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过滤筛选
- 2018
- electromagnetic pulse
- cell proliferation
- cell membrane permeability
- cell response to electromagnetic stress
- apoptosis
- cancer therapy
- necrosis
- Intelligent Medical Engineering
- V.N. Karazin Kharkiv National University
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CHARACTERIZATION OF NEW NON-STOICHIOMETRIC FERROELECTRIC (Li0.95Cu0.15)Ta0.76Nb0.19O3 AND COMPARATIVE STUDY WITH (Li0.95Cu0.15)Ta0.57Nb0.38O3 AS PHOTOCATALYSTS IN MFCs
摘要： This work investigates the photocatalytic activity of new ferroelectric material with formula (Li0.95Cu0.15)Ta0.76Nb0.19O3 (LT76) in a single chamber microbial fuel cell (MFC) and compare its performance with the similar photocatalyst (Li0.95Cu0.15)Ta0.57Nb0.38O3 (LT57). The photocatalysts LT76 and LT57 were synthesized by ceramic route under the same conditions, with the same starting materials. The ratio Ta/Nb was fixed at 4.0 and 1.5 for LT76 and LT57 respectively. These phases were characterized by different techniques including X-Ray diffraction (XRD), transmission electronic microscopy (TEM), particle size distribution (PSD), differential scanning calorimetric (DSC) and UV-Vis. The new photocatalyst LT76 presents specific surface area about 0.791m2/g and Curie temperature of 1197°C. The photocatalytic efficiency of this material is evaluated in terms of wastewater treatment and electricity generation by power density and removal rate of chemical oxygen demand (COD) in the presence of a light source. The values of the maximum power densities and COD removal were 19.77 mW/m3 and 93% respectively for LT76.
关键词： microbial fuel cell,non-stoichiometry,ferroelectric,Lithium niobate-tantalate,photocatalyst
更新于2025-09-09 09:28:46
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Reporter Genes for PET Imaging of CAR-T Cells Offers Insight into Adoptive Cell Transfer
摘要： Immunotherapy has drastically altered cancer treatment over the past decade. Several strategies have emerged to successfully harness the immune system as a therapeutic instrument. In lymphoma, an adoptive cell transfer approach using chimeric antigen receptor (CAR) T cells has received FDA approval for treatment in non-Hodgkin lymphoma and diffuse large B-cell lymphoma (1). In solid tumors, checkpoint blockade of PD-1 and CTLA-4 have significantly increased durable remissions in a number of late-stage diseases, most notably melanoma and non-small cell lung cancer (2,3). Although checkpoint blockade has demonstrated more success in solid tumors to date, a significant focus has been placed on the adaptation of CAR T cell therapy for solid tumors. Dozens of trials investigating a mixture of potential targets including EGFR, HER2, mucins, EPCAM and other antigens are actively under clinical investigation. While the promise of rationally designing a patient’s own immune cells to specifically eliminate tumors is promising, CAR T therapy also can pose significant risk of severe side effects or patient death. Given the great promise and risk of such a therapy, a method to actively monitor the number of viable CAR T cells and their biodistribution is of great importance.
关键词： Adoptive Cell Transfer,Immunotherapy,Imaging,CAR-T
更新于2025-09-09 09:28:46
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Highly Efficient Near-Infrared Photothermal Conversion of a Single Carbon Nanocoil Indicated by Cell Ejection
摘要： Carbon nanocoil (CNC), which has excellent properties of near-infrared (NIR) absorption and photo-electrical response, is considered as a potential NIR sensing and bioengineering material. In this work, a high-speed living cell ejection is realized in a yeast cell solution based on the photothermal conversion induced by a NIR laser irradiation on the surface of a CNC. The cell ejection reveals a thermal convection of solution induced by the laser irradiated CNC and can be used to evaluate the photothermal conversion ability of CNC. The dynamic behavior of the thermal convection behind cell ejection is studied experimentally and analytically. It is found that the initial solution flow velocity of the dynamic process reaches more than 103 μm/s. Approximately, 60% of the laser energy illuminated on the CNC is converted into thermal energy. The unique helical morphology of CNC enables its high NIR photothermal conversion efficiency. The average convective heat transfer coefficient on the contact area between CNC and surrounding water is deduced to be as high as 7.0 × 105 W/(m2·K). These results indicate that CNC has promising potential applications on microfluidics, laser-operated flow cytometers, bioparticle ejection, and micro-/nano-laser-operated heat generators and exchangers.
