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[IEEE 2018 IEEE Biomedical Circuits and Systems Conference (BioCAS) - Cleveland, OH, USA (2018.10.17-2018.10.19)] 2018 IEEE Biomedical Circuits and Systems Conference (BioCAS) - Monitoring Red Blood Cell Aggregation Dynamics in Stasis and Under Flow Using a Microfluidic Dielectric Sensor
摘要： This paper reports on monitoring the red blood cell (RBC) aggregation dynamics under flow and in static condition using a microfluidic dielectric sensor. The sensor employs a three-dimensional (3D), parallel-plate, capacitive sensing structure with a floating electrode integrated into a microfluidic channel with a height of 50μm. Using an impedance analyzer, the sensor is shown to measure the real part of complex relative dielectric permittivity of human whole blood in a frequency range of 10kHz–100MHz under flow and in static condition. The dielectric permittivity of human whole blood at 1MHz indicates the formation of RBC aggregate structures called rouleaux under static condition and their complete breakdown under a physiological shear flow rate of 500s-1. This work also demonstrates that the kinetics of RBC aggregation is dependent on fibrinogen concentration of the blood sample and establishes that the sensor is capable of distinguishing this difference in the aggregation process even under physiological shear flow rates. This work demonstrates the potential of dielectric spectroscopy in obtaining information on RBC aggregation dynamics using μL-volumes of whole blood under flow and in stasis.
关键词： red blood cells,capacitive sensor,dielectric spectroscopy,Aggregation,rouleaux formation,microfluidics
更新于2025-09-23 15:23:52
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Semi-Automatic Red Blood Cells Counting in Microscopic Digital Images
摘要： The main purpose of this study is to employ the modern technologies and techniques to semi-automate the quantification process of the Red Blood Cells in Microscopic thin blood smear digital images. The process needs to be more accurate, efficient and universal then the currently practiced methods. The study considers the process to be semi-automated for two reasons, i.e. due to the critical aspect life and due to the diverse nature of the Red Blood Cells in cluster formation. The Methodology of this study involved interactive simple cuts and morphological operations for splitting clusters of Red Blood Cells while counting is carried out through labeling matrix. The Red Blood Cells counting is part of the complete blood count test and is frequently suggested by the Physician to know the number of Red Blood Cells in the patient’s body. The proposed method considers for counting process of the Red Blood Cells first split the clusters and then count the Red Blood Cells. The proposed method achieved an overall True Positive Rate (TPR) of 0.997%, True Negative Rate (TNR) of 0.00265%, accuracy of 0.998% and average error rate of 0.001375% tested on 50 images, data set also on the same number of images linear correlation coefficient R2 is 0.997 between manual and semi-automatic counting of Red Blood Cells.
关键词： rouleaux splitting,healthcare applications,Clustered red blood cells,complete blood test,occlusions
更新于2025-09-23 15:22:29
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Stability of eosin-5'-maleimide dye used in flow cytometric analysis for red cell membrane disorders
摘要： The eosin-5'-maleimide (EMA) binding test using flow cytometry is a common method to measure reduced mean channel fluorescence (MCF) of EMA-labeled red blood cells (RBCs) from patients with red cell membrane disorders. The basic principle of the EMA-RBC binding test involves the covalent binding of EMA to lysine-430 on the first extracellular loop of band 3 protein. In the present study, the MCF of EMA was analyzed for samples derived from 12 healthy volunteers (controls) to determine the stability (i.e., the percentage decrease in fluorescence) of EMA over a period of 1 year. Comparison of periodical MCF readings over time, that is, at 2-month intervals, showed that there were no significant changes in mean channel fluorescence for up to 6 months; however, there was a significant decrease in MCF at 8 months. For optimal dye utilization, EMA remained stable only for up to 6 months. Therefore, we recommend reconstitution of the dye every 6 months when implementing this test and storage at -80oC in dark conditions.
