Dual?Wavelength Ratiometric Electrochemiluminescence Immunosensor for Cardiac Troponin I Detection

摘要： Ratiometric electrochemiluminescence (ECL) has attracted special focus in biological analysis field because it could eliminate the environmental interference to gain precise measurement. Herein, a dual?wavelength ratiometric ECL biosensor was designed for detection of cardiac troponin I (cTnI), where (4,4’?dicarboxylicacid?2,2’?bipyridyl) ruthenium(II) (Ru(dcbpy)3 2+) and Au nanoparticles loaded graphene oxide/polyethyleneimine (GPRu?Au) nanomaterial acts as an acceptor, and Au nanoparticles modified graphitic phase carbon nitride nanosheets composite (Au?CNN) acts as donor. Au?CNN shows high and steady ECL signal centered at 455 nm, which is well matched with the adsorption of GPRu?Au, thereby a high efficient electrochemiluminescent resonance energy transfer (ECL?RET) sensing platform is designed. AuNPs facilitate the immobilization of antibody on the nanomaterials through Au?N bond. The high surface area of graphene oxide/polyethyleneimine allows a large number of Ru(dcbpy)3 2+ to be loaded, which immensely amplify the ECL signal. This sensing platform exhibits outstanding analytical performance toward cTnI with a detection limit of 3.94 fg/mL (S/N=3). The high reliability, selectivity and sensitivity of this ratiometric ECL biosensor provides a versatile sensing platform for the bioanalysis.