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Digital Single Virus Immunoassay for Ultrasensitive Multiplex Avian Influenza Virus Detection Based on Fluorescent Magnetic Multifunctional Nanospheres
摘要： Fluorescence method has made great progress in the construction of sensitive sensors, but the background fluorescence of matrix and photobleaching limit its broad application in clinical diagnosis. Here, we propose a digital single virus immunoassay for multiplex virus detection by using fluorescent magnetic multifunctional nanospheres as both capture carriers and signal labels. The superparamagnetism and strong magnetic response ability of nanospheres can realize efficient capture and separation of targets without sample pretreatment. Due to its distinguishable fluorescence imaging and photostability, the nanospheres enable single-particle counting for ultrasensitive multiplexed detection. Furthermore, the integration of digital analysis provided a reliable quantitative strategy for rare targets detection. Based on multifunctional nanospheres and digital analysis, a digital single virus immunoassay was proposed for simultaneous detection of H9N2, H1N1 and H7N9 avian influenza virus (AIV) without complex signal amplification, whose detection limits were 0.02 pg/mL. Owing to its good specificity and anti-interference ability, the method showed great potential in single biomolecules, multiplexed detection and early diagnosis of diseases.
关键词： Multifunctional nanospheres,Digital analysis,Avian influenza virus,Single virus immunoassay,Ultrasensitive multiplexed detection
更新于2025-09-19 17:15:36
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High-quality quantum dots for multiplexed bioimaging: A critical review
摘要： Bioimaging carried out using two or more fluorophores possessing different emission wavelength can be termed as a multicolor/multiplexed bioimaging technique. Traditionally, images are captured sequentially using multiple fluorophores having specific excitation and emission. For this purpose, multifunctional nanoprobes, such as organic fluorophores, metallic nanoparticles, semiconductor quantum dots, and carbon dots (CDs) are used. Among these fluorophores, quantum dots (QDs) have emerged as an ideal probe for multiplexed bioimaging due to their unique property of size tunable emission. However, the usage of quantum dots in bioimaging is limited due to their toxicity. Furthermore, the reproducibility of optical properties is cynical. These desirable properties, along with enhancement in quantum efficiency, photostability, fluorescence lifetime, etc. can be achieved using precision control over synthesis parameters. This review summarizes the desirable properties and synthesis methods of such superior QDs followed by their application in multiplexed imaging.
关键词： Multiplexed Imaging,Quantum dots,Microreactor,Bioimaging,Organic Dyes
更新于2025-09-19 17:13:59
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Investigation of Inverse Solutions for Tilting Orthogonal Double Prisms in Laser Pointing with Submicroradian Precision
摘要： This paper reports the design, fabrication, and characterization of arrays of miniaturized, internally fed, polymer electrospray emitters fabricated with stereolithography. The freeform additive manufacturing process used to make the devices has associated two orders of magnitude reduction in the fabrication cost per device and fabrication time (from thousands of dollars to tens of dollars, and from months to hours, respectively) and a two orders of magnitude reduction in the cost of the manufacturing infrastructure (from millions of dollars to tens of thousands of dollars) compared with a silicon MEMS multiplexed electrospray source. The 3-D printed devices include features not easily attainable with other microfabrication methods, e.g., tapered channels and threaded holes. Through the optimization of the fabrication process 10-mm tall, isolated, straight, solid columns with diameter as small as 300 μm, and 12-mm long, straight tubes with inner diameter as small as 400 μm and wall thickness as small as 150 μm were demonstrated. Arrays with as many as 236 internally fed electrospray emitters (236 emitters in 1 cm2) were made, i.e., a twofold increase in emitter density and a sixfold increase in array size compared with the best reported values from multiplexed, internally fed, electrospray sources made of polymer. The characterization of devices with a different array size suggests a uniform emitter operation.
关键词： electrospray,Additive manufacturing of MEMS,stereolithography,multiplexed liquid ionizers
更新于2025-09-19 17:13:59
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[IEEE 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Munich, Germany (2019.6.23-2019.6.27)] 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Frequency-Multiplexed Singly-Resonant Photon Pairs from a Quadratic Nonlinear Waveguide Resonator
摘要： Frequency multiplexed photon pair generation has been actively studied for efficient and large-scale photonic quantum information processing [1-3]. In these demonstrations, photon pair generators were realized by using an optical parametric oscillator (OPO) far below threshold; a nonlinear optical medium was inside or integrated with an optical cavity, and a sufficiently weak pump light for the photon pair generation was used. However, to the best of our knowledge, all of the previous demonstrations for frequency multiplexed photon pair generation have used a doubly-resonant OPO which confines both the signal and the idler photons, or a triply-resonant OPO which additionally confines the pump light. In this study, we demonstrate a frequency multiplexed photon pair generation based on a singly-resonant (SR) OPO configuration which confines only one half of the photon pair. We used a periodically-poled lithium niobate (PPLN) waveguide as a monolithically-integrated quadratic nonlinear waveguide resonator; dielectric multilayers are directly formed at the end faces of the PPLN waveguide such that only the signal photon around 1600 nm of the photon pair is confined in the resonator and the idler photon around 1522 nm and the pump light at 780 nm are not confined. In the SR photon pair, in spite of no cavity configuration for the idler photons, not only the signal photons but also the idler photons have comb-like spectra as if both of them are confined in the cavity. In addition, there is not missing teeth region observed in multiple resonant OPO configuration known as the cluster effect [4,5]. As a result, a wide-band frequency multiplexed photon pair generation can be possible. The photon pair generator will be useful for an efficient photonic quantum information processing and a frequency-domain photon manipulation with the use of frequency-domain optical elements [6-9].
