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Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide
摘要： ● AIM: To explore the effect of ciliary neurotrophic factor (CNTF) on retinal ganglion cell (RGC)-5 induced by hydrogen peroxide (H2O2). ● METHODS: After cell adherence, RGC-5 culture medium was changed to contain different concentrations of H2O2 from 50 to 150 μmol/L at four time points (0.5, 1, 1.5 and 2h) to select the concentration and time point for H2O2 induced model. Two different ways of interventions for injured RGC-5 cells respectively were CNTF as an addition in the culture medium or recombinant lentiviral plasmid carrying CNTF gene transfecting bone mesenchymal stem cells (BMSCs) for co-culture with RGC-5. ● RESULTS: Compared to the control group, H2O2 led to RGC-5 death closely associated with concentrations and action time of H2O2 and we chose 125 μmol/L and 2h to establish the H2O2-induced model. While CNTF inhibited the loss of RGC-5 cells obviously with a dose-dependent survival rate. Nevertheless two administration routes had different survival rate yet higher rate in recombinant lentiviral plasmid group but there were no statistically significant differences. ● CONCLUSION: Both the two administration routes of CNTF have effects on RGC-5 cells induced by H2O2. If their own advantages were combined, there may be a better administration route.
关键词： retinal ganglion cells,ciliary neurotrophic factor,neuroprotection,hydrogen peroxide,recombinant lentiviral vector
更新于2025-09-04 15:30:14
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[IEEE 2018 IEEE International Conference on Semiconductor Electronics (ICSE) - Kuala Lumpur (2018.8.15-2018.8.17)] 2018 IEEE International Conference on Semiconductor Electronics (ICSE) - Detecting Hydrogen Using TiO<inf>2</inf>-B<inf>2</inf>O<inf>3</inf> at Different Operating Temperature
摘要： Performance of TiO2-B2O3 gas sensor that annealed using nitrogen at 650°C for 30 minutes was observed and analyzed. The sensing film of the gas sensor was prepared by mixing TiO2-B2O3 with an organic binder. The sensing film was characterized by field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD). The gas sensor was exposed to hydrogen at a concentration of 100 - 1000 ppm with operating temperatures of 100°C and 200°C. However, no response was detected for 100 ppm at 100°C. But, as the operating temperature was increased to 200°C, the gas sensor indicated a good response for 100 ppm of hydrogen. The gas sensor exhibited p-type response based on decreased current when exposed to hydrogen. The sensitivity of gas sensor was calculated at 1.00, 2.18 and 3.58 for 100 ppm, 500 ppm and 1000 ppm respectively, at an operating temperature of 200°C.
关键词： TiO2-B2O3,hydrogen,organic binder,nitrogen,gas sensor
更新于2025-09-04 15:30:14
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A Highly Fluorescent and Photoresponsive Polymer Gel Consisting of Poly(acrylic acid) and Supramolecular Cyanostilbene Crosslinkers
摘要： A poly(acrylic acid)-based polymer gel exhibiting strong fluorescence and high photoresponsibility is prepared by using a cyanostilbene derivative as a self-assembling supramolecular crosslinking agent, which generates conceptually new multiple hydrogen bonds in the gel system. This polymer gel in ethanol shows the gel-to-sol transition upon UV light irradiation with concomitant fluorescence color and intensity changes, which are attributed to the trans-to-cis photoisomerization of the supramolecular crosslinkers. Aggregation-induced enhanced emission (AIEE) behavior of the trans-cyanostilbene derivative enables a highly fluorescent polymer gel (ΦF = 0.68) with green color (λem = 541 nm), which can be switched into a blue fluorescent sol (λem = 506 nm and ΦF = 0.12) upon UV irradiation.
关键词： polymer gels,fluorescence switching,photoisomerization,gel-to-sol transition,hydrogen bonding
更新于2025-09-04 15:30:14