关键词： Cell ejection,Thermal convection,Carbon nanocoil,Photothermal conversion,Near-infrared
更新于2025-09-09 09:28:46
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Enhancement of Luminescence Intensity in Dy3+ Ions Doped YVO4 Nanomaterials by Ba2+ Ion Codoping and YVO4:2Dy/Fe3O4 Nanohybrid for Hyperthermia Application
摘要： The enhancement of the luminescence intensity of the Dy3+ by Ba2+ ions (at different concentrations) codoping into YVO4:2Dy nanoparticles at different annealing temperature of as-prepared, at 500 and 900°C were studied. The XRD study shows the strains are induced when dopant and codopant are incorporated into host matrix. XRD patterns of nanohybrids show the existence of two phases corresponding to YVO4:2Dy and Fe3O4 nanoparticles. TEM image of YVO4:2Dy nanoparticles were spherical shape whereas for YVO4:2Dy/Fe3O4 nanohybrids, the spherical particles are forming chain like structure due to the PEG molecule which binds both YVO4:2Dy and Fe3O4 during nanohybrids formation. Increase in absorption coefficient and decrease in lattice strain values are the major reasons for the enhancement of emission intensity in photoluminescent (PL). Lifetime studies also shows correlation between the calculated energy transfer and diffusion for indirect excitation which is also studied here. High quantum efficiency up to 45% can be achieved. YVO4:2Dy/Fe3O4 nanohybrids is found to achieve hyperthermia temperature 42°C in short time. This nanohybrids shows luminescence in the region of biological window indicating nanohybrids can be used as bioimaging probe. It also shows high viability up to 94% in HeLa cancer cells.
关键词： Vanadate nanoparticles,HeLa cell,Nanohybrids,Enhancement of luminescence,Hyperthermia
更新于2025-09-09 09:28:46
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Fast confocal fluorescence imaging in freely behaving mice
摘要： Fluorescence imaging in the brain of freely behaving mice is challenging due to severe miniaturization constraints. In particular, the ability to image a large field of view at high temporal resolution and with efficient out-of-focus background rejection still raises technical difficulties. Here, we present a novel fiberscope system that provides fast (up to 200 Hz) background-free fluorescence imaging in freely behaving mice over a field of view of diameter 230 μm. The fiberscope is composed of a custom-made multipoint-scanning confocal microscope coupled to the animal with an image guide and a micro-objective. By simultaneously registering a multipoint-scanning confocal image and a conventional widefield image, we subtracted the residual out-of-focus background and provided a background-free confocal image. Illumination and detection pinholes were created using a digital micromirror device, providing high adaptability to the sample structure and imaging conditions. Using this novel imaging tool, we demonstrated fast fluorescence imaging of microvasculature up to 120 μm deep in the mouse cortex, with an out-of-focus background reduced by two orders of magnitude compared with widefield microscopy. Taking advantage of the high acquisition rate (200 Hz), we measured red blood cell velocity in the cortical microvasculature and showed an increase in awake, unrestrained mice compared with anaesthetized animals.
关键词： red blood cell velocity,multipoint-scanning confocal microscope,digital micromirror device,fiberscope system,microvasculature imaging,fluorescence imaging,freely behaving mice
更新于2025-09-09 09:28:46
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Recent Advances in Cell Membrane-Camouflaged Nanoparticles for Cancer Phototherapy
摘要： Phototherapy including photothermal therapy (PTT) and photodynamic therapy (PDT) employs phototherapeutic agents to generate heat or cytotoxic reactive oxygen species (ROS), and has therefore garnered particular interest for cancer therapy. However, the main challenges faced by conventional phototherapeutic agents include easy recognition by the immune system, rapid clearance from blood circulation, and low accumulation in target sites. Cell-membrane coating has emerged as a potential way to overcome these limitations, owing to the abundant proteins on the surface of cell membranes that can be inherited to the cell membrane–camouflaged nanoparticles. This review summarizes the recent advances in the development of biomimetic cell membrane–camouflaged nanoparticles for cancer phototherapy. Different sources of cell membranes can be used to coat nanoparticles using different coating approaches. After cell-membrane coating, the photophysical properties of the original phototherapeutic nanoparticles remain nearly unchanged; however, the coated nanoparticles are equipped with additional physiological features including immune escape, in vivo prolonged circulation time, or homologous targeting, depending on the cell sources. Moreover, the coated cell membrane can be ablated from phototherapeutic nanoparticles under laser irradiation, leading to drug release and thus synergetic therapy. By combining other supplementary agents to normalize tumor microenvironment, cell-membrane coating can further enhance the therapeutic efficacy against cancer.