关键词： singly-resonant OPO,PPLN waveguide,photon pair generation,quantum information processing,frequency multiplexed
更新于2025-09-16 10:30:52
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[IEEE 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Munich, Germany (2019.6.23-2019.6.27)] 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - An Entanglement-Based Wavelength Multiplexed Quantum Communication Network
摘要： Quantum networks scale the advantages of quantum communication protocols to more than just two distant users. Here we present a fully connected quantum network architecture in which a single entangled photon source distributes quantum states to a multitude of users [1]. Our network architecture thus minimizes the resources required of each user without sacrificing security or functionality. As no adaptations of the source are required to add users, the network can readily be scaled to a large number of clients, whereby no trust in the provider of the quantum source is required. Unlike previous attempts at multi-user networks, which have been based on active components, and thus limited to some duty cycle, our implementation is fully passive and thus provides the potential for unprecedented quantum communication speeds. We experimentally demonstrate the feasibility of our approach using a single source of bi-partite polarization entanglement which is multiplexed into 12 wavelength channels to distribute 6 states between 4 users in a fully connected graph using only 1 fiber and polarization analysis module per user.
关键词： wavelength multiplexed,entanglement,quantum network,quantum communication
更新于2025-09-16 10:30:52
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[IEEE 2019 17th International Conference on ICT and Knowledge Engineering (ICT&KE) - Bangkok, Thailand (2019.11.20-2019.11.22)] 2019 17th International Conference on ICT and Knowledge Engineering (ICT&KE) - Provision and Visualization of Solar Radiation Data for Energy Management System
摘要： This paper reports the design, fabrication, and characterization of arrays of miniaturized, internally fed, polymer electrospray emitters fabricated with stereolithography. The freeform additive manufacturing process used to make the devices has associated two orders of magnitude reduction in the fabrication cost per device and fabrication time (from thousands of dollars to tens of dollars, and from months to hours, respectively) and a two orders of magnitude reduction in the cost of the manufacturing infrastructure (from millions of dollars to tens of thousands of dollars) compared with a silicon MEMS multiplexed electrospray source. The 3-D printed devices include features not easily attainable with other microfabrication methods, e.g., tapered channels and threaded holes. Through the optimization of the fabrication process 10-mm tall, isolated, straight, solid columns with diameter as small as 300 μm, and 12-mm long, straight tubes with inner diameter as small as 400 μm and wall thickness as small as 150 μm were demonstrated. Arrays with as many as 236 internally fed electrospray emitters (236 emitters in 1 cm2) were made, i.e., a twofold increase in emitter density and a sixfold increase in array size compared with the best reported values from multiplexed, internally fed, electrospray sources made of polymer. The characterization of devices with a different array size suggests a uniform emitter operation.
关键词： electrospray,Additive manufacturing of MEMS,stereolithography,multiplexed liquid ionizers
更新于2025-09-16 10:30:52
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Fluorometric lateral flow immunoassay for simultaneous determination of three mycotoxins (aflatoxin B1, zearalenone and deoxynivalenol) using quantum dot microbeads
摘要： A fluorometric lateral flow immunoassay (LFA) is described for the simultaneous determination of the mycotoxins aflatoxin B1 (AFB1), zearalenone (ZEN) and deoxynivalenol (DON). The method is based on the use of CdSe/SiO2 quantum dot microbeads (QBs) with a mean diameter of 106 nm. These have strong red luminescence (with excitation/emission peaks at 365/622 nm) which results in enhanced sensitivity. The QBs binding with monoclonal antibodies (mAbs) as the signal probes can react specifically with AFB1, ZEN and DON, respectively. There is an inverse correlation between the fluorescence signal intensity of test line and the analyte content, which can realize the quantitative analysis of analytes within 15 min. The limits of detection in solution are 10, 80 and 500 pg mL?1 for AFB1, ZEN and DON, respectively. Besides, the average recoveries from spiked feed range from 85.5 to 119.0%, and the relative standard deviations are less than 16.4% for both intra- and inter-day assays. The method was used to analyze naturally contaminated feedstuff, and this resulted in a good agreement with data obtained by LC-MS/MS.
