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Src Kinase Controls Signaling Pathways In Sensory Neuron Triggered By Low-Power Infrared Radiation
摘要： Low-power (non-thermal) infrared (IR) radiation with the wavelength of 10.6 μm activates the Na,K-ATPase transducer function in sensory neurons, which is manifested in decrease of NaV1.8 channel voltage sensitivity at the cellular membrane level and in inhibition of growth of chick embryo dorsal root ganglia neurites at the tissue level. It is shown that the effect of low-power IR radiation is totally blocked by a specific Src kinase inhibitor PP2. Upon irradiation on the background of PP2, the effective charge of NaV1.8 channel activation gating system does not differ from its control value in patch-clamp experiments, and the area index of sensory ganglia neurites growth remains unchanged as compared to the control in organotypic tissue culture. The data obtained demonstrate that Src kinase is involved in intracellular signaling pathways triggered by CO2 laser low-power IR radiation by the transducer-activated mechanism. This is the first indication that in primary sensory neuron the signals of low-power IR radiation are sensed, amplified and transduced by the Na,K-ATPase/Src complex and not by G proteins.
关键词： Src kinase,infrared radiation,Na,K-ATPase,organotypic nerve tissue culture,NaV1.8 channels,sensory neuron,patch-clamp method
更新于2025-11-21 11:24:58
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Stomatal response to blue light in crassulacean acid metabolism plants <i>Kalanchoe pinnata</i> and <i>Kalanchoe daigremontiana</i>
摘要： Blue light (BL) is a fundamental cue for stomatal opening in both C3 and C4 plants. However, it is unknown whether crassulacean acid metabolism (CAM) plants open their stomata in response to BL. We investigated stomatal BL responses in the obligate CAM plants Kalanchoe pinnata and Kalanchoe daigremontiana that characteristically open their stomata at night and close them for part of the day, as contrasted with C3 and C4 plants. Stomata opened in response to weak BL superimposed on background red light in both intact leaves and detached epidermal peels of K. pinnata and K. daigremontiana. BL-dependent stomatal opening was completely inhibited by tautomycin and vanadate, which repress type 1 protein phosphatase and plasma membrane H+-ATPase, respectively. The plasma membrane H+-ATPase activator fusicoccin (FC) induced stomatal opening in the dark. Both BL and FC induced phosphorylation of the guard cell plasma membrane H+-ATPase in K. pinnata. These results indicate that BL-dependent stomatal opening occurs in the obligate CAM plants K. pinnata and K. daigremontiana independently of photosynthetic CO2 assimilation mode.
关键词： phototropins,CAM plants,plasma membrane H+-ATPase,stomatal opening,blue light,signal transduction
更新于2025-09-23 15:23:52
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Complexation of the Mycotoxin Cyclopiazonic Acid with Lanthanides Yields Luminescent Products
摘要： Cyclopiazonic acid (CPA) is a neurotoxin that acts through inhibition of the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA). CPA blocks the calcium access channel of the enzyme. The inhibition may involve the binding of CPA with a divalent cation such as Mg2+. The potential for CPA to act as a chelator also has implications for methods to detect this toxin. Certain of the lanthanide metals undergo a dramatic increase in luminescence upon coordination with small molecules that can transfer excitation energy to the metal. This report is the first to describe the coordination of CPA with lanthanide metals, resulting in a substantial enhancement of their luminescence. The luminescence expressed was dependent upon the type of lanthanide, its concentration, and the environment (solvent, water content, pH). Based upon the phenomenon, a competitive assay was also developed wherein terbium (Tb3+) and a series of metal cations competed for binding with CPA. With increasing cation concentration, the luminescence of the CPA/Tb3+ complex was inhibited. The chlorides of ten metals were tested. Inhibition was best with Cu2+, followed by Co2+, Al3+, Fe3+, Mn2+, Au3+, Mg2+, and Ca2+. Two cations in oxidation state one (Na+, K+) did not inhibit the interaction significantly. The interaction of CPA with lanthanides provides a novel recognition assay for this toxin. It also provides a novel way to probe the binding of CPA to metals, giving insights into CPA's mechanism of action.
关键词： mechanism of action,lanthanides,calcium-ATPase,luminescence,mycotoxin,cyclopiazonic acid
更新于2025-09-23 15:22:29
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Multifunctional single-layered graphene quantum dots used for diagnosis of mitochondrial malfunction-related diseases
摘要： Mitochondria are critical organelles in eukaryotes that efficiently generate ATP for various biological activities and any defect in the process of ATP synthesis may lead to mitochondrial dysfunction and directly link to a variety of medical disorders. Monitoring the ATP variations in cells is key for innovative early diagnosis of mitochondrial diseases. Herein, multifunctional single-layered graphene quantum dots (s-GQDs) with bright green emission were constructed, which exhibit strong binding a?nity for ATP and good mitochondria targeting ability. Using the proposed s-GQDs we successfully achieved to discriminate the primary smooth muscle cells isolated from the transgenic mouse (heterozygote Sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) 2 C674S knock-in mouse) with mitochondrial disorders or their littermate controls, indicating s-GQDs as promising probes for the study of cell metabolism and mitochondrial malfunction-related diseases, and targeting endoplasmic reticulum stress is an effective way to modulate metabolic pathways relevant to SERCA 2 inactivity mitochondrial dysfunction.
关键词： graphene quantum dots,ATP,mitochondrial disorders,endoplasmic reticulum stress,sarco/endoplasmic reticulum Ca2+ ATPase
更新于2025-09-12 10:27:22
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Silica Three-Dimensional Biosensors
摘要： Around the world, food-borne virus is one of the main pathogenic microorganisms in the aspect of causing people and animals with acute diarrhoea and poses a serious threat to human health. Therefore, the rapid detection of food-borne virus is very important to guarantee food safety and human health. Here, we reported that we developed a specific, convenient and fast detected method to detect rotavirus (RV) and hepatitis A virus (HAV) by using F0F1-ATPase molecular motor biosensor. Specific RNA probes were encompassed the conservative region of food-borne virus, and a molecular motor detect device was constructed by connecting probes to F0F1-ATPase molecular motor through biotin-streptavidin system. Extracted virus RNA was conjugated with the biosensor separately and meanwhile ATP was synthesized. By comparing their fluorescence intensity, virus RNA level was detected. Our results demonstrated that this biosensor’s sensitivity was the concentrations of 0.005 ng/mL and 0.01 ng/mL for RV and HAV respectively. Furthermore, this method possessed specificity for RV and HAV and none cross-reaction between them. What’s more, this method could be accomplished within 1h. We detected 15 samples by using this method and the results were consistent with RT-PCR results. Overall, this new-typed method based on F0F1-ATPase molecular motor biosensor for RV and HAV detection is sensitive and specific and can be used in the rapid detection of food-borne virus.
关键词： F0F1-ATPase,Detection,Molecular motor biosensor,Food-borne virus
更新于2025-09-04 15:30:14