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In vitro bioactivity and biocompatibility of femtosecond laser-modified Ti6Al4V alloy
摘要： The present work investigates bioactivity and biocompatibility of femtosecond (fs) laser surface-modified Ti6Al4V alloy (Ti-alloy). Self-aligned conical surface features were generated on Ti-alloy when laser irradiated employing a Ti:sapphire pulsed fs laser of wavelength 800 nm. Modification of surface chemical composition resulting from fs-laser irradiation of Ti-alloy was examined using Grazing incidence X-ray diffraction (GIXRD) technique and micro-Raman spectroscopy. Sub-oxide phase of titanium was detected on Ti-alloy surface post-fs-laser irradiation leading to increased oxygen vacancies on sample surface. For in vitro bioactivity tests, untreated and fs-laser-treated samples were immersed in simulated body fluid for 2 weeks. Evidence of hydroxyapatite deposition on both untreated Ti-alloy, as well as, fs-laser-treated Ti-alloy surfaces after in vitro tests were provided by scanning electron microscopy (SEM), energy-dispersive X-ray analysis (EDX), GIXRD, Fourier transform infrared spectroscopy (FTIR), and micro-Raman spectroscopy. Superior growth of HAP was observed on fs-laser-modified Ti-alloy surface in comparison with untreated surface. Biocompatibility of the laser-treated Ti-alloy was investigated by studying anchoring and growth of human osteosarcoma cell line (U2OS) on it. Using MTT assay technique in vitro cell viability and growth potential in the presence of untreated and laser-treated Ti-alloy samples were assessed. MTT test results demonstrated that, neither cell viability, nor growth were affected in the presence of either the untreated or laser-treated sample surfaces. In addition, in comparison with the untreated Ti-alloy surface, the fs-laser-treated Ti-alloy surface showed more efficient cellular attachment when examined under confocal microscope.
关键词： Biocompatibility,Hydroxyapatite,Surface modification,Ti6Al4V alloy,Femtosecond laser,Bioactivity
更新于2025-11-21 11:08:12
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Comparative investigation into effects of ZrO <sub/>2</sub> and Al <sub/>2</sub> O <sub/>3</sub> addition in fluorapatite laser-clad composite coatings on Ti6Al4V alloy
摘要： Composite coatings consisting of fluorapatite mixed with 20 wt% yttria (3 mol%) stabilized cubic phase zirconia (c-ZrO2, 3Y-TZP) or 20 wt% alumina (a-Al2O3) were deposited on Ti6Al4V substrates using a Nd:YAG laser cladding system. The interface morphology, phase composition, micro-hardness and biological properties of the two coatings were examined and compared. The results showed that the fluorapatite/Al2O3 specimen underwent a greater inter-diffusion at the interface between the coating layer and the transition layer than the fluorapatite/ZrO2 specimen. During the cladding process, the ZrO2 and Al2O3 components of the coating were completely decomposed or underwent phase transformation. In addition, the fluorapatite was partially decomposed. For both specimens, the coating layers contained fluorapatite, CaF2 and CaTiO3 phases. The coating layer of the fluorapatite/ZrO2 specimen additionally contained TTCP, CaO, CaZrO3 and m-ZrO2 (monoclinic phase), while that of the fluorapatite/Al2O3 specimen contained b-TCP, CaAl2O4 and u-Al2O3. The average micro-hardness of the fluorapatite/ZrO2 coating layer (1300 HV) was approximately 200 HV higher than that of the fluorapatite/Al2O3 coating layer (1100 HV). Both specimens generated dense bone-like apatite following immersion in simulated body fluid for 3 days. In other words, both specimens had a good in vitro bioactivity. However, the fluorapatite/ZrO2 specimen showed a better initial attachment and spread of osteoblast-like osteosarcoma MG63 cells than the fluorapatite/Al2O3 specimen in in vitro biocompatibility tests performed for 24 h.
关键词： Ti6Al4V,bioactivity,biocompatibility,alumina,laser cladding,Fluorapatite,zirconia
更新于2025-09-23 15:23:52
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In Vitro Bioactivity and Biocompatibility of Bio-Inspired Ti-6Al-4V Alloy Surfaces Modified by Combined Laser Micro/Nano Structuring
摘要： The bioactivity and biocompatibility play key roles in the success of dental and orthopaedic implants. Although most commercial implant systems use various surface microstructures, the ideal multi-scale topographies capable of controlling osteointegration have not yielded conclusive results. Inspired by both the isotropic adhesion of the skin structures in tree frog toe pads and the anisotropic adhesion of the corrugated ridges on the scales of Morpho butter?y wings, composite micro/nano-structures, including the array of micro-hexagons and oriented nano-ripples on titanium alloy implants, were respectively fabricated by microsecond laser direct writing and femtosecond laser-induced periodic surface structures, to improve cell adherence, alignment and proliferation on implants. The main di?erences in both the bioactivity in simulated body ?uid and the biocompatibility in osteoblastic cell MC3T3 proliferation were measured and analyzed among Ti-6Al-4V samples with smooth surface, micro-hexagons and composite micro/nano-structures, respectively. Of note, bioinspired micro/nano-structures displayed the best bioactivity and biocompatibility after in vitro experiments, and meanwhile, the nano-ripples were able to induce cellular alignment within the micro-hexagons. The reasons for these di?erences were found in the topographical cues. An innovative functionalization strategy of controlling the osteointegration on titanium alloy implants is proposed using the composite micro/nano-structures, which is meaningful in various regenerative medicine applications and implant ?elds.
关键词： bioinspired surfaces,biocompatibility,cell adherence,femtosecond laser,bioactivity,cell alignment,Ti-6Al-4V,LIPSS
更新于2025-09-19 17:13:59
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Laser Patterned ZNO Substituted Calcium Phosphate Scaffolds via Viscous Polymer Processing for Bone Graft
摘要： Polymer dough processing is a method which can easily overcome this problem. In current study ZnO doped calcium phosphate (Zn-CaP) micropatterned scaffold was explored through viscous polymer processing route. Zn-CaP nanopowder was synthesized using the wet chemical route. It was characterized using SEM, TEM, XRD and EDX. Highly ductile micro-patterned bone grafts were constructed using ZnO doped calcium phosphate scaffolds using roll milling followed by Laser machining. It was kept for cell culture and cytocompatibility using MTT, DNA quantification, and ALP assay. Hemolysis and blood cell aggregation assay was conducted for in vivo applicability. The cellular growth was also observed using fluorescence microscopy, live-dead assay, and SEM. Further gene expression was analyzed using PCR studies which showed osteogenic differentiation of bone marrow-derived stem cells without any supplemented media. The material showed a significant zone of inhibition against both gram positive and negative bacteria. Selective cellular growth was observed at micropatterned zone.
关键词： Doping,Antimicrobial activity,Bioactivity,Micropatterning,Viscous polymer processing
更新于2025-09-16 10:30:52