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FRET-based dual channel fluorescent probe for detecting endogenous/exogenous H2O2/H2S formation through multicolor images
摘要： We have developed a FRET-based fluorescent probe (PHS1) as a combination of two different fluorophores (coumarin and naphthalimide); which can detect both exogenous and endogenous H2S and H2O2 in live cells through multicolor images. The precise overlap between UV-absorption of naphthalimide and the emission band of coumarin in probe PHS1 allows the acquisition of the self-calibrated information of dual analytes through FRET-based imaging. The UV–Vis absorption (λabs 390 nm) and fluorescence emission (λem 460 nm) of probe PHS1 in the presence of H2O2 are increased ∽35- fold and ∽15-fold respectively. It also allows the estimation of the levels of H2S through enhancement of emission intensity at 550 nm. The probe PHS1 exhibits high stability against various analytes, including various pH (4–9.5). The cell viability assay data indicate that the probe is not harmful to the cancer cells. The nontoxic nature of the probe PHS1 encourages application for cancer cell labeling. The probe PHS1 can detect the level of endogenous H2O2, H2S, and H2O2/H2S in cancer cells through blue, green and FRET-based green channel imaging. PHS1 is a unique probe, has potential application for diagnosing cancer by providing information on the level of dual analytes (H2S, H2O2) in cancer cells.
关键词： FRET-based fluorescent probe,Naphthalimide,Endogenous H2O2,Endogenous H2S,Coumarin
更新于2025-11-19 16:46:39
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A coumarin-based colorimetric fluorescent probe for rapid response and highly sensitive detection of hydrogen sulfide in living cells
摘要： Hydrogen sulfide (H2S) plays a vital role in numerous biological processes in living organisms. To better understand its functions, a fluorescent probe to fast and sensitively detect H2S is imminently needed. Keep this in mind, we reasonably designed probe DHC for detecting H2S based on α, β-unsaturated ethanoylcoumarin fluorophore. The limit of detection (LOD) is found to be as low as 5 × 10?8 M, which is superior to most reported fluorescent probes to detect H2S. Furthermore, the wide pH range of 4–11 makes it capable of application in biological systems. Most importantly, MTT assays and cell imaging experiments indicate that probe DHC has hypotoxicity and outstanding membrane permeability, which makes DHC successful imaging of H2S in Baby Hamster Syrian Kidney (BHK) cells.
关键词： Cell-imaging,Fluorescent probe,Rapid response,H2S,Coumarin
更新于2025-09-23 15:23:52
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Synthesis of Cu2O/CuO Nanocrystals and Their Application to H2S Sensing
摘要： Semiconducting metal oxide nanocrystals are an important class of materials that have versatile applications because of their useful properties and high stability. Here, we developed a simple route to synthesize nanocrystals (NCs) of copper oxides such as Cu2O and CuO using a hot-soap method, and applied them to H2S sensing. Cu2O NCs were synthesized by simply heating a copper precursor in oleylamine in the presence of diol at 160 °C under an Ar flow. X-ray diffractometry (XRD), dynamic light scattering (DLS), and transmission electron microscopy (TEM) results indicated the formation of monodispersed Cu2O NCs having approximately 5 nm in crystallite size and 12 nm in colloidal size. The conversion of the Cu2O NCs to CuO NCs was undertaken by straightforward air oxidation at room temperature, as confirmed by XRD and UV-vis analyses. A thin film Cu2O NC sensor fabricated by spin coating showed responses to H2S in dilute concentrations (1–8 ppm) at 50–150 °C, but the stability was poor because of the formation of metallic Cu2S in a H2S atmosphere. We found that Pd loading improved the stability of the sensor response. The Pd-loaded Cu2O NC sensor exhibited reproducible responses to H2S at 200 °C. Based on the gas sensing mechanism, it is suggested that Pd loading facilitates the reaction of adsorbed oxygen with H2S and suppresses the irreversible formation of Cu2S.
