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Silver nanosol SERS quantitative analysis of ultratrace biotin coupled N-doped carbon dots catalytic amplification with affinity reaction
摘要： Highly catalytic and stable N-doped carbon dots (N-CDs) were prepared rapidly by microwave procedure using glucose as precursor and ammonium sulfite as N-dopant. The reduction of AgNO3 by trisodium citrate (TCA) was slow to form nanosilver (AgNP), and the N-CDs exhibited strong catalysis of the AgNP reaction. The formed AgNPs were used as indicator in the presence of Vitoria blue B (VBB) molecule probe with a SERS peak at 1615 cm?1. With the increase of nanocatalyst N-CDs concentration, the AgNP reaction speed up, and the SERS peak of VBB enhanced linearly due to formation of more AgNPs as substrate. In the presence of avidin (Ad), the SERS peak weakened. Upon addition of biotin, the SERS peak enhanced due to turn on the indicator nanoreaction. The enhanced SERS signal had a good linear relationship with the biotin concentration in range of 0.0006–0.021 ng/mL, with a detection limit of 0.3 pg/mL.
关键词： Nanocatalysis,N-doped carbon dots,SERS,Affinity reaction,Biotin
更新于2025-09-23 15:19:57
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A Highly Sensitive SERS and RRS Coupled Di-Mode Method for CO Detection Using Nanogolds as Catalysts and Bifunctional Probes
摘要： Carbon monoxide (CO) is a commonly poisonous gas. It is important to detect CO in daily life. Herein, a new and sensitive surface enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) coupled di‐mode method was developed for CO, based on gold nano‐enzyme catalysis and gold nanoprobes. CO can react with HAuCl4 to generate gold nanoparticles (AuNPs) in pH 5.2 HAc‐NaAc buffer. The generated AuNPs exhibited SERS activity at 1620 cm‐1 in the presence of Vitoria blue B (VBB) molecular probes, and an RRS peak at 290 nm. Based on the AuNP bifunctional probes, the increased SERS and RRS intensities respond linearly with the concentration of CO in the range of 100–1500 ng/mL and 30–5230 ng/mL, respectively. To improve the sensitivity, the produced AuNPs were used as nano‐enzyme catalysts for the new indicator reaction of HAuCl4‐ethanol (En) to amplify the signal. The sensitive SERS method was coupled with the accurate RRS method to develop a sensitive and accurate SERS/RRS di‐mode method for determination of 3.0–413 ng/mL CO, based on the AuNP‐HAuCl4‐En nanocatalytic reaction and its product of AuNPs as SERS and RRS bifunctional probes.
关键词： coupled di‐mode,SERS,nanocatalysis,CO,RRS
更新于2025-09-19 17:13:59