluorescence intensity displays a good linear relationship with allowable Hg2+ ion detection concentration range of 0.014–30 μmol/L and a limit of detection (LOD) of 0.014 μmol/L is acquired. Meanwhile, the effect of other metal ions on the detection of Hg2+ ions by using CGCS-CDs as fluorescence probe was studied, suggesting that the CGCS-CDs could be an excellent fluorescence probe for the detection of Hg2+ ions. Eventually, the CGCS-CDs were proven to be low toxicity and applied for Hg2+ ions detection in lake water and cosmetic, demonstrating their potential towards diverse applications.
关键词： Sulfhydryl group,Microwave hydrothermal,Nanoscale sensing,Mercury,Carbon dots (CDs),China grass carp scales (CGCS)
更新于2025-09-09 09:28:46