关键词： CuO,H2S,gas sensor,Cu2O,nanocrystal
更新于2025-09-23 15:22:29
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Remarkable reproducibility and significant sensitivity of ZnO nanoparticles covered by Chromium (III) oxide as a hydrogen sulfide gas sensor
摘要： ZnO nanoparticles covered by Chromium (III) oxide (Cr2O3) were fabricated using spray pyrolysis and thermal evaporation technique to increase the H2S gas sensor repeatability. Gas sensing analyses showed that the Cr2O3 cover on the ZnO nanoparticle increased the response at high concentrations without affecting the operating temperature. Furthermore, there was no change in the response and recovery time after coating Cr2O3. ZnO nanoparticles covered by Cr2O3 demonstrated considerable reproducibility.
关键词： H2S,Cr2O3,Sensor,ZnO,sensitivity.,longevity
更新于2025-09-23 15:22:29
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One-step H2S reactive sputtering for 2D MoS2/Si heterojunction photodetector
摘要： A technique for directly growing two-dimensional (2D) materials onto conventional semiconductor substrates, enabling high-throughput and large-area capability, is required to realise competitive 2D transition metal dichalcogenide devices. A reactive sputtering method based on H2S gas molecules and sequential in-situ post-annealing treatment in the same chamber was proposed to compensate for the relatively deficient sulfur atoms in the sputtering of MoS2 and then applied to a 2D MoS2/p-Si heterojunction photodevice. X-ray photoelectron, Raman, and UV-visible spectroscopy analysis of the as-deposited Ar/H2S MoS2 film were performed, indicating that the stoichiometry and quality of the as-deposited MoS2 can be further improved compared with the Ar-only MoS2 sputtering method. For example, Ar/H2S MoS2 photodiode has lower defect densities than that of Ar MoS2. We also determined that the factors affecting photodetector performance can be optimised in the 8–12 nm deposited thickness range.
关键词： H2S gas,two-dimensional layered MoS2,reactive sputtering,heterojunction photodiode
更新于2025-09-23 15:19:57
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Ultrathin Hexagonal PbO Nanosheets Induced by Laser Ablation in Water for Chemically Trapping Surface-Enhanced Raman Spectroscopy Chips and Detection of Trace Gaseous H <sub/>2</sub> S
摘要： Lead oxide (PbO) nanosheets are of significance in design of functional devices. However, facile, green and fast fabrication of ultrathin and homogenous PbO nanosheets with chemically clean surface is still desirable. Herein, a simple and chemically clean route is developed for fabricating such nanosheets via laser ablation of a lead target in water for a short time and then ambient ageing. The obtained PbO nanosheets are (002)-oriented with microsize in planar dimension and ca. 15 nm in thickness. They are mostly hexagonal in shape. Experimental observations of the morphological evolution have revealed that the formation of such PbO nanosheets can be attributed to two processes: (i) laser ablation-induced formation of ultrafine Pb and PbO nanoparticles (NPs); (ii) PbO NPs’ aggregation and their oriented connection growth. Importantly, a composite surface enhanced Raman spectroscopy (SERS) chip is designed and fabricated, by covering a PbO nanosheets’ monolayer on a Au NPs’ film. Such composite SERS chip can be used for the fast and trace detection of gaseous H2S, in which the PbO nanosheets can effectively chemically trap H2S molecules, demonstrating a new application of these PbO nanosheets. The response of this chip to H2S can be detected within 10s and the detection limit is below 1 ppb. Also, this PbO nanosheet-based chip is reusable by heating after use. This study not only deepens understanding the NPs-based formation mechanism of nanosheets, but also provides the renewable SERS-chips for the highly efficient detection of trace gaseous H2S.
