- 标题
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- 实验方案
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Vitamin B6 cofactors guided highly selective fluorescent turn-on sensing of histamine using beta-cyclodextrin stabilized ZnO quantum dots
摘要： Histamine, one of the most important biogenic amines (BAs) is considered as food hazard and therefore various agencies have fixed threshold in different food and beverages. In this manuscript, two novel fluorescent turn-on probes were developed for the instantaneous detection of histamine. The β-cyclodextrin (β-CD) capped ZnO quantum dots (QDs) were decorated with the vitamin B6 cofactors like pyridoxal 5′-phosphate (PLP) and pyridoxal (Py) by forming host-guest inclusion complexation between the capped β-CD and PLP/Py. The cofactors decorated QDs (ZnO@PLP and ZnO@Py) were applied for the sensing of BAs. Addition of histamine to the ZnO@PLP and ZnO@Py solution resulted selective fluorescence enhancement at 473 nm and 460 nm, respectively. Without any interference from the other tested BAs, the fluorescence response of the probes ZnO@PLP and ZnO@Py showed good linearity to histidine concentration from 2.49 to 24.4 μM and 7.44 to 47.6 μM with the detection limit down to 0.59 μM and 0.97 μM, respectively.
关键词： Vitamin B6 cofactors,ZnO QDs,Fluorescent turn-on sensor,Histamine
更新于2025-09-19 17:13:59
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Mimicking biological process to detect alkaline phosphatase activity using the vitamin B6 cofactor conjugated bovine serum albumin capped CdS quantum dots
摘要： This manuscript presents a novel bioanalytical approach for the selective ratiometric ?uorescent sensing of enzymatic activity of the alkaline phosphatase (ALP) in the biological samples. The probe was designed by conjugating the pyridoxal 5′-phosphate (PLP) over the surface of bovine serum albumin (BSA) stabilized CdS quantum dots (QDs) through the interaction of free amine present in BSA with the aldehyde group of PLP. The conjugation of PLP quenched the emission of QDs. Upon addition of the ALP, the emission of QDs was restored due to the dephosphorylation and the conversion of the functionalized PLP in to pyridoxal. With this probe, the ALP activity can be detected down to 0.05 U/L and also successfully applied for the detection of ALP activity in biological samples such as human serum and plasma.
关键词： Fluorescent sensor,Quantum dots,Vitamin B6 cofactors,Alkaline phosphatase
更新于2025-09-11 14:15:04