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Strength in Numbers: Development of a Fluorescence Sensor Array for Secondary Structures of DNA
摘要： High-throughput assessment of secondary structures adopted by DNA oligonucleotides is currently hampered by the lack of suitable biophysical methods. Fluorescent sensors hold great potential in this respect; however, the moderate selectivity that they display for one DNA conformation over the others constitutes a major drawback to the development of accurate assays. Moreover, the use of single sensors impedes a comprehensive classification of the tested sequences. Herein, we propose to overcome these limitations through the development of a fluorescence sensor array constituted by easily accessible, commercial dyes. Multivariate analysis of the emission data matrix produced by the array allows to explore the conformational preferences of DNA sequences of interest, either by calculating the probability of group membership in the six predefined structural categories (three G-quadruplex groups, double-stranded, and two groups of single-stranded forms), or by revealing their particular structural features. The assay enables rapid screening of synthetic DNA oligonucleotides in a 96-well plate format.
关键词： fluorescence sensor array,G-quadruplex,high-throughput conformational analysis,multivariate analysis,DNA secondary structures
更新于2025-09-09 09:28:46
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Assembly of a miRNA-modified QCM sensor for miRNA recognition through response patterns
摘要： In this paper, a miRNA‐based quartz crystal microbalance (QCM) biosensor was fabricated and used to the rapid and effective sensing of miRNA. The specific hybridization between probe miRNA and different selected miRNAs (miR‐27a, miR‐27b, and Let‐7a) cause a different interaction mode, thus display different frequency change and response patterns in the QCM sensor, which were used to detect miR‐27a and miR‐27b. The selective sensing of miR‐27a in mixed miRNA solution was also achieved. This miRNA‐based QCM biosensor has the advantages of real‐time, label‐free, and short cycle detection.
关键词： miR‐27b,miRNA,miR‐27a,response pattern,QCM sensor
更新于2025-09-09 09:28:46
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NTD-Ge Production in the LUMINEU Experiment Using Cryogenic Detectors for Rare Event Searches and Other Applications
摘要： To cope with the future large demand on neutron-transmutation-doped germanium of (NTD) sensors, our groups in LUMINEU started a new production line for NTD-Ge. This paper contains details on the NTD-Ge production, resistance measurement and a comparison of the performance in terms of noise and signal-to-noise ratio with an existing sensor contacts. This demonstrates our ability to produce NTD sensors for the desired range of working temperature.
关键词： Thermal sensor,Dark matter,Cryogenic,Double beta decay
更新于2025-09-09 09:28:46
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Molecularly imprinted photoelectrochemical sensor for fumonisin B1 based on GO-CdS heterojunction
摘要： A rapid and ultrasensitive molecularly imprinted photoelectrochemical (MIP-PEC) sensing platform based on ITO electrode modified with GO-CdS heterojunction was prepared for ultrasensitive measure of fumonisin B1 (FB1). CdS quantum dots (QDs) were combined with a suitable amount of graphene oxide (GO) to form a heterojunction to enhance signal response with accurately calculating energy levels (VB/CB or HOMO/LUMO). The MIP-PEC sensor was successful fabricated after MIP was immobilized on the electrode with the basis of these results. In the phosphate buffer solution (PBS), it was clearly observed that the non-elution MIP-PEC sensor had almost no photocurrent response, which was due to the slower electron transfer speed. When the MIP-PEC sensor is eluted in ethanol, its photocurrent response was significantly restored, that was because the fact that the template molecules were washed away, and electron donors entered the holes and accelerated the electron transfer. Its photocurrent response was reduced because of holes blocked when the MIP-PEC sensor was hatched in the template molecules culture fluid. This phenomenon fully showed that the MIP-PEC sensor can specifically detect the target. Thus, The work has a linear range from 0.01 to 1000 ng mL?1 with a detection limit of 4.7 pg mL?1 for FB1. Furthermore, the fabricated MIP-PEC sensor will confirm the actual application.
关键词： Photoelectrochemical sensor,Molecularly imprinted polymer,fumonisin B1,GO-CdS heterojunction
更新于2025-09-09 09:28:46
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Hierarchical zinc oxide nanorings with superior sensing properties
摘要： Morphology controlled hydrothermal synthesis techniques have been realised to produce hierarchical zinc oxide (ZnO) nanostructures with high surface-to-volume ratios and more exposed polar facets. Hierarchical ZnO nanorings (HNRs) were produced by growing secondary nanowires (NWs) on initial mono-morphological nanodisks (NDs). In the two steps of growth process, zinc sulphate and zinc nitrate were used as the source of zinc ions, respectively. In comparison with NWs and NDs, the grown HNRs showed more enhanced performance as gas sensors. HNRs were substantially sensitive and faster in responding to acetone. This high performance in gas sensing is due to the increased surface-to-volume ratio and small size of the NW building blocks as well as the increased proportion of (0 0 0 1) polar facets. This work represents a guide to structure induced improvement of sensing properties by controlling the morphology of nanostructures.
