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Dose-dependent enhancement of bioactivity by surface ZnO nanostructures on acid-etched pure titanium
摘要： Zinc (Zn) is found to be essential in biologic osseous functions, and deficiency of Zn may cause delayed skeletal growth and osteoporosis. Additionally, Zn-based coatings are reported to be effective to promote the bioactivity of implants. In this study, we employed the hydrothermal treatment to incorporate Zn into the surface of acid-etched pure Ti. The process was conducted in ammonia solution with an increased Zn precursor concentration (0.0002 M, 0.002 M and 0.02 M, respectively). XPS analysis demonstrated that the nanostructures were composed of ZnO. Proliferation and alkaline phosphatase (ALP) activity of osteoblast-like SaOS-2 cells were enhanced dose-dependently, compared to those on the acid-etched pure Ti without ZnO nanostructures. This study addresses a favourable surface modification method to improve the bioactivity of implants.
关键词： nanostructure,titanium,bone regeneration,hydrothermal treatment,ZnO
更新于2025-09-23 15:23:52
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Enhancement of osteoblast activity on nanostructured NiTi/hydroxyapatite coatings on additive manufactured NiTi metal implants by nanosecond pulsed laser sintering
摘要： Background: The osteoinductive behaviors of nitinol (NiTi)-based metal implants for bone regeneration are largely dependent on their surface composition and topology. Continuous-mode laser sintering often results in complete melting of the materials and aggregation of particles, which lack control of heat transfer, as well as microstructural changes during sintering of the nanocomposite materials. Methods: In the current study, in situ direct laser deposition was used to additively manufacture three-dimensional NiTi structures from Ni and Ti powders. The mechanical property of NiTi has been shown to be similar to bone. Nanosecond pulsed laser sintering process was then utilized to generate a nanoporous composite surface with NiTi alloy and hydroxyapatite (HA) by ultrafast laser heating and cooling of Ni, Ti, and HA nanoparticles mixtures precoated on the 3D NiTi substrates; HA was added in order to improve the biocompatibility of the alloy. We then studied the underlying mechanism in the formation of NiTi/HA nanocomposite, and the synergistic effect of the sintered HA component and the nanoporous topology of the composite coating. In addition, we examined the activity of bone-forming osteoblasts on the NiTi/HA surfaces. For this, osteoblast cell morphology and various biomarkers were examined to evaluate cellular activity and function. Results: We found that the nanoscale porosity delivered by nanosecond pulsed laser sintering and the HA component positively contributed to osteoblast differentiation, as indicated by an increase in the expression of collagen and alkaline phosphatase, both of which are necessary for osteoblast mineralization. In addition, we observed topological complexities which appeared to boost the activity of osteoblasts, including an increase in actin cytoskeletal structures and adhesion structures. Conclusion: These findings demonstrate that the pulsed laser sintering method is an effective tool to generate biocompatible coatings in complex alloy-composite material systems with desired composition and topology. Our findings also provide a better understanding of the osteoinductive behavior of the sintered nanocomposite coatings for use in orthopedic and bone regeneration applications.