关键词： Flow Cytometry,Red Blood cells,Hereditary spherocytosis
更新于2025-09-23 15:22:29
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Flow cytometric analysis of Xenopus laevis and X. tropicalis blood cells using acridine orange
摘要： Automated blood cell counters can distinguish cells based on their size and the presence or absence of a nucleus. However, most vertebrates have nucleated blood cells that cannot be counted automatically. We established an alternative automatic method for counting peripheral blood cells by staining cells with the fluorescent dye acridine orange (AO) and analysing cell populations using flow cytometry (FCM). As promising new animal models, we chose Xenopus laevis and three inbred strains of X. tropicalis. We compared the haematological phenotypes, including blood cell types, cell sizes, cellular structure, and erythrocyte lifespans/turnover rate among X. laevis and the three inbred strains of X. tropicalis. Each cell type from X. laevis was sorted according to six parameters: forward- and side-scattered light emission, AO red and green fluorescence intensity, and cellular red and green fluorescence. Remarkably, the erythrocyte count was the highest in the Golden line, suggesting that genetic factors were associated with the blood cells. Furthermore, immature erythrocytes in anaemic X. laevis could be separated from normal blood cells based on red fluorescence intensity. These results show that FCM with AO staining allows for an accurate analysis of peripheral blood cells from various species.
关键词： X. tropicalis,flow cytometry,acridine orange,blood cells,Xenopus laevis
更新于2025-09-23 15:21:21
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Simultaneous photoreduction and Raman spectroscopy of red blood cells to investigate the effects of organophosphate exposure
摘要： Simultaneous photoreduction and Raman spectroscopy with 532 nm laser has been used to study the effects of organophosphate (chlorpyrifos) exposure on human red blood cells. Since in red blood cells, auto-oxidation causes oxidative stress, which, in turn, is balanced by the cellular detoxicants, any possible negative effect of chlorpyrifos on this balance should results in an increased level of damaged (permanently oxygenated) hemoglobin. Therefore, when 532 nm laser, at a suitable power, was applied to photoreduce the cells, only common oxygenated form of hemoglobin got photoreduced leaving the permanently oxygenated hemoglobin detectable in the Raman spectra simultaneously excited by the same laser. Using the technique effects of chlorpyrifos to build up oxidative stress on red blood cells could be detected at concentrations as low as 10 ppb from a comparison of relative strengths of different Raman bands. Experiments performed using simultaneously exposing the cells, along with chlorpyrifos, to H2O2 (oxidative agent) and/or 3-Aminotriazole (inhibitor of anti-oxidant catalase), suggested role of chlorpyrifos to suppress the cellular anti-oxidant mechanism. Since the high level of damaged hemoglobin produced by the action of chlorpyrifos (at concentrations > 100 ppm) is expected to cause membrane damage, atomic force microscopy was used to identify such damages.
关键词： atomic force microscopy,Photoreduction,optical trap,Raman spectroscopy,red blood cells,chlorpyrifos
更新于2025-09-19 17:15:36
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[IEEE 2019 IEEE International Conference on BioPhotonics (BioPhotonics) - Taipei, Taiwan (2019.9.15-2019.9.18)] 2019 IEEE International Conference on BioPhotonics (BioPhotonics) - Deep Learning Approach for Red Blood Cell Segmentation from Full-Field OCT Data of Human Skin
摘要： The purpose of this paper is to segment red blood cells from the Full-Field OCT data of human skin, using deep learning technique. Test results show the developed technique is very promising for real time detection and counting of red blood cells.
关键词： deep learning,Segmentation,Red blood cells
更新于2025-09-12 10:27:22
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The Radioprotective Effects of Low-Intensity Laser Radiation on Rat Peripheral Blood Cells
摘要： The radioprotective effect of low-intensity laser radiation on blood cells was studied on Wistar rats. The following procedures was carried out: single total body γ-radiation (dose of 3 Gy) of rats; over-vein irradiation with continuous laser radiation (λ = 670 nm); and combined γ- and laser irradiation, which varied among different series of experiments according to the number of procedures and the energy density of the laser radiation (2.5 J/cm2, 1.25 J/cm2). The influence of γ- and laser radiation on the number of peripheral blood cells in rats, the activity of antioxidant defense enzymes (superoxide dismutase and catalase), and on the blood absorption spectra in regions sensitive to its oxygenation were studied. On the 4th day after γ-irradiation, the radioprotective effect of low-intensity laser radiation was manifested as an increase in the average number of leukocytes (up to 1.4 times) and lymphocytes (up to 1.8 times) in comparison with reduced γ-irradiation, in an increase in the relative lymphocyte proportion in the leukocyte population (up to 1.5 times), and in an increase in the activities of catalase and superoxide dismutase, which were decreased by the γ-irradiation. It was found that the changes in the number of erythrocytes, leukocytes, lymphocytes, and superoxide dismutase activity initiated by γ-radiation alone and by combined laser and γ-radiation differed in individual rats and depended on individual initial values. The molecular mechanisms of the observed photo-radiobiological effects are discussed.
关键词： low-intensity laser radiation,blood absorption spectra,γ-radiation,blood cells,antioxidant defense enzymes
更新于2025-09-11 14:15:04
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Influence of Pulsed He–Ne Laser Irradiation on the Red Blood Cell Interaction Studied by Optical Tweezers
摘要： Optical Tweezers (OT), as a revolutionary innovation in laser physics, has been extremely useful in studying cell interaction dynamics at a single-cell level. The reversible aggregation process of red blood cells (RBCs) has an important in?uence on blood rheological properties, but the underlying mechanism has not been fully understood. The regulating effects of low-level laser irradiation on blood rheological properties have been reported. However, the in?uence of pulsed laser irradiation, and the origin of laser irradiation effects on the interaction between RBCs remain unclear. In this study, RBC interaction was assessed in detail with OT. The effects of both continuous and pulsed low-level He–Ne laser irradiation on RBC aggregation was investigated within a short irradiation period (up to 300 s). The results indicate stronger intercellular interaction between RBCs in the enforced disaggregation process, and both the cell contact time and the initial contact area between two RBCs showed an impact on the measured disaggregation force. Meanwhile, the RBC aggregation force that was independent to measurement conditions decreased after a short time of pulsed He–Ne laser irradiation. These results provide new insights into the understanding of the RBC interaction mechanism and laser irradiation effects on blood properties.
关键词： red blood cells (RBCs),Helium–Neon laser,laser irradiation,optical tweezers,RBCs interactions
更新于2025-09-11 14:15:04
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[IEEE 2018 22nd International Conference on System Theory, Control and Computing (ICSTCC) - Sinaia, Romania (2018.10.10-2018.10.12)] 2018 22nd International Conference on System Theory, Control and Computing (ICSTCC) - Image Processing Techniques to Identify Red Blood Cells
摘要： This paper presents a method for the automatic identification and classification of red cells in different classes of interest for diagnosis using microscopic images of blood smear. The whole system uses different image processing techniques such as binarization, contrast enhancement, noise elimination, morphological operations (dilatation, erosion), labeling and extraction of some features of interest (area, perimeter, diameter). Using this information, some factors (form factor, circularity factor, and deviation factor) involved in the classification of red cells are calculated. The classification process has two phases: the first separates red cells in normal and abnormal type and the second classifies the abnormal in three subclasses. This system does not aim to replace the pathologist, but to assist him / her and to improve the execution time of these types of analyzes.
关键词： image processing,red blood cells,red blood cells classification,deviation factor,circularity factor
更新于2025-09-10 09:29:36
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High-speed quantitative 3D imaging by dual-illumination holographic microscopy
摘要： A new blood flow imaging (BFI) technique using digital holography with double illumination of the sample is proposed. We imaged the moving red blood cells (RBCs) using a two microscope objective lenses setup. The setup consists in a larger angle of separation (90 (cid:1)) between the two illumination beams, allowing a wider angular rotation at good z resolution. Moreover, the setup geometry allows an easier displacement of the sample in all directions. Results show that this technique is able to perform phase-shifting reconstruction for the two beams at the same time which is more suitable for the future implementation of live 3D holography. Experimental results are carried out for the verification of the effectiveness of the proposed technique on a zebrafish larvae sample.
关键词： preclinical models,red blood cells,fish embryo,holography,microcirculation
更新于2025-09-09 09:28:46