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Real Time Monitoring of a UV Light-Assisted Biofunctionalization Protocol Using a Nanophotonic Biosensor
摘要： A protocol for the covalent biofunctionalization of silicon-based biosensors using a UV light-induced thiol–ene coupling (TEC) reaction has been developed. This biofunctionalization approach has been used to immobilize half antibodies (hIgG), which have been obtained by means of a tris(2-carboxyethyl)phosphine (TCEP) reduction at the hinge region, to the surface of a vinyl-activated silicon-on-insulator (SOI) nanophotonic sensing chip. The response of the sensing structures within the nanophotonic chip was monitored in real time during the biofunctionalization process, which has allowed us to confirm that the bioconjugation of the thiol-terminated bioreceptors onto the vinyl-activated sensing surface is only initiated upon UV light photocatalysis.
关键词： UV light photocatalysis,biofunctionalization,silicon on insulator,nanophotonic sensor,half antibodies
更新于2025-09-23 15:23:52
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Microstructure and Characteristic of Biomedical Titanium Alloy Based on Picosecond Laser Micromachining
摘要： Laser micromachining has become a hotspot in recent years due to its high precision, non-contact and adjustable parameter. In this paper, TC4 titanium alloy implant samples were conducted to obtain specific surface textures through picosecond laser. The laser parameters which directly influenced the microstructure and characteristic of surface textures were optimized within the context of laser power, scanning speed and scanning number via response surface methodology. The microstructure was evaluated using scanning electron microscope (SEM) while the feature size of the surface textures was measured through surface 3D profiler. In addition, endothelial cell culture was conducted to investigate the biofunctionalization of samples with specific surface textures. It demonstrated that well-structured textures played an important role in promoting cell adhesion and proliferation for titanium alloy implants.
关键词： Picosecond laser micromachining,Microstructure,Biofunctionalization,Titanium alloy
更新于2025-09-23 15:22:29
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A fibrinogen biosensing platform based on plasmonic Ga nanoparticles and aminosilanea??titanate antibody trapping
摘要： The era of personalized medicine calls for rapid detection of key biomolecules, such as serum proteins, as general indicators of a healthy/sickness state. The fabrication, biofunctionalization and calibration of a fibrinogen biosensing platform based on plasmonic gallium nanoparticles is detailed in the present work. The first step consisted in the deposition of Ga nanoparticles on Si substrates by means of Joule-effect thermal evaporation technique. A distribution of nanodroplets could be evidenced by scanning electron microscopy. The biofunctionalization of the samples was carried out using aminosilane- titanate thin hybrid layers prepared by a sol-gel route. The integration of functional amino groups could be confirmed by spectroscopic methods. Then, in order to sensitize the platform surface to fibrinogen, specific immunoglobulins have been immobilized. We monitored the plasmon wavelength changes along the fabrication cascade and during detection assays with calibrated fibrinogen solutions in phosphate buffered saline. From the ellipsometric analysis, three operational ranges as a function of fibrinogen concentration can be identified, with an out of range response for low concentration, a linear response behavior in the 1-10 μM healthy range, and a tendency to saturation at high concentration.
关键词： Biosensing,biofunctionalization,Plasmonic Ga nanoparticles,Fibrinogen
更新于2025-09-23 15:21:01
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Lasera??Triggered Writing and Biofunctionalization of Thiola??Ene Networks
摘要： The light responsivity of ortho-nitrobenzyl esters (o-NBE) is exploited to inscribe μ-scale 2.5D patterns in thiol-ene networks by direct laser writing. For this purpose, a multifunctional thiol and a photosensitive alkene with an o-NBE chromophore are cured upon visible light exposure without inducing a premature photocleavage of the o-NBE links. Once the network is formed, a laser beam source with a wavelength of 375 nm is used for selectively inducing the photocleavage reaction of the o-NBE groups. Positive tone patterns are directly inscribed onto the sample surface without the requirement of a subsequent development step (removing soluble species in an appropriate organic solvent). Along with the realization of dry-developable micropatterns, the chemical surface composition of the exposed areas can be conveniently adjusted since different domains with a tailored content of carboxylic groups are obtained simply by modulating the laser energy dose. In a following step, those are activated and exploited as anchor points for attaching an Alexa-546 conjugated Protein A. Thus, the laser writable thiol-ene networks do not only provide a convenient method for the fabrication of positive tone patterns but also open future prospectives for a wide range of biosensing applications.
关键词： thiol-ene polymers,phototriggered functional groups,biofunctionalization,micropatterning,laser writing
更新于2025-09-23 15:19:57
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Patterned Surface Activation of Cyclo-Olefin Polymers for Biochip Applications
摘要： Two different surface activation methods (UV/ozone and oxygen plasma treatment) were applied for patterned surface activation of cyclo-olefin polymer (COP) surfaces combined with different masking techniques (metal shadow mask and protective tape). Surface properties were characterized by various methods such as contact angle measurement, ATR-IR, XPS and Surface enhanced ellipsometric contrast (SEEC) microscopy. UV/ozone and oxygen plasma allowed for patterned surface modification of COP leading to the formation of carboxylic and hydroxyl groups on the activated part of the surface. Stability against organic solvents was determined by rinsing the activated substrates with 2-propanol. For UV/ozone treatment it was found that a thin film of degradation products remains on the COP surface and is at least partly removed in the following washing or rinsing steps.
关键词： polymer photochemistry,Biofunctionalization,plasma modification,surface modification
更新于2025-09-09 09:28:46