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过滤筛选
- 2018
- electromagnetic pulse
- cell proliferation
- cell membrane permeability
- cell response to electromagnetic stress
- apoptosis
- cancer therapy
- necrosis
- Intelligent Medical Engineering
- V.N. Karazin Kharkiv National University
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Enhancements of Cancer Cell Damage Efficiencies in Photothermal and Photodynamic Processes through Cell Perforation and Preheating with Surface Plasmon Resonance of Gold Nanoring
摘要： The methods of cell perforation and preheating are used for increasing cell uptake ef?ciencies of gold nanorings (NRIs), which have the localized surface plasmon resonance wavelength around 1064 nm, and photosensitizer, AlPcS, and hence enhancing the cell damage ef?ciency through the photothermal (PT) and photodynamic (PD) effects. The perforation and preheating effects are generated by illuminating a defocused 1064-nm femtosecond (fs) laser and a defocused 1064-nm continuous (cw) laser, respectively. Cell damage is produced by illuminating cell samples with a focused 1064-nm cw laser through the PT effect, a focused 1064-nm fs laser through both PT and PD effects, and a focused 660-nm cw laser through the PD effect. Under various conditions with and without cell wash before laser illumination, through either perforation or preheating process, cell uptake and hence cell damage ef?ciencies can be enhanced. Under our experimental conditions, perforation can be more effective at enhancing cell uptake and damage when compared with preheating.
关键词： cell perforation,photothermal therapy,photodynamic therapy,cell preheating,surface plasmon resonance
更新于2025-09-04 15:30:14
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Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices
摘要： A recent clinical trial proves that autologous olfactory mucosal cell (OMC) transplantation improves locomotion in dogs with naturally occurring spinal injuries comparable to human lesions. However, not all dogs respond to the treatment, likely due to the transplantation procedures involving injections of cell suspensions that are associated with cell death, uneven cell distribution, and cell washout. Encapsulating cells in protective hydrogel matrices offers a tissue engineering solution to safely achieve 3D growth of viable transplant cells for implantation into injury sites, to improve regenerative outcomes. It is shown for the first time that canine OMCs (cOMCs) can be propagated with high viability in 3D collagen matrices. Further, a method to incorporate cOMCs pre-labeled with clinical-grade iron oxide nanoparticles into the constructs is described. Intraconstruct labeled cells are visualized using magnetic resonance imaging, offering substantial promise for in vivo tracking of cOMCs delivered in protective matrices.
关键词： magnetic nanoparticle,canine olfactory mucosal cell,cell transplantation,hydrogel,spinal injury
更新于2025-09-04 15:30:14
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Sorting and manipulation of biological cells and the prospects for using optical forces
摘要： Contemporary biomedical research requires development of novel techniques for sorting and manipulation of cells within the framework of a microfluidic chip. The desired functions of a microfluidic chip are achieved by combining and integrating passive methods that utilize the channel geometry and structure, as well as active methods that include magnetic, electrical, acoustic and optical forces. Application of magnetic, electric and acoustics-based methods for sorting and manipulation have been and are under continuous scrutiny. Optics-based methods, in contrast, have not been explored to the same extent as other methods, since they attracted insufficient attention. This is due to the complicated, expensive and bulky setup required for carrying out such studies. However, advances in optical beam shaping and computer hardware, and software have opened up new opportunities for application of light to development of advanced sorting and manipulation techniques. This review outlines contemporary techniques for cell sorting and manipulation, and provides an in-depth view into the existing and prospective uses of light for cell sorting and manipulation.
关键词： Cell manipulation,Optical sorting,Microfluidics,Acoustic sorting,Cell separation,Dielectrophoresis,Magnetic manipulation
更新于2025-09-04 15:30:14
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Proton versus photon radiation-induced cell death in head and neck cancer cells
摘要： Background: Photon (X-ray) radiotherapy (XRT) kills cells via DNA damage, however, how proton radiotherapy (PRT) causes cell death in head and neck squamous cell carcinoma (HNSCC) is unclear. We investigated mechanisms of HNSCC cell death after XRT versus PRT. Methods: We assessed type of death in 2 human papillomavirus (HPV)-positive and two HPV-negative cell lines: necrosis and apoptosis (Annexin-V fluorescein isothiocyanate [FITC]); senescence (β-galactosidase); and mitotic catastrophe (γ-tubulin and diamidino-phenylindole [DAPI]). Results: The XRT-induced or PRT-induced cellular senescence and mitotic catastrophe in all cell lines studied suggested that PRT caused cell death to a greater extent than XRT. After PRT, mitotic catastrophe peaked in HPV-negative and HPV-positive cells at 48 and 72 hours, respectively. No obvious differences were noted in the extent of cell necrosis or apoptosis after XRT versus PRT. Conclusion: Under the conditions and in the cell lines reported here, mitotic catastrophe and senescence were the major types of cell death induced by XRT and PRT, and PRT may be more effective.
关键词： photon radiotherapy,radiation-induced cell death,head and neck cancer,cell death mechanisms,proton radiotherapy
更新于2025-09-04 15:30:14
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[IEEE 2018 7th International Conference on Renewable Energy Research and Applications (ICRERA) - Paris, France (2018.10.14-2018.10.17)] 2018 7th International Conference on Renewable Energy Research and Applications (ICRERA) - Effective Series-Parallel Cell Configuration in Solar Panels for FