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- 摘要
- 关键词
- 实验方案
- 产品
过滤筛选
- 2018
- electromagnetic pulse
- cell proliferation
- cell membrane permeability
- cell response to electromagnetic stress
- apoptosis
- cancer therapy
- necrosis
- Intelligent Medical Engineering
- V.N. Karazin Kharkiv National University
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Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
摘要： Purpose: To compare cryopreserved human corneal endothelial cells (HCECs) grown in human serum-supplemented media (HS-SM) with cryopreserved HCECs grown in fetal bovine serum-supplemented media (FBS-SM). Methods: Three pairs of human corneas from donors aged 8, 28, and 31 years were obtained from the eye bank. From each pair, one cornea was used to start a HCEC culture using HS-SM; the other cornea was grown in FBS-SM. On reaching confluence, the six cell populations were frozen using 10% dimethyl sulfoxide-containing medium. Thawed cells grown in HS-SM were compared with those grown in FBS-SM with respect to morphology, growth curves, immunohistochemistry, real time-reverse transcriptase polymerase chain reaction (RT-PCR) for endothelial cell markers, and detachment time. Results: No difference in morphology was observed for cells grown in the two media before or after cryopreservation. By growth curves, cell counts after thawing were similar in both media, with a slight trend toward higher cell counts in FBS-SM. Cells grown in both the media demonstrated a similar expression of endothelial cell markers when assessed by immunohistochemistry, although HCEC marker gene expression was higher in cells grown in HS-SM than in those grown in FBS-SM as assessed by RT-PCR. With FBS-SM, there was a tendency of longer detachment time and lower cell passages. Conclusions: HS-SM was similar to FBS-SM for cryopreservation of cultured HCECs as assessed by analysis of cell morphology, proliferation, and protein expression, although marker gene expression was higher in cells grown in HS-SM than in those grown in FBS-SM. Detachment time was longer with FBS-SM and in lower passages.
关键词： Cell culture techniques,Culture media,Endothelium, Corneal,Cornea,Cryopreservation
更新于2025-09-09 09:28:46
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Cascaded Electrochemiluminescence Signal Amplifier for the Detection of Telomerase Activity from Tumor Cells and Tissues
摘要： Telomerase is closely linked to the physiological transformation of tumor cells and is commonly overexpressed in most types of tumor cells. Therefore, telomerase has become a potential biomarker for the process of tumorigenesis, progression, prognosis and metastasis. Thus, it is important to develop a simple, accurate and reliable method for detecting telomerase activity. As a high signal-to-noise ratio mode, electrochemiluminescence (ECL) has been widely applied in the field of biomedical analysis. Here, our objective was to construct an improved ECL signal amplifier for the detection of telomerase activity. Methods: A cascaded ECL signal amplifier was constructed to detect telomerase activity with high selectivity via controllable construction of a lysine-based dendric Ru(bpy)3 2+ polymer (DRP). The sensitivity, specificity and performance index were simultaneously evaluated by standard substance and cell and tissue samples. Results: With this cascaded ECL signal amplifier, high sensitivities of 100, 50, and 100 cells for three tumor cell lines (A549, MCF7 and HepG2 cell lines) were simultaneously achieved, and desirable specificity was also obtained. Furthermore, the excellent performance of this platform was also demonstrated in the detection of telomerase in tumor cells and tissues. Conclusion: This cascaded ECL signal amplifier has the potential to be a technological innovation in the field of telomerase activity detection.
关键词： telomerase activity,signal amplifier,tumor cell,tumor tissue
更新于2025-09-09 09:28:46
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Optimized Electrolyte Loading and Active Film Thickness for Sandwich Polymer Light-Emitting Electrochemical Cells
摘要： Effects of ion concentration and active layer thickness play a critical role on the performance of light-emitting electrochemical cells. Expanding on a pioneering materials system comprising the super yellow (SY) polymer, and the electrolyte trimethylolpropane ethoxylate (TMPE)/Li+CF3SO3?, it is reported that a slightly lowered salt concentration and layer thickness result in a substantial efficiency increase, and that this increase is confined to a narrow concentration and thickness range. For a film thickness of 70 nm, a blend ratio SY:TMPE:Li+CF3SO3? = 1:0.075:0.0225, and a current of 7.7 mA cm?2 the current efficacy is 11.6 cd A?1, on a par with SY light-emitting diodes. The optimized salt content can be explained by increased exciton quenching at higher concentrations and hindered carrier injection and conduction at lower concentrations, while the optical dependence on the layer thickness is due to weak microcavity effects. A comprehensive optical modeling study is presented, which includes the doping-induced changes of the refractive indices and self-absorption losses due the emission–absorption overlap of intrinsic and doped SY. The analysis indicates either a thickness-independent emitter position (EP) close to the anode or a thickness-dependent EP, shifted to the cathode for increased thicknesses.
关键词： electrolyte,super yellow,light-emitting electrochemical cell,LEC,optical model
更新于2025-09-09 09:28:46
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Facile Synthesis of Resveratrol Nanogels with Enhanced Fluorescent Emission
摘要： Herein, a kind of fluorescent resveratrol nanogels via one-pot thiol-ene Michael addition polymerization of resveratrol triacrylate, 1,6-hexanedithiol, and methoxyl poly(ethylene glycol) acrylate is prepared. The resultant nanogels can be well-dispersed in water with a hydrodynamic radius of around 68 nm, and the nanogels are stable in both water and organic solvents. Moreover, the resveratrol nanogels exhibit elevated fluorescence intensity compared to free resveratrol, and the quantum yield of resveratrol nanogels is estimated to be 5.8 times as that of free resveratrol dispersed in water. Fluorescence image results also demonstrate that the resveratrol nanogels can be used for cell imaging in MCF-7 human breast cancer cells. Therefore, the resveratrol nanogels are expected to be used as a trackable drug delivery system.
关键词： resveratrol,cell imaging,thiol-ene,nanogel,enhanced fluorescence
更新于2025-09-09 09:28:46
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30.2: Amorphous Silicon Thin-Film Transistor Gate Driver Circuit Design with Time Division Driving Method for In-Cell Touch Display Panel
摘要： In this paper, an integrated hydrogenated amorphous silicon (a-Si:H) thin-film transistor (TFT) gate driver circuit design with the time division driving method is demonstrated for in-cell touch display panels. The design can avoid long-term biasing of the pull-up TFT in the conventional gate driver circuits and address the issues due to leakage current at Q node in the circuit during the touch sensing period. Therefore, the overall reliability of the circuit is improved. The design of the circuit is also simple without needing any additional signal inputs. As a result, the proposed circuit design can be used to pause display operation to perform touch sensing operation several times per frame, which can realize 60 Hz display scanning and 120 Hz touch reporting rate.
关键词： GOA,Time division driving,Gate driver on array,In-cell touch
更新于2025-09-09 09:28:46
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A FRET-based ratiometric fluorescent probe for detection of intrinsically generated SO2 derivatives in Mitochondria
摘要： A mitochondria-targeted ratiometric fluorescent probe based on hemicyanine and pyrido[1,2-a]benzimidazole was presented. It shows high sensitivity and selectivity toward SO2 in pure water. The limit of detection (LOD) was as low as 26.7 nM, which is superior to most reported probes. Most importantly, the probe was successfully used for fluorescence imaging of endogenous bisulfite in mitochondria in Glioma cells.
关键词： pyrido[1,2-a]benzimidazole,water,cell imaging,hemicyanine,mitochondria
更新于2025-09-09 09:28:46
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Two-dimensional porphyrin-based covalent organic framework: A novel platform for sensitive epidermal growth factor receptor and living cancer cell detection
摘要： A porphyrin-based covalent organic framework (denoted as p-COF) was synthesized by a simple oil-bath method and exploited as a novel sensing layer for immobilizing epidermal growth factor receptor (EGFR)-targeting aptamer strands to detect trace EGFR and living michigan cancer foundation-7 (MCF-7) cells for the first time. p-COF presented a nanosheet-like structure, large cavities, rich nitrogen-bearing groups, high electrochemical activity, excellent bioaffinity, low toxicity, and good stability in aqueous solution; the microstructural features of this material enabled strong immobilization of the aptamer strands. Interactions between the aptamer strands and EGFR significantly changed the electrochemical signals of the modified electrode due to the formation of an aptamer-EGFR complex. The p-COF-based aptasensor exhibited an extremely low detection limit (LOD) of 5.64 fg?mL?1 obtained from differential pulse voltammetry and 7.54 fg?mL?1 originated from electrochemical impedance spectroscopy with a broad linear detection range of 0.05–100 pg?mL?1 of the EGFR concentration. When detecting living MCF-7 cells, the p-COF-based aptasensor showed an LOD of 61 cell?mL?1 with a linear detection range of 500 × 105 cell?mL?1. The fabricated aptasensor exhibited high selectivity, good stability, reproducibility, acceptable recyclability, and favorable applicability in human serum samples. We believe that the developed p-COF-based aptasensor is a potential candidate for the sensitive detection of target cancer markers or living cells.
关键词： Detection of epidermal growth factor receptor,Living cancer cell detection,Porphyrin-based covalent organic framework,Electrochemical aptasensor
更新于2025-09-09 09:28:46
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Quantitative analysis of the retinal nerve fiber layer, ganglion cell layer and optic disc parameters by the swept source optical coherence tomography in patients with migraine and patients with tension-type headache
摘要： The aim of the study was to measure the thicknesses of the inner retinal segments and optic nerve head (ONH) parameters in migraineurs and patients with tension-type headache (TTH) in headache-free period using swept source optical coherence tomography (SS-OCT) and to compare the outcomes with each other and those of healthy subjects. The study population consisted of 23 migraineurs, 22 TTH patients, and 25 controls with a best-corrected visual acuity of 20/20 and without a history of systemic or ocular disease. Macular ganglion cell inner plexiform layer (mGCIPL), macular ganglion cell complex (mGCC), circumpapillary retinal nerve fiber layer (cpRNFL), and ONH parameters were evaluated using SS-OCT, and the areas under the receiver-operating characteristic (ROC) curves were calculated to determine the ability of these parameters to distinguish between the patient and normal eyes. There were not statistically significant differences between the measurements acquired from migraineurs, TTH patients, and the controls. The outcomes of the patients with TTH were very similar to those of the normal participants. The areas under the ROC curves (AUC) correlated highly with the measurements obtained from the same subfields for the mGCC, MGCIPL, cpRNFL, and ONH parameters. In conclusion, SS-OCT presented reproducible and reliable measurements of posterior segment layers of the eyes, especially in sectoral configuration, and the parameters did not show significant difference between the groups.
关键词： Migraine,Swept source optical coherence tomography,Retinal nerve fiber layer,Ganglion cell layer,Tension-type headache
更新于2025-09-09 09:28:46
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A synergistic heterostructured ZnO/BaTiO3 loaded carbon photoanode in photocatalytic fuel cell for degradation of Reactive Red 120 and electricity generation
摘要： Photocatalytic fuel cell (PFC) is considered as a sustainable green technology which could degrade organic pollutant and generate electricity simultaneously. A synergistic double-sided ZnO/BaTiO3 loaded carbon plate heterojunction photoanode was fabricated in different ratios by using simple ultrasonication and mixed-annealed method. The double-sided design of photoanode allowed the lights irradiated at both sides of the photoanode. The ferroelectricity fabricated photoanode was applied in a membraneless PFC with platinum-loaded carbon as the cathode. Results revealed that the photoanode with 1:1 ratio of BaTiO3 and ZnO exhibited a superior photocatalytic activity among all the photoanodes prepared in this study. The heterojunction of this photoanode was able to achieve up to a removal efficiency of 93.67 % with a maximum power density of 0.5284 μW cm-2 in 10 mg L-1 of Reactive Red 120 (RR120) without any supporting electrolyte. This photoanode was able to maintain at high performance after recycling 3 times. Over loading of ZnO above 50% on BaTiO3 could lead to deterioration of the performance of PFC due to the charge defects and light trapping ability. The interactions, interesting polarizations of the photocatalysts and proposed mechanism of the n-n type heterojunction in the photoanode of ZnO/BaTiO3 was also discussed.
关键词： Photocatalytic fuel cell,Degradation,Reusability,ZnO/BaTiO3 heterojunction,Electricity Generation
更新于2025-09-09 09:28:46
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A Facile Strategy for Preparation and Application of Squaraine Nanometer Hybrids with Broad Band Absorption
摘要： Organic materials are receiving more attention in the solar cell due to their better solution process, controllable synthesis, tunable chemical and physical properties. However, the focus problem of compatibility and aggregation is to be solved urgently in organic materials, which inevitably limits them widely application. To this end, an inorganic-organic hybrid materials with wide-band absorption is designed and prepared, that incorporates octavinyl-polyhedral oligomeric silsesquioxane with 6-bromoquanaldine to produce H1, then reacts with squaric acid to design H2, which efficiently avoids the fluorescence quenching due to the steric hindrance. The electrochemical results shows that the energy level of H2 is match well with the commercial dye N719. Hence it shows a better photovoltaic performance based on dye-sensitized solar cell, along with power conversion efficiency up to 7.73 % when it is co-sensitized with N719. The hybrid system exhibits good performances in solar cell and long-term stability.
关键词： dye-sensitized solar cell,disaggregation,broad-band absorption,co-sensitizer
更新于2025-09-09 09:28:46