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Selective and Sensitive Sensing of Hydrogen Peroxide by a Boronic Acid Functionalized Metal–Organic Framework and Its Application in Live-Cell Imaging
摘要： A new boronic acid functionalized Zr(IV) metal?organic framework having the capability of sensing H2O2 in live cells is reported. The Zr-MOF bears a UiO-66 structure and contains 2-boronobenzene-1,4-dicarboxylic acid (BDC-B(OH)2) as a framework linker. The activated Zr-UiO-66-B(OH)2 compound (called 1′) is highly selective for the ?uorogenic detection of H2O2 in HEPES bu?er at pH 7.4, even in the presence of interfering ROS (ROS = reactive oxygen species) and other biologically relevant analytes. The ?uorescent probe was found to display extraordinary sensitivity for H2O2 (detection limit 0.015 μM) in HEPES bu?er, which represents a lower value in comparison to those of the MOF probes documented so far for sensing H2O2 using other analytical methods. Taking advantage of its high selectivity and sensitivity for H2O2 in HEPES bu?er, the probe was successfully employed for the imaging of intracellular H2O2. Imaging studies with MDAMB-231 cells revealed the emergence of bright blue ?uorescence after loading with probe 1′ and subsequent treatment with H2O2 solution.
关键词： Zr(IV) metal?organic framework,boronic acid,live-cell imaging,fluorescent probe,H2O2 sensing
更新于2025-09-23 15:21:21
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Hemoglobin Immobilization on Multiporous Nanofibers of SnO <sub/>2</sub> and Chitosan Composite for Hydrogen Peroxide Sensing
摘要： A multiporous nanofiber (MPNFs) of SnO2 and chitosan has been used for the immobilization of a redox protein, hemoglobin (Hb), onto the surface of glassy carbon electrode (GCE). The multiporous nanofiber of SnO2 that has very high surface area is synthesized by using electrospinning technique through controlling the tin precursor concentration. Since the constructed MPNFs of SnO2 exposes very high surface area, it increases the efficiency for biomolecule-loading. The morphology of fabricated electrodes is examined by SEM observation and the absorbance spectra of Hb/(MPNFs) of SnO2 are studied by UV-Vis analysis. Cyclic Voltammetry and amperometry are employed to study and optimize the performance of the resulting fabricated electrode. After fabrication of the electrode with the Hb and MPNFs of SnO2, a direct electron transfer between the protein’s redox centre and the glassy carbon electrode was established. The modified electrode has showed a couple of redox peak located at ?0.29 V and ?0.18 V and found to be sensitive to H2O2. The fabricated electrode also exhibited an excellent electrocatalytic activity towards the reduction of H2O2. The catalysis currents increased linearly to the H2O2 concentration in a wide range of 5.0 × 10?6–1.5 × 10?4 M. Overall experimental results show that MPNFs of SnO2 has a role towards the enhancement of the electroactivity of Hb at the electrode surface. Thus the MPNFs of SnO2 is a very promising candidate for future biosensor applications.
关键词： Multiporous SnO2 Nanofiber,Hemoglobin,Electrical Contact,H2O2 Sensing,Direct Electrochemistry
更新于2025-09-09 09:28:46