关键词： metal implants,pulsed laser coating,biocompatibility,differentiation,bone regeneration,alkaline phosphatase,osteoinductive behavior
更新于2025-09-23 15:22:29
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Indirect selective laser sintering printed microporous biphasic calcium phosphate scaffold promotes endogenous bone regeneration via activation of ERK1/2 signaling
摘要： Fabrication technique determines the physicochemical and biological properties of scaffold, including porosity, mechanical strength, osteoconductivity, and bone regenerative potential. Biphasic calcium phosphate (BCP)-based scaffolds are superior in bone tissue engineering due to their suitable physicochemical and biological properties. We developed an indirect selective laser sintering (SLS) printing strategy to fabricate 3D microporous BCP scaffolds for bone tissue engineering purposes. The green part of BCP scaffold was fabricated by SLS at relevantly low temperature in the presence of epoxy resin (EP) and the remaining EP was decomposed, and eliminated by a subsequent sintering process to obtain the microporous BCP scaffolds. Physicochemical properties, cell adhesion, biocompatibility, in vitro osteogenic potential and rabbit critical size cranial bone defect healing potential of the scaffolds were extensively evaluated. This indirect SLS printing eliminated the drawbacks of conventional direct SLS printing at high working temperatures, i.e., wavy deformation of the scaffold, hydroxyapatite decomposition, and conversion of β-TCP to α-TCP. Among the scaffolds printed with various binder ratios (by weight) of BCP and EP, the scaffold with 50/50 binder ratio (S4) showed the highest mechanical strength and porosity with the smallest pore size. Scaffold S4 showed the highest effect on osteogenic differentiation of precursor cells in vitro, and this effect was ERK1/2 signaling dependent. Scaffold S4 robustly promoted precursor cells homing, endogenous bone regeneration, and vascularization in rabbit critical-size cranial defect. In conclusion, BCP scaffold fabricated by indirect SLS printing maintains the physicochemical properties of BCP and possess the capacity to recruit host precursor cells to the defect site and promote the endogenous bone regeneration possibly via activation of ERK1/2 signaling.
关键词： endogenous bone regeneration,ERK1/2 signaling,biphasic calcium phosphate,selective laser sintering,bone tissue engineering
更新于2025-09-23 15:19:57
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PC458: Hyaluronic acid and laser in the therapy concept of periimplant inflammation - a 3-year clinical observation study
摘要： Anterior implant treatment is a difficult process for dental practitioner that requires experience and attention due to the patients’ high aesthetic and functional expectations. Following the loss of tooth severe hard and soft tissue alterations may take place. Regenerative techniques have been widely tested in various materials and clinical approaches; bone grafting alone, including autografts, allografts, xenografts and alloplasts; membrane alone, whether absorbable or not; and membrane in conjunction with grafting.
关键词： papilla reconstruction,implant dentistry,guided bone regeneration,peri-implant diseases
更新于2025-09-19 17:13:59
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Titanium for Consumer Applications || Bone regeneration on implants of titanium alloys produced by laser powder bed fusion: A review
摘要： Bone regeneration on biomaterials is a topic of increased interest due to the possibility of creating implants that existing bone can attach to. Titanium alloys are very suitable for this purpose and have been proven to be biocompatible, while also having suitable mechanical properties [1]. Moreover, titanium alloys are one of the most widely investigated materials for metal additive manufacturing (AM), which means that custom-designed implants can be manufactured reliably by AM. The AM technology best suited to this is powder bed fusion; laser powder bed fusion (LPBF) in particular holds some advantages. In this chapter, we investigate the current state of the art for the production of pure Ti and Ti6Al4V implants by LPBF, focusing on the requirements and capabilities for osseointegration. The first section discusses bone architecture and requirements for bone implants in general, identifying different requirements for different types of implants, and describing the various bone growth processes in more detail. This is followed by a section on surface structuring; the surface morphology and chemistry strongly influence the initial stages of bone growth, making it critical to the success of such implants. The next section describes porous structures, also known as lattice structures, which allow bone in-growth and attachment. This section describes various requirements for such lattices (pore size, lattice design, etc.) in terms of bone growth and summarizes what has been achieved thus far. The following section describes the mechanical properties of titanium lattice structures of various types that have been produced by LPBF. The next two sections describe in detail successful in vitro and in vivo experiments, respectively, for bone growth on LPBF titanium alloys. Finally, a discussion section summarizes the current state of the art and highlights requirements for future research efforts.
关键词： Bone regeneration,Titanium alloys,Laser powder bed fusion,Additive manufacturing,Osseointegration
更新于2025-09-12 10:27:22
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Post‐Treatment Engineering of Vacuum‐Deposited Cs <sub/>2</sub> NaBiI <sub/>6</sub> Double Perovskite Film for Enhanced Photovoltaic Performance
摘要： Conventional biphasic calcium phosphate (BCP) bioceramics are facing many challenges to meet the demands of regenerative medicine, and their biological properties are limited to a large extent due to the large grain size in comparison with nanocrystalline of natural bone mineral. Herein, this study aimed to fabricate porous BCP ceramic spheres with nanocrystalline (BCP-N) by combining alginate gelatinizing with microwave hybrid sintering methods and investigated their in vitro and in vivo combinational osteogenesis potential. For comparison, spherical BCP granules with microcrystalline (BCP-G) and commercially irregular BCP granules (BAM, BCP-I) were selected as control. The obtained BCP-N with specific nanotopography could well initiate and regulate in vitro biological response, such as degradation, protein adsorption, bone-like apatite formation, cell behaviors, and osteogenic differentiation. In vivo canine intramuscular implantation and rabbit mandible critical-sized bone defect repair further confirmed that nanotopography in BCP-N might be responsible for the stronger osteoinductivity and bone regenerative ability than BCP-G and BCP-I. Collectedly, due to nanotopographic similarities with nature bone apatite, BCP-N has excellent efficacy in guiding bone regeneration and holds great potential to become a potential alternative to standard bone grafts in bone defect filling applications.
关键词： nanocrystalline,calcium phosphate ceramic spheres,biological effect,bone regeneration,osteoinductivity
更新于2025-09-11 14:15:04
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Photofunctionalizing effects of hydroxyapatite combined with TiO <sub/>2</sub> on bone regeneration in rabbit calvarial defects
摘要： The hydrophilicity of bone graft material generally used as a carrier can play an important role in regulating bone morphogenetic protein (BMP) expression at the bone graft site. The hydrophilicity, altering physicochemical properties, and enhancing biological capabilities, can be increased via surface modi?cation through ultraviolet (UV) photofunctionalization and the effect on de novo osteogenesis could be further improved. Therefore, this study aimed to assess the effects of UV-irradiated TiO2-coated hydroxyapatite (HA) in combination with rhBMP-2 on bone regeneration in rabbit calvarial defects. The hydrophilicity of HA and TiO2-coated HA pellets was evaluated by measuring the contact angle of water droplets with UV irradiation. To compare de novo osteogenesis in rabbit calvarial defects, the rabbits were segregated into four different groups: negative control, HA, TiO2-coated HA, and TiO2-coated HA with UV; histomorphometric analysis and micro-computed tomography (μCT) imaging were performed after 4 and 8 weeks. In vivo analysis revealed that de novo osteogenesis occurred on the critical size defects in all groups and was signi?cantly increased in the TiO2-coated HA with UV group than in other groups (p < 0.05). The present results indicate that UV photofunctionalization promotes de novo osteogenesis.
关键词： hydroxyapatite,rabbit calvarial defects,ultraviolet,bone regeneration,titanium dioxide
更新于2025-09-04 15:30:14
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Near-infrared light control of bone regeneration with biodegradable photothermal osteoimplant
摘要： Mild heat stimulation can promote the restoration of bone defects but unfortunately, the delivery of exo-hyperthermy into human body is not efficient enough. In this study, mild heat-induced osteogenesis with high efficacy is demonstrated on an osteoimplant composed of black phosphorus nanosheets and poly(lactic-co-glycolic acid) (BPs@PLGA) with the participation of near-infrared (NIR) light irradiation. BPs@PLGA with only 0.2 wt% BPs show the highly-efficient NIR photothermal response even when being covered by a biological tissue as thick as 7 mm. In addition, this composite is completely biodegradable and the final degradation products are harmless H2O, CO2 and PO4 3- which can serve as necessary bone ingredient. The BPs@PLGA specimen mediated by low intensity and periodic NIR irradiation can effectively up-regulate the expressions of heat shock proteins and finally promote osteogenesis in vitro and in vivo. Boasting good biodegradability and NIR-mediated osteogenetic performances, the BPs@PLGA implant has great potential in orthopedic applications and this study provides new insights into the design and fabrication of new-style osteoimplants which can be remotely controlled.
关键词： black phosphorus,tissue engineering,photothermal therapy,bone regeneration
更新于2025-09-04 15:30:14