- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Identification of Bacteria by a Fluorescence Sensor Array based on Three Kinds of Receptors Functionalized Carbon Dots
摘要： The identification of bacteria is a crucial issue in the field of medical diagnosis, environmental monitoring and food safety. Conventional methods need long culturing times, highly skilled operators, or recognition elements specific to each kind of the bacteria. Alternatively, sensor arrays offer a cost-effective and simple approach using multiple cross-reactive receptors. Here, a facilely constructed fluorescence sensing array was developed for the identification of various bacteria. The sensor array consists of three facile synthesized carbon dots functionalized with different receptors (boronic acid, polymixin and vancomycin), which could bind to all the bacteria with different affinity instead of a specific one. A unique molecular fingerprint of each kind of bacteria was obtain from the fluorescence response pattern generated from three kinds of carbon dots. By using linear discrimination analysis (LDA) of the response pattern, the effective discrimination of six bacteria studied was demonstrated. The proposed sensor array approach may pave the way for the development of simple, rapid and effective identification of multiplex bioactive samples.
关键词： bacterial identification,cross-reactive receptors,carbon dots,pattern recognition,sensor array
更新于2025-09-19 17:15:36
-
Plasmonic Colloidosome-Based Multifunctional Platform for Bacterial Identification and Antimicrobial Resistance Detection
摘要： Antimicrobial resistance (AMR) is an urgent threat to public health. Rapid bacterial identification and AMR tests are important to promote personalized treatment of patients and to limit the spread of AMR. Herein, we explore the utility of plasmonic colloidosomes in bacterial analysis based on mass spectrometry (MS) and Raman scattering. It is found that colloidosomes can provide a rigid micron-size platform for bacterial culture and analysis. Coupled with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS, this platform enables bacterial identification at the species level with cell counts as low as 50, > 100 times more sensitive than the standard method of MALDI-TOF MS based bacterial identification. Coupled with Raman scattering, it can distinguish single bacterial cells at the strain level and recognize AMR at the single-cell level. These reveal the broad potential of the platform for flexible and versatile bacterial detection and typing.
关键词： Plasmonic colloidosomes,MALDI-TOF MS,Raman scattering,antimicrobial resistance detection,bacterial identification
更新于2025-09-11 14:15:04