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Antimicrobial-Peptide-Conjugated MoS2 Based Nanoplatform for Multimodal Synergistic Inactivation of Superbugs
摘要： Development of new antibacterial therapeutics material is becoming increasingly urgent due to the huge threat of superbugs, which are responsible for more than half million death each year in this world. Here, we report the development of novel nano-biomaterial based on melittin antimicrobial peptide (AMP) attached transition metal dichalcogenide MoS2 based theranostic nanoplatform. Reported nanoplatform has capability for targeted identification and synergistic inactivation of 100% multidrug-resistant superbugs by combined photo thermal therapy (PTT), photodynamic therapy (PDT) and AMP process. A novel approach for the design of melittin antimicrobial peptide attached MoS2 based nanoplatform is reported, which emits very bright and photo stable fluorescence. It also generates heat as well as reactive oxygen species (ROS) in the presence of 670 nm near infrared light, which allow it to be used as PTT & PDT agent. Due to the presence of AMP, multifunctional AMP exhibits significantly improved antibacterial activity for superbugs via multimodal synergistic killing mechanism. Reported data demonstrate that nanoplatforms are capable of identification of multidrug-resistant superbugs via luminescence imaging. Experimental results show that it is possible to kill only ~45% of superbugs via MoS2 nanopaltform based on PTT & PDT processes together. On the other hand, killing of less than 10% of superbugs is possible using melittin antimicrobial peptide alone. Whereas, 100% Methicillin-resistant Staphylococcus aureus (MRSA), drug resistance Escherichia coli (E. coli) and drug resistance Klebsiella pneumoniae (KPN) superbugs can be killed using antimicrobial peptide attached MoS2 QDs, via synergistic killing mechanism. Mechanisms for possible synergistic killing of multidrug-resistant superbugs have been discussed.
关键词： theranostic transition metal dichalcogenide,photodynamic therapy,multimodal therapy for multidrug-resistant superbugs,Melittin antimicrobial peptide attached MoS2 based nanoplatform,photo thermal therapy
更新于2025-09-23 15:23:52
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A molecular hybrid producing simultaneously singlet oxygen and nitric oxide by single photon excitation with green light
摘要： Combination of photosensitizers (PS) for photodynamic therapy with NO photodonors (NOPD) is opening intriguing horizons towards new and still underexplored multimodal anticancer and antibacterial treatments not based on “conventional” drugs and entirely controlled by light stimuli. In this contribution, we report an intriguing molecular hybrid based on a BODIPY light-harvesting antenna that acts simultaneously as PS and NOPD upon single photon excitation with the highly biocompatible green light. The presented hybrid offers a combination of superior advantages with respect to the other rare cases reported to date, meeting most of the key criteria for both PSs and NOPDs in the same molecular entity such as: (i) capability to generate 1O2 and NO with single photon excitation of biocompatible visible light, (ii) excellent 1O2 quantum yield and NO quantum efficiency, (iii) photogeneration of NO independent from the presence of oxygen, (iv) large light harvesting properties in the green region. Furthermore, this compound together with its stable photoproduct, is well tolerated by both normal and cancer cells in the dark and exhibits bimodal photomortality of cancer cells under green light excitation due to the combined action of the cytotoxic 1O2 and NO.
关键词： Green light,Nitric oxide,Multimodal therapy,Singlet oxygen,BODIPY,Photodynamic therapy
更新于2025-09-23 15:22:29
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Combination of light-driven co-delivery of chemodrugs and plasmonic-induced heat for cancer therapeutics using hybrid protein nanocapsules
摘要： Background: Improving the water solubility of hydrophobic drugs, increasing their accumulation in tumor tissue and allowing their simultaneous action by different pathways are essential issues for a successful chemotherapeutic activity in cancer treatment. Considering potential clinical application in the future, it will be promising to achieve such purposes by developing new biocompatible hybrid nanocarriers with multimodal therapeutic activity. Results: We designed and characterised a hybrid nanocarrier based on human serum albumin/chitosan nanoparticles (HSA/chitosan NPs) able to encapsulate free docetaxel (DTX) and doxorubicin?modified gold nanorods (DOXO?GNRs) to simultaneously exploit the complementary chemotherapeutic activities of both antineoplasic compounds together with the plasmonic optical properties of the embedded GNRs for plasmonic?based photothermal therapy (PPTT). DOXO was assembled onto GNR surfaces following a layer?by?layer (LbL) coating strategy, which allowed to partially control its release quasi?independently release regarding DTX under the use of near infrared (NIR)?light laser stimulation of GNRs. In vitro cytotoxicity experiments using triple negative breast MDA?MB?231 cancer cells showed that the developed dual drug encapsulation approach produces a strong synergistic toxic effect to tumoral cells compared to the administration of the combined free drugs; additionally, PPTT enhances the cytostatic efficacy allowing cell toxicities close to 90% after a single low irradiation dose and keeping apoptosis as the main cell death mechanism. Conclusions: This work demonstrates that by means of a rational design, a single hybrid nanoconstruct can simultaneously supply complementary therapeutic strategies to treat tumors and, in particular, metastatic breast cancers with good results making use of its stimuli?responsiveness as well as its inherent physico?chemical properties.
关键词： Multimodal therapy,Gold nanorods,Photo?therapy,Stimuli?responsiveness,Human serum albumin nanoparticles
更新于2025-09-19 17:13:59
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Surgical excision combined with fully ablative carbon dioxide laser therapy and triamcinolone injections as a potential treatment for keloids in children
摘要： Background/objective: Keloids represent a functional and esthetic burden and can be particularly challenging to treat. Various topical, injectable, and ablative therapies exist but are associated with significant recurrence if used alone. We wanted to evaluate the long-term efficacy of multimodal therapy in pediatric patients treated for keloids at the Sainte-Justine University Hospital Center. Methods: We conducted a retrospective case study of 21 children with 21 keloids treated between April 2006 and April 2016. The following combination of treatments was studied: surgical excision, carbon dioxide (CO2) laser therapy on the surgical site, and triamcinolone acetonide (TAC) intralesional injections during surgery and follow-up. Depending on the site treated, pressure garments and/or topical silicone were also used. Results: Of the 21 patients initially treated, four were lost to follow-up. Of the 17 patients who had adequate follow-up (mean duration 18 months, with periodic reassessments and additional TAC injections if needed), 6 (35%) had some recurrence, with remission in three of them following subsequent treatment and three lost to follow-up. In summary, 14 (82.4%) of the patients with adequate follow-up had complete clearance of the keloid. However, this must be interpreted in light of the fact there was a significant loss of follow-up (33.3%) from the initial cohort. Conclusion: Our results are in agreement with other recent studies that demonstrate the need for multimodal therapy with combined methods in order to achieve long-term remission. A randomized controlled trial would be necessary to assess the real benefit of this treatment combination in children.
关键词： scar,multimodal therapy,keloid,CO2 laser,corticosteroid injection,surgery
更新于2025-09-11 14:15:04