关键词： biomimetic nanoparticles,photodynamic therapy,cell membrane,photothermal therapy
更新于2025-09-09 09:28:46
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Identification of Bacterial Diseases in Rice Plants Leaves by the Use of Spectroscopic Imaging
摘要： Rice is staple in the African habitats menu. Bacterial wilt (BLB) and leaf streak (BLS) are some of the phytopathological diseases which restrain rice production around the world. In this paper, multi-spectral and multi- modal imaging techniques have been developed to characterize the rice leaves with symptoms of bacterial wilt (BLB) and leaf streak (BLS), and to provide information on their effects, in order to reduce their spread. First, we recorded microscopic and spectroscopic images of the samples using multimodal and multispectral microscope, with spectral region ranging from UV to NIR, for each mode. Then, we extracted the spectral footprints of the cells constituents, in transmission, reflection and scattering from the spectral images. Applying multivariate statistical analysis methods to this optical spectra allowed us to characterize the effect of bacterial rice leaves caused by Xanthomonas oryzae strains. The results of the proposed technique can be useful for easy identification of this type of infection, and can serve as routine approach in biochemical and agronomic laboratories.
关键词： Xanthomonas oryzae,multispectral and multimodal microscopy,spectroscopic imaging,plant cell diseases,classification,rice
更新于2025-09-09 09:28:46
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Nano- and Microfabrication for Industrial and Biomedical Applications || Chemical and biological sensors at component and?device level
摘要： Chemical and biological sensors were initially explored at the microscale as an extension of microchip technology. They have considerable advantages because of their rapid throughput, and the need for very small samples. The development of better and smaller photometric and fluorometric detectors has enabled optical absorbance systems to be produced on a chip. Electrochemical sensors measure the conductance of electrolytes in an aqueous sample, and can also measure concentrations of gases that diffuse into the sample. This makes them valuable as personal health care monitors. These systems have been applied in medical monitoring, and for monitoring and controlling industrial-scale reactions. The integrated chemFET device is an example of a real-world application of this technology, and measures pH. The device is based on the ion-sensitive field-effect transistor (ISFET), which is caused by ions in solution producing a current across a transistor. Changes in the pH produce a proportionate change in the signal. Biological material, including DNA and cells, can also be used as a sensor. Cells have been used to detect pathogens in food, by adhering them to a sensor’s surface by means of a polymer, and then detecting changes in their electrochemistry. DNA optical readout is a widely accepted method for analyzing DNA sequences.
关键词： pH measurement,Biological sensors,Microchip technology,Electrochemical sensors,Chemical sensors,ISFET,Cell-based biosensors,Optical sensors,DNA analysis
更新于2025-09-09 09:28:46
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[IEEE 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018) - Washington, DC (2018.4.4-2018.4.7)] 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018) - Segmentation of cell nuclei using intensity-based model fitting and sequential convex programming
摘要： We introduce a convex model-based approach for the segmentation of cell nuclei, which exploits both shape and intensity information. The model is directly fitted to the image intensities. Previous shape-based approaches either are not globally optimal or require prior binarization of an image. Our approach relies on a fast second-order optimization scheme to solve a sequence of convex programs and estimate the globally optimal solution based on the image intensities. Model fitting is performed within image regions which are determined by exploiting the local image structure. We evaluated our approach using fluorescence microscopy images of two different cell types and performed a quantitative comparison with previous methods.
关键词： model fitting,Fluorescence microscopy,convex optimization,cell segmentation
更新于2025-09-09 09:28:46
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Amino-substituted C-coumarins: Synthesis, spectral characterizations and their applications in cell imaging
摘要： Compact fluorophores are desired for bioconjugation because they minimally perturb the native biological functions of proteins or organelles. However, their absorption and emission wavelengths are typically short and not ideal for practical applications. For example, coumarin is one of the smallest fluorophores with excellent fluorescence properties. However, its potentials in biological applications are limited by its relatively short excitation wavelengths in the UV spectral region. It is the aim of this manuscript to develop coumarin analogs absorbing and emitting in the visible range through rational molecular engineering. We designed and synthesized C-coumarin dyes, and found that their maximal absorption wavelengths fall well within the visible region, rendering them superior for live cell imaging than O-coumarins. The other promising properties of C-coumarins include larger Stokes’ shifts, low cytotoxicity and high chemostability.
关键词： cell imaging,C-coumarins,synthesis,spectral characterizations
更新于2025-09-09 09:28:46