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UV-visible scanning spectrophotometry and chemometric analysis as tools for carotenoids analysis in cassava genotypes (Manihot esculenta Crantz)
摘要： In this study, the metabolomics characterization focusing on the carotenoid composition of ten cassava (Manihot esculenta) genotypes cultivated in southern Brazil by UV-visible scanning spectrophotometry and reverse phase-high performance liquid chromatography was performed. Cassava roots rich in β-carotene are an important staple food for populations with risk of vitamin A deficiency. Cassava genotypes with high pro-vitamin A activity have been identified as a strategy to reduce the prevalence of deficiency of this vitamin. The data set was used for the construction of a descriptive model by chemometric analysis. The genotypes of yellow-fleshed roots were clustered by the higher concentrations of cis-β-carotene and lutein. Inversely, cream-fleshed roots genotypes were grouped precisely due to their lower concentrations of these pigments, as samples rich in lycopene (red-fleshed) differed among the studied genotypes. The analytical approach (UV-Vis, HPLC, and chemometrics) used showed to be efficient for understanding the chemodiversity of cassava genotypes, allowing to classify them according to important features for human health and nutrition.
关键词： Chemometrics,cassava genotypes,partial metabolome,descriptive models,UV-vis,carotenoids,RP-HPLC
更新于2025-09-23 15:22:29
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Application of infrared thermography to assess cassava physiology under water deficit condition
摘要： Water deficit stress is a major factor that inhibits the overall growth and development in cassava (Manihot esculenta), leading to decreased storage root yield. We conducted a study to investigate whether thermal sensing could be used to indicate water deficit stress and the health and yield of cassava crops in field. The objective of the study was to use thermal imaging to determine relationship between crop water stress index (CWSI) and physiological changes, and to identify the critical CWSI point in fields of cassava cv. Rayong 9 under well-irrigated and water-deficit conditions. At the time of storage root initiation (85 DAP [day after planting]), thermal imagery was collected and the physiological changes and growth characters were measured prior to storage root harvesting (162 DAP). Thermal infrared imager was used to measure the canopy temperature and CWSI of cassava plants. Net photosynthetic rate (Pn), stomatal conductance (gs) and transpiration rates (Tr) of cassava plants under water deficit conditions for 29 d (114 DAP) were significantly decreased, leading to delayed plant growth as compared to those under well-irrigated conditions. In contrast, air vapor pressure deficit (VPDair) and CWSI in drought-stressed plants were higher than well irrigated plants. High correlations between Tr/gs/Pn and CWSI were observed. The study concludes that CWSI is a sensitive indicator of water deficit stress caused due to stomatal function.
关键词： net photosynthetic rate,crop water stress index,thermal imagery,Cassava,stomatal conductance
更新于2025-09-23 15:21:01
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Trichromophore-doped cassava-based biopolymer as low-cost and eco-friendly luminous material for bio hybrid white-light-emitting diodes by dual-FRET process
摘要： White-light-emitting diodes (WLEDs) are promoted as environmentally friendly because they are energy saving-features and mercury-free aspects, but, rare-earth-free, metal-free, cost-competitive, and eco-friendly for brighter WLEDs are attractive but quite challenging in practice. This paper reports a new methodology using biopolymer luminous material in bio hybrid white-light-emitting diodes (Bio-HWLEDs) based on a trichromophore-doped cassava crystalline thick film that emits bright white light emission through a dual F?rster resonance energy transfer (FRET) process. The dual-FRET takes place from coumarin to sulforhodamine via the curcumin chromophore. The Bio-HWLED showed color co-ordinates (0.33, 0.32) that exactly matched with pure white light emission. The effect of temperature on luminescence and the activation energy for thermal quenching were determined using the temperature-dependent photoluminescence measurements. Moreover, Bio-HWLED showed a low luminous drop rate of 0.00069 s?1 to overcome the aggregation-induced quenching effect. These results pave the way towards the realization of commercially viable, large-scale and high-contrast bio hybrid light applications that are environmentally friendly.
关键词： Chromophore,Cassava,Bio-LEDs,Dual-FRET,Quenching effect
更新于2025-09-16 10:30:52