- 标题
- 摘要
- 关键词
- 实验方案
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THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis
摘要： To elucidate dynamic developmental processes in plants, live tissues and organs must be visualised frequently and for extended periods. The development of roots is studied at a cellular resolution not only to comprehend the basic processes fundamental to maintenance and pattern formation but also study stress tolerance adaptation in plants. Despite technological advancements, maintaining continuous access to samples and simultaneously preserving their morphological structures and physiological conditions without causing damage presents hindrances in the measurement, visualisation and analyses of growing organs including plant roots. We propose a preliminary system which integrates the optical real-time visualisation through light microscopy with a liquid culture which enables us to image at the tissue and cellular level horizontally growing Brachypodium roots every few minutes and up to 24 h. We describe a simple setup which can be used to track the growth of the root as it grows including the root tip growth and osmotic stress dynamics. We demonstrate the system’s capability to scale down the PEG-mediated osmotic stress analysis and collected data on gene expression under osmotic stress.
关键词： root,real-time imaging,osmotic stress,Casparian bands,Brachypodium,PDMS,neutral red,PEG-6000
更新于2025-09-23 15:22:29
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Reduced graphene oxide supported MnO2 nanorods as recyclable and efficient adsorptive photocatalysts for pollutants removal
摘要： The emerging concept of two dimensional (2D) hybrid materials with large surface area and good interfacial contacts is highly desirable for diverse catalytic applications. Herein, we have designed and developed novel 1D-2D nanocomposite by loading manganese dioxide (MnO2) nanorods over reduced graphene oxide (RGO) nanosheets by facile hydrothermal synthesis method to exploit the large surface area, close contact of 1D-2D components with abundant reaction sites. The as prepared MnO2-RGO nanocomposite has been characterized in detail using state-of-art techniques and has been successfully utilized efficient adsorptive photocatalysts for the removal of a colored dye (neutral red) and a colorless pollutant (ciprofloxacin) from water. In addition, the stability and recyclability of this catalyst has also been demonstrated. This work is expected to pave way for the development of many new 1D-2D binary nanocomposite catalysts for environmental remediation applications.
关键词： Reduced graphene oxide,MnO2 nanorods,Photocatalysis,Pollutants removal,Ciprofloxacin,Neutral red
更新于2025-09-23 15:21:21