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Auto-cleaning paper-based electrochemiluminescence biosensor coupled with binary catalysis of cubic Cu2O-Au and polyethyleneimine for quantification of Ni2+ and Hg2+
摘要： Inspired by the pop-up greeting cards, a 3D collapsible auto-cleaning paper-based electrochemiluminescence (ECL) biosensor (CAPEB) with different functions of signal collection and residual multiple cleaning, is developed for sensitive detection of Ni2+ and Hg2+ by simply regulating its 3D configurations. The multiple fluidic paths and the hollow-channel structure were firstly integrated into the paper substrate, realizing simultaneously repetitive auto-cleaning of the two working electrodes. For achieving ultrasensitive Ni2+ and Hg2+ monitoring, binary catalysis consisting of the intermolecular co-reaction (H2O2 and N-(4-Aminobutyl)-N-ethylisoluminol (ABEI)) and intramolecular catalysis (polyethyleneimine (PEI)-ABEI) were introduced. Specifically, silver nanospheres with a large specific surface area and excellent conductivity were grown on the paper working electrode and served as the sensor substrate for fixing PEI-ABEI and Ni2+-specific DNAzyme. With the assistance of DNAzyme, Cu2O-Au and ferrocene (Fc) labeled strand S2 were immobilized on electrode surface through the hybridization reaction, and catalyzed H2O2 to generate reactive oxygen species, promoting the luminescence of ABEI. In the existence of Ni2+, DNAzyme was activated followed by cleavage of strand S2 to induce the release of Fc, which quenched the ECL signal of ABEI, eventually realizing the detection of Ni2+. Similarly, for sensitive quantification of Hg2+, full thymine (T) bases strand S3 was modified on surface of Cu2O-Au and anchored Hg2+ by T-Hg2+-T pairing interaction. The ECL intensity was decreased along with increasing of Hg2+ due to the quenching effect of Hg2+ on ECL emission of ABEI. Based on this ingenious system, the detection of Ni2+ and Hg2+ had high sensitivity, wide linear ranges, and low detection limits. The results indicated that the integration of a multi-channel structure into a paper device chips opened new opportunities for designing promising paper-based devices for metal ions diagnosis.
关键词： electrochemiluminescence,N-(4-Aminobutyl)-N-ethylisoluminol,auto-cleaning,Cu2O-Au,paper-based device,heavy metal ion
更新于2025-09-23 15:21:01
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LED&Paper-based Analytical Device for Phosphatemia/Calcemia Diagnostics
摘要： In this communication a prototype of paper-based analytical device designed for simultaneous determination of orthophosphate and calcium ions, which levels are significant for hyperphosphatemia diagnostics, is presented. The laboratory-on-paper structure for two analytes detection was wax-printed on the surface of filter paper. These two-analyte disposable paper strips are combined with two paired LED-based fluorescence detectors and simple voltmeter used as recorder of analytical signal, what makes the developed device miniature, extremely low-cost, portable and user-friendly. Thus the developed device allows usage at the patient’s bed or by patients themselves. Moreover, each paper strip is disposable and its utilization is easy and fast and, additionally, burnt strip tests ensure waste non-infectious. The presented LED&Paper-based analytical device provides low detection limits: 1.4 μM and 7.4 μM for orthophosphate and calcium ions, respectively. The practical utility of the developed device for calcemia/phosphatemia diagnostics is demonstrated using control serum standards and real human serum.
关键词： Paper-based device,Orthophosphate,Fluorescence,LEDs,Calcium
更新于2025-09-23 15:19:57
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A paper-based colorimetric microfluidic sensor fabricated by a novel spray painting prototyping process for iron analysis
摘要： A novel, simple and low-cost spray painting technique has been developed for the fabrication of microfluidic paper-based devices. The devices that we developed utilize aerosol spray paint to build hydrophobic barriers and employ a hole puncher to obtain paper-based patterned layers and paper dots without using any specialized instruments (e.g., without a laser cutter). The entire manufacturing process is extremely simple, inexpensive and rapid, which can be applied broadly. Furthermore, the application of the device to iron detection was demonstrated. A linear relationship between the color value and the iron concentration was observed from 0 to 0.02 g/L. The developed microfluidic paper-based device for iron detection exhibited a low detection limit (0.00090 g/L), good selectivity and acceptable recovery.
关键词： Spray painting,Colorimetry,Microfluidic paper-based device,Iron
更新于2025-09-19 17:15:36
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Colorimetric detection of solution concentration based on a paper-based microfluidic device
摘要： A new method for colorimetric detection of solution concentration is presented. A paper-based micro?uidic device was designed and made using printed circuit board technology. Detection zone patterns were designed and transferred to a printed circuit board by hollowing technology. The printed circuit board with patterns was stuck onto a copper sheet. The hollowed zone was ?lled with solid para?n and then covered with a piece of ?lter paper. The para?n wax was melted by heating the copper sheet and permeated into the ?lter paper. Solution to be detected was pipetted on the paper-based device with detective zones. The images of the detective zones were obtained using a microscope with charge-coupled device, which is used for calculating RGBI model to further obtain the solution concentration. Litmus was demonstrated in sample experiments. Results showed that maximum error of measured value of solution concentration to its true value is less than 2% using the presented method.
关键词： concentration,detective zone,Paper-based device,colorimetric assays
更新于2025-09-09 09:28:46