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A novel photosensitive dual-sensor for simultaneous detection of nucleic acids and small chemical molecules
摘要： Sensors that can rapidly and specifically detect nucleic acids and chemical molecules can revolutionize the diagnosis and treatment of diseases by allowing molecular-level informations to be used during the routine medicines. In this study, we demonstrated a novel dual-sensor that can be used to simultaneously detect any nucleic acids and chemical molecules whose binding aptamers can be found or synthesized. In the developed dual-sensor, the specifically designed PTG (a photosensitive azobenzene derivative carrying one photo-isomerizable azobenzene moiety, one threoninol terminal and one guanidinium terminal) molecules are introduced into the unwinding region of two T7 promoters, and two DNA bubbles are introduced upstream of the two T7 promoters. Without the target, the indicating gene in the dual-tensor would not be expressed since the binding with RNAPs (RNA polymerases) cannot melt the T7 promoter for the indicating gene due to the integration of the DNA double strands via the PTG molecules, manifesting the absence of the target nucleic acid and chemical molecule. While with the presence of the target nucleic acid and/or chemical molecule, the indicating gene would be expressed as the T7 promoter contained in the enlarged DNA bubble can be melted and transcribed by the bound RNAPs as the enlarged DNA bubble can help the separation of the two DNA strands, demonstrating the existence of target nucleic acid and/or chemical molecule.
关键词： Nucleic acids,DNA melting,Gene expression,DNA nanotechnology,Dual-sensor
更新于2025-09-23 15:23:52
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Carbon dots as a dual sensor for the selective determination of d-penicillamine and biological applications
摘要： Dual mode Nanosensors gained tremendous interest in recent years because of their facile synthesis and dual applications. Herein we have synthesized highly fluorescent carbon dots (CDs) from Mahogany fruit shell by chemical oxidation method. The as prepared CDs exhibited selective and sensitive quenching of fluorescence by Fe3+ and also accompanied by a dramatic increase in absorption intensity. Hence these two processes led to fabricate CDs-Fe3+ system as a dual probe. D-Penicillamine (D-PA) has much affinity toward Fe3+ which resulted in recovery of almost 75% fluorescence intensity and decrease in absorption of the CDs-Fe3+ system. Thus, this tendency has been exploited for the selective detection of D-PA by both Spectrofluorimetrically and UV-Visible spectroscopically and showed a wide linear range of detection 0-48 μg mL?1 respectively. This developed probe offered low cost, high selectivity, repeatability, facile operation and excellent recovery ratio in detection of D-PA in pharmaceutical samples. Moreover, biological applications CDs were investigated using Saccharomyces cerevisiae strain with confocal microscopy. We found that CDs were a biocompatible and ideal candidate for differential staining of yeast cells.
关键词： D-penicillamine detection,Cell imaging,Carbon dots (CDs),Dual sensor,Biocompatibility,UV-Visible and spectrofluorimetrically
更新于2025-09-10 09:29:36