关键词： Nanostructures,Sensor,Zinc oxide,Nanowires,Hydrothermal synthesis
更新于2025-09-09 09:28:46
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Tunable triple-band graphene refractive index sensor with good angle-polarization tolerance
摘要： In this article, we design a triple-band graphene refractive index sensor with good angle-polarization tolerance, which is composed of graphene elliptic-circular nanodisk resonators periodically arranged on a dielectric substrate. The numerical results indicate that the resonant wavelengths of three modes of graphene resonators show consecutively linear tunability when the refractive index of the surrounding medium varies, and the sensitivity is up to 11.56 μm/RIU. The sensing range can be further adjusted by controlling the doping level of graphene. In addition, the proposed graphene sensor maintains polarization insensitivity over a wide angular range (0-60°), showing very good angle-polarization tolerance. This work enables us to achieve a tunable triple-band sensor and provides potential value for label-free biomedical sensing.
关键词： graphene,surface plasmon resonance,refractive index sensor,metamaterials
更新于2025-09-09 09:28:46
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Hybrid structure Mach–Zehnder interferometer based on silica and fluorinated polyimide microfibers for temperature or salinity sensing in seawater
摘要： An all-fiber hybrid structure Mach-Zehnder interferometer (MZI) based on silica fiber and fluorinated polyimide microfiber (FPMF) for temperature or salinity sensing in seawater is proposed. Theoretically, transmission characteristic and sensing sensitivity of the MZI are derived for this kind of hybrid structure, and typical interference spectrum is observed around optical communication band. Experimentally, by tracking the shift of interference peaks, temperature and salinity sensing are demonstrated with sensitivities of about 140pm/°C and 64pm/‰, respectively, which are much higher than those of existing fiber Bragg gratings and microfiber knot resonators. In addition, dependences of sensitivity on sensor parameters, such as fiber diameter and fiber lengths, are also investigated. Unlike the other microfiber based sensors that higher sensitivity usually comes from the thinner fiber, sensors demonstrated here show that sensitivities can be tuned by changing the path-length difference of the two arms, which greatly decreases the difficulty in component fabrications. Temperature or salinity sensor demonstrated here show advantages of simple fabrication, low cost, tunable sensitivity and dynamic range, relatively high sensitivity and compatible with standard fiber optics system, which may have potential applications in developing low cost and compact size optical sensors in ocean or other liquid surroundings.
关键词： salinity,temperature,hybrid structure,microfiber,Optical fiber sensor,Mach-Zehnder interferometer
更新于2025-09-09 09:28:46
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Solution-synthesized chiral piezoelectric selenium nanowires for wearable self-powered human-integrated monitoring
摘要： Smart sensing devices with high stretchability and self-powered characteristics are essential in future generation wearable human-integrated applications. Here we report for the first time scalable synthesis and integration of selenium (Se) nanowires into wearable piezoelectric devices, and explore the feasibility of such devices for self-powered sensing applications, e.g., physiological monitoring. The ultrathin device can be conformably worn onto the human body, effectively converting the imperceptible time-variant mechanical vibration from the human body into distinguishable electrical signals, e.g., gesture, vocal movement, and radial artery pulse, through straining the piezoelectric Se nanowires. Our results suggest the potential of solution-synthesized Se nanowire a new class of piezoelectric nanomaterial for self-powered biomedical devices and opens doors to new technologies in energy, electronics, and sensor applications.
关键词： Self-powered sensor,Selenium nanowires,Wearable electronics,Human physiological monitoring,Piezoelectric device
更新于2025-09-09 09:28:46
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A multifunctional sensor for selective and sensitive detection of vitamin B12 and tartrazine by F?rster resonance energy transfer
摘要： We used thiamine nitrate (TN) as single material to fabricate nitrogen and sulfur co-doped carbon quantum dots (N,S-CQDs) with a quantum yield of 10.4% through one-pot hydrothermal method, and its properties were characterized by TEM, XPS, FTIR, fluorescence (FL) and UV-vis spectrophotometer, respectively. The fluorescence of N,S-CQDs was effectively quenched in the presence of vitamin B12 (VB12)/tartrazine due to F?rster resonance energy transfer (FRET). Moreover, the rate (KT) and efficiency (E%) of energy transfer from N,S-CQDs (as a donor) to VB12/tartrazine (as an acceptor) enhanced with increasing the concentrations of acceptor, and the KT and E% were also varied with the change of excitation wavelengths (from 338 to 408 nm). Based on this principle, a multifunctional fluorescence probe was designed for selective and sensitive detection of VB12/tartrazine with a detection limit (3σ/slope) of 15.6/18.0 nmol/L. Meanwhile, the proposed method was successfully employed to detect VB12/tartrazine in milk and several beverages with a recovery range of 97.5-104.2% /91.0-110.6%.
关键词： Tartrazine,Multifunctional sensor,FRET,N,S-CQDs,Vitamin B12
更新于2025-09-09 09:28:46
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Determination of particle distributions from sedimentation measurements using a piezoelectric tuning fork sensor
摘要： In this work, a resonant quartz tuning fork sensor based measurement system is employed to study the settlement of small particles in suspension as they pass the sensing element. The resonator features varying sensitivity to fluid parameter changes along its length, with the tip being most sensitive. The spatial averaging effect is analyzed theoretically and measured by passing the sensor through a fluid–fluid interface. Subsequently, the averaging function is used for particle distribution determination from recorded resonance frequency and quality factor measurements. The associated inverse problem is of Fredholm type and is solved numerically. Based on the system specifications, detection limits, required temperature stability and expected precision are assessed and particle distribution functions of defined test dispersions are measured.
关键词： Resonant sensor,Inverse problem,Suspension,Resonance estimation,Gout
更新于2025-09-09 09:28:46