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Ratiometric fluorescence imaging for sodium selenite in living cells
摘要： Sodium selenite as a common selenium-containing agent has been extensively studied for human health, especially cancer prevention and treatment. However, precisely evaluating the dynamic changes of sodium selenite at cellular and molecular level in these pharmacological and pathophysiological studies is still challenging. Developing the chemical tools that enable the quantification of sodium selenite in vivo with high spatial and temporal fidelity thus can provide an efficient solution to this challenge. Here, we report the design, synthesis and biological evaluation of ratiometric sodium selenite fluorescent probe (HBTN-Se) for quantitative ratiometric fluorescence imaging of sodium selenite concentration fluctuations in living cells. A strategy for sodium selenite quantification has been achieved effectively through using a highly selective, rapid and biocompatible hydroxyl-deprotecting reaction. The ratiometric fluorescence imaging with HBTN-Se is constructed to precisely monitor changes in sodium selenite levels in living cells. Moreover, HBTN-Se is capable of visualizing differences in sodium selenite levels between normal cells and cancer cells, establishing the utility of this ratiometric detection platform for assessing the correlation between physiological effects of sodium selenite and its levels in living cells.
关键词： Quantitative determination,Fluorescent probe,Sodium selenite,Ratiometric fluorescence imaging
更新于2025-09-23 15:22:29
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Development of a Terbium-Sensitized Fluorescence Method for Analysis of Silibinin
摘要： Silibinin is a natural flavonoid with potent anticancer properties, as shown in both in vitro and in vivo experiments. Various methods have been used for silibinin analysis. Terbium-sensitized fluorescence methods have been widely used for the determination of drugs in pharmaceutical preparations and biological samples in recent years. The present work is aimed at providing a simple analytical method for the quantitative determination of silibinin in aqueous solutions based on the formation of a fluorescent complex with terbium ion. Terbium concentration, pH, and volume of buffer, the important effective parameters for the determination of silibinin by the proposed method, were optimized using response surface methodology. The fluorescence intensity of silibinin was measured at 545 nm using λex = 334 nm. The developed method was applied for the determination of silibinin in plasma samples after protein precipitation with acetone. Under optimum conditions, the method provided a linear range between 0.10 and 0.50 mg/L, with a coefficient of determination (R2) of 0.997. The LOD and LOQ were 0.034 and 0.112 mg/L, respectively. These results indicate that the developed method is a simple, low-cost, and suitable analytical method for the quantification of silibinin in aqueous solution and plasma samples.
关键词： Response surface methodology,Plasma samples,Quantitative determination,Silibinin,Terbium-sensitized fluorescence
更新于2025-09-23 15:21:01