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Electrochemiluminescent immunoassay for neuron specific enolase by using amino-modified reduced graphene oxide loaded with N-doped carbon quantum dots
摘要： An ultrasensitive electrochemiluminescence based sandwich immunoassay is presented for determination of neuron specific enolase. The method uses silver-cysteine nanowires as the capture probe and a composite made of amino-modified reduced graphene oxide and nitrogen-doped carbon quantum dots as the signal probe. It was synthesized by covalent coupling of amino-modified reduced graphene oxide to the carboxy groups of nitrogen-doped carbon quantum dots. The nanowires possess a large specific surface and abundant functional groups which facilitate immobilizing the primary antibody (Ab1). The amino-modified reduced graphene oxide is employed as a carrier for loading a large number of the quantum dots and secondary antibody (Ab2). This increases the electrochemiluminescence intensity of quantum dots. Response to neuron specific enolase is linear in the 0.55 fg·mL?1 to 5.5 ng·mL?1 concentration range. It has a detection limit of 0.18 fg·mL?1 (at S/N = 3). The relative standard deviation (for n = 6) is less than 2.9%. The assay is highly sensitive, reproducible, selective and stable.
关键词： Silver-cysteine nanowires,Sensor,Neuron specific enolase,Carbon quantum dots,Graphene oxide
更新于2025-09-11 14:15:04
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Enzyme-free multicolor biosensor based on Cu2+-modified carbon nitride nanosheets and gold nanobipyramids for sensitive detection of neuron specific enolase
摘要： In this work, we have synthesized Cu2+-modified carbon nitride nanosheets (Cu2+-C3N4) as peroxidase mimic catalytic substance, which is more active than the horseradish peroxidase enzyme in extreme environments. Gold nanobipyramid (Au NBP) is a good chromogenic substrate for multicolor display because the longitudinal plasmon bands of AuNRs can be easily tuned by adjusting their aspect ratios and used as an excellent indicator for colorimetric detection of immunoassays. The generation of TMB2+ from sandwich complex (peroxidase-like catalysis) efficiently etches Au NPBs to produce multicolor variations from brown to olive, green, blue, purple, purple, red, pink, and colorless in presence of varied concentrations of neuron specific enolase (NSE). The experimental results show that the colorimetric detection ranging from 312.5 to 20,000 pM with a detection limit of 92.8 pM, which is higher than other multicolor based sensors. The Cu2+-C3N4 nanosheets and Au NBPs based colorimetric visual (naked eye) semi-quantitative method as a potential platform towards the detection of important biomolecules in clinical and therapeutic applications. The proposed method is simple, low-cost and enzyme-free to detect NSE for the first time.
关键词： Gold nanobipyramids,Cu2+-C3N4 nanosheets,Mimic enzyme activity,Neuron specific enolase,Biosensor
更新于2025-09-04 15:30:14
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Fabrication of Immunosensor Based on Au-silica Nanocomposite for Neuron-specific Enolase Detection
摘要： Neuron-specific enolase (NSE), a type of neuroendocrine molecule, is a putative serum marker for small-cell lung carcinoma. This work addressed the fabrication of a signal-enhanced, label-free, electrochemical immunosensor based on Au nanoparticle/mesoporous silica nanoparticles (Au-MSNs) for NSE determination. Because of the large number of active sites provided by the Au-SiO2 sensor, the modified immunosensor has a significantly enhanced sensitivity. Furthermore, during quantitative NSE detection, the sensor has a desirable linear relationship with the concentration (0.1-2000 ng/mL) and a detection limit of 0.05 ng/mL.
关键词： Neuron-specific enolase (NSE),Label-free,Electrochemical immunosensors,Silica immunosensor,Signal-enhanced
更新于2025-09-04 15:30:14