关键词： Rapid detection,LC-MS/MS,Food safety,Multiplexed,Immunochromatographic strip,Quantitative analysis,Cereal samples,Antibody,Feedstuff
更新于2025-09-12 10:27:22
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Frequency-Multiplexed Photon Pairs Over 1000 Modes from a Quadratic Nonlinear Optical Waveguide Resonator with a Singly Resonant Configuration
摘要： We demonstrate a frequency multiplexed photon pair generation based on a quadratic nonlinear optical waveguide inside a cavity which confines only signal photons without confining idler photons and the pump light. We monolithically constructed the photon pair generator by a periodically poled lithium niobate (PPLN) waveguide with a high reflective coating for the signal photons around 1600 nm and with antireflective coatings for the idler photons around 1520 nm and the pump light at 780 nm at the end faces of the PPLN waveguide. We observed a comblike photon pair generation with a mode spacing of the free spectral range of the cavity. Unlike the conventional multiple resonant photon pair generation experiments, the photon pair generation was incessant within a range of 80 nm without missing teeth due to a mismatch of the energy conservation and the cavity resonance condition of the photons, resulting in over 1000-mode frequency multiplexed photon pairs in this range.
关键词： frequency multiplexed photon pair generation,photon pair generator,PPLN waveguide,quadratic nonlinear optical waveguide,singly resonant configuration
更新于2025-09-12 10:27:22
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[IEEE 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Munich, Germany (2019.6.23-2019.6.27)] 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - High Efficiency Quantum Memory in Multiplexed Large-OD Cold Atomic Ensemble
摘要： Quantum memories enabling the storage of an input photonic qubit and its later retrieval with a ?delity beating any classical device constitute essential components in quantum communication networks and optical quantum information processing [1]. Our main goal is to develop a new generation of memories which have a near-unity ef?ciency for storage-and-retrieval operations, as well as high multiplexing capabilities. In the recent years, we demonstrated for instance the implementation for quantum bits encoded in the orbital angular momentum degree of freedom, which provides an essential capability for future networks with multimode capability [2]. We also realized multiple-degree-of-freedom memory, which can ?nd applications in classical data processing but also in quantum network scenarios where states structured in phase and polarization have been shown to provide promising attributes [3]. Recently, we realized a multiplexed quantum memory for polarization encoded qubits with high storage-and-retrieval ef?ciency [4]. We report on a quantum memory for polarization qubits that combines an average conditional ?delity above 99% and ef?ciency around 68%, thereby demonstrating a reversible qubit mapping where more information is retrieved than lost. The qubits are encoded with weak coherent states at the single-photon level and the memory is based on electromagnetically-induced transparency in an elongated laser-cooled ensemble of cesium atoms, spatially multiplexed for dual-rail storage. The reported ef?ciency approaches the maximal performance achievable on the D2 line transition used here, as shown by a comprehensive model that includes all the involved atomic transitions. In the alkali-metal atoms, hyper?ne interaction in the excited state indeed introduces several levels which can have a strong effect on the medium susceptibility via off resonant excitation, thereby decreasing the ef?ciency when the optical depth (OD) is large. As shown by this model, switching to the D1 line can increase the ef?ciency above 90%. Preliminary results with optical depth around 400 led us to a storage-and-retrieval ef?ciency of 85% at the single-photon level.
关键词： Large-OD,Quantum memory,Polarization qubits,Multiplexed,Cesium atoms,Cold atomic ensemble,Electromagnetically-induced transparency
更新于2025-09-11 14:15:04
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Towards rational design and optimization of near-field enhancement and spectral tunability of hybrid core-shell plasmonic nanoprobes
摘要： In biology, sensing is a major driver of discovery. A principal challenge is to create a palette of probes that offer near single-molecule sensitivity and simultaneously enable multiplexed sensing and imaging in the “tissue-transparent” near-infrared region. Surface-enhanced Raman scattering and metal-enhanced fluorescence have shown substantial promise in addressing this need. Here, we theorize a rational design and optimization strategy to generate nanostructured probes that combine distinct plasmonic materials sandwiching a dielectric layer in a multilayer core shell configuration. The lower energy resonance peak in this multi-resonant construct is found to be highly tunable from visible to the near-IR region. Such a configuration also allows substantially higher near-field enhancement, compared to a classical core-shell nanoparticle that possesses a single metallic shell, by exploiting the differential coupling between the two core-shell interfaces. Combining such structures in a dimer configuration, which remains largely unexplored at this time, offers significant opportunities not only for near-field enhancement but also for multiplexed sensing via the (otherwise unavailable) higher order resonance modes. Together, these theoretical calculations open the door for employing such hybrid multi-layered structures, which combine facile spectral tunability with ultrahigh sensitivity, for biomolecular sensing.
关键词： plasmonic nanoprobes,near-field enhancement,hybrid core-shell,biomolecular sensing,spectral tunability,multiplexed sensing
更新于2025-09-11 14:15:04