关键词： Ultrathin hexagonal PbO nanosheets,Chemically trapping-SERS chip,Trace detection of gaseous H2S,Reusable performance,Laser ablation in water
更新于2025-09-23 15:19:57
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The Catalytic Activity Enhancement of Commercial TiO2 and Nb2O5 Catalysts by Iron for Elemental Sulfur Production from H2S
摘要： Commercial TiO2 and Nb2O5 catalysts were used to determine catalytic activities for selective oxidation of H2S to elemental sulfur. Fe@TiO2 and Fe@Nb2O5 catalysts (containing 10% iron by weight) were also prepared by wet impregnation method to enhance the catalytic activity. TiO2 anatase phase and Nb2O5 were mainly observed in the crystalline structure of TiO2 and Nb2O5 based catalysts, respectively. Catalytic activity tests were performed in a fixed-bed flow reactor at 250 °C using stoichiometric feed ratio. 30% and 28% H2S conversions were obtained with commercial TiO2 and Nb2O5 catalysts. Complete conversion of H2S was reached with Fe@TiO2 and Fe@Nb2O5 catalysts at the same reaction conditions for 400 min. of reaction time. 100% of H2S conversion was obtained with iron-containing catalysts in the reaction tests carried out at 200 °C and 300 °C of reaction temperatures. Fe@TiO2 and Fe@Nb2O5 catalysts showed high sulfur selectivity (≥ 95%) under all reaction conditions. Iron addition enhanced the Lewis acidity and redox property of the commercial catalysts and this may be the reason for increase in catalytic activity.
关键词： H2S,Elemental sulfur,Commercial catalyst,Iron
更新于2025-09-23 15:19:57
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Ultra-Sensitive H<sub>2</sub>S Gas Sensor Based on WO<sub>3</sub> Nanocubes with Low Operating Temperature
摘要： WO3 nanostructure with nanocube morphology was synthesized through acidification of Na2WO4·2H2O, which were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Moreover, the result of the present work implied that the sensor fabricated by nanocube WO3 could detect the level of 330 ppb H2S, which is much lower than the threshold limit value of 10 ppm. Compared with other results, the nanocube WO3 sensor shows higher sensitivity, excellent selectivity and faster response/recovery to H2S. Especially, the best operating temperature of this nanocube WO3 for H2S detection is 100 oC.
关键词： H2S,Gas sensor,Nanomaterials,WO3
更新于2025-09-19 17:15:36
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Imaging of anti-inflammatory effects of HNO <i>via</i> a near-infrared fluorescent probe in cells and in rat gouty arthritis model
摘要： Nitroxyl (HNO) plays a crucial role in anti-inflammatory effects via the inhibition of inflammatory pathways, but the details of the endogenous generation of HNO still remain challenging owing to the complex biosynthetic pathways, in which the interaction between H2S and NO simultaneously generates HNO and polysulfides (H2Sn) in mitochondria. Moreover, nearly all the available fluorescent probes for HNO are utilized for imaging HNO in cells and tissues, instead of the in situ real-time detection of the simultaneous formation of HNO and H2Sn in mitochondria and animals. Here, we have developed a mitochondria-targeting near-infrared fluorescent probe, namely, Mito-JN, to detect the generation of HNO in cells and a rat model. The probe consists of three moieties: Aza-BODIPY as a fluorescent signal transducer, a triphenylphosphonium cation as a mitochondria-targeting agent, and a diphenylphosphinobenzoyl group as an HNO-responsive unit. The response mechanism is based on an aza-ylide intramolecular ester aminolysis reaction with fluorescence emissions on. Mito-JN displays high selectivity and sensitivity for HNO over various other biologically relevant species. Mito-JN was successfully used for the detection of the endogenous generation of HNO, which is derived from the crosstalk between H2S and NO in living cells. The additional generation of H2Sn was also confirmed using our previous probe Cy-Mito. The anti-inflammatory effect of HNO was examined in a cell model of LPS-induced inflammation and a rat model of gouty arthritis. The results imply that our probe is a good candidate for the assessment of the protective effects of HNO in inflammatory processes.
关键词： H2S,fluorescent probe,HNO,polysulfides,gouty arthritis,anti-inflammatory,near-infrared,NO,mitochondria-targeting
更新于2025-09-19 17:15:36
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A highly selective and sensitive fluorescent probe for simultaneously distinguishing and sequentially detecting H <sub/>2</sub> S and various thiol species in solution and in live cells
摘要： A novel dual-channel fluorescent probe (NCR) based on differences in reactivity among H2S, Cys/Hcy, and GSH was rationally designed for simultaneously distinguishing and sequentially sensing H2S, Cys/Hcy, and GSH using two emission channels, which also demonstrated that NCR can be used for targeting mitochondria in mammalian cells.
关键词： live cells,thiol species,fluorescent probe,mitochondria-targeted,H2S
更新于2025-09-19 17:15:36