- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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A ratiometric fluorescence probe based on a novel recognition mechanism for monitoring endogenous hypochlorite in living cells
摘要： A ratiometric fluorescence probe (named ZOC) for the fast detection of HClO/ClO- was constructed by coumarin (donor) and pyridinium (acceptor) based on Forster resonance energy transfer (FRET) and intramolecular charge transfer (ICT) platform. ZOC possessed red emission signal (610 nm), large Stocks shift (190 nm), high energy transfer efficiency (95.3%), high selectivity and sensitivity, low detection limit (25 nM), wider detection range (from 25 nM to 30 μM), rapid response (within 13 S), and good biocompatibility. It was very interesting that the recognition mechanism involved a new organic reaction in which olefin double bond reacted first with HClO/ClO- regioselectively, followed by cyclization. ZOC was successfully used to the real time detection of endogenous HClO/ClO- in RAW 264.7 cells.
关键词： Hypochlorous acid/hypochlorite,Probe,Cell image,Ratiometric fluorescence,New mechanism
更新于2025-11-21 11:08:12
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A new FRET-based ratiometric fluorescence probe for hypochlorous acid and its imaging in living cells
摘要： A novel ratiometric fluorescence probe for hypochlorous acid was constructed by coumarin and pyridinium fluorophore based on the Forster resonance energy transfer (FRET) and intramolecular charge transfer (ICT) platform. In this ICT/FRET system, the energy transfer efficiency is high to 94.3%. Moreover, the probe could respond to hypochlorous acid with high selectivity and sensitivity, and exhibited a large Stokes shift. It was interesting to find that the probe could recognize hypochlorous acid via a new mechanism, in which the a -position of carbonyl group was oxidized to form a diketone derivative. More importantly, the probe was successfully applied to the ratiometric imaging of both exogenous and endogenous hypochlorous acid in living RAW 264.7 cells, with low toxicity and high photo-stability.
关键词： New reaction mechanism,FRET,Hypochlorous acid,Fluorescence probe,Cell image,Ratiometric
更新于2025-11-21 11:08:12
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[IEEE 2018 IEEE 3rd International Conference on Image, Vision and Computing (ICIVC) - Chongqing (2018.6.27-2018.6.29)] 2018 IEEE 3rd International Conference on Image, Vision and Computing (ICIVC) - Research on Single-Frame Super-Resolution Reconstruction Algorithm for Low Resolution Cell Images Based on Convolutional Neural Network
摘要： At the problem of low resolution and low contrast of cell images collected by lens-less cell imaging system, a novel cell super-resolution reconstruction network (CSRN) based on convolutional neural network is proposed. First, the cell image is collected by lens-less cell imaging system, and then the cell image is down-sampled by bicubic interpolation to obtain low-resolution cell image. Then, the low-resolution cell image is input into the CSRN network for super-resolution reconstruction. The experimental results show that the proposed CSRN network can effectively improve the resolution and contrast of cell images, and the reconstruction effect is better than that of the traditional bicubic interpolation and SRCNN network.
关键词： convolutional neural network,cell image,super-resolution,lens-less imaging
更新于2025-09-23 15:23:52
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[IEEE 2018 IEEE International Conference on Imaging Systems and Techniques (IST) - Krakow (2018.10.16-2018.10.18)] 2018 IEEE International Conference on Imaging Systems and Techniques (IST) - The analysis of the movement of the genetically modified human skin fibroblasts in culture
摘要： The aim of this study is the comparison of the movement of various types of human dermal fibroblasts with and without genetic modification. Three groups of fibroblasts were cultured and monitored: (1) control group of fibroblasts isolated from human skin (not transduced); (2) transduced with lentivirus bearing EGFP fluorescent marker; (3) transduced with lentivirus bearing DsRed2 fluorescent marker. The experimental sequences of images documenting cells’ movement have been analyzed using image processing methods. The cells’ movement was described by: (1) distance between each two consecutive images/frames, (2) distance and displacement covered by each fibroblast in 30 minutes (3) length of cell crawling cycle, (4) the movement tortuosity coefficient for all fibroblasts in each group. Also shape features such as: area, perimeter, eccentricity, length of the major axis have been analyzed. It appears that the general movement behavior is not changed by the process of transduction but some of its aspects are modified. The efficiency of movement in the sense of distance covered and region penetration is decreased because of cells’ morphology. Transduced cells are less polarized and develop extra podia during their crawling.
关键词： EGFP modified cell,DsRed2 modified cell,genetically modified cell,image analysis,quantitative analysis of cells’ movement,quantitative analysis of shape,human skin fibroblast
更新于2025-09-23 15:22:29
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CdS quantum dots embedded in PVP: Inorganic phosphate ion sensing in real sample and its antimicrobial activity
摘要： Polyvinyl-pyrrolidone capped spherical cadmium sulphide quantum dots (CdS-PVP QDs), 2–6 nm in size, were developed as a selective turn-on fluorescence nanosensor for monohydrogen phosphate ion (HPO4 2?) in aqueous medium. Fluorescence intensity of CdS-PVP QDs significantly increased with addition of HPO4 2? ions, whereas the other common inorganic ions had very little effect on the fluorescence intensity. The proposed sensor may be efficiently used for the detection of HPO4 2? ions at a low level of concentration up to 213 nM in real urine sample. Cell imaging study indicates that the CdS-PVP QDs are cell permeable and can detect the intracellular distribution of HPO4 2? ions under fluorescence microscope. The CdS-PVP QDs showed considerable activity against Staphylococcus aureus also.
关键词： Chemosensor,CdS,Antimicrobial activity,Cell image,Quantum dots,Phosphate ion sensing
更新于2025-09-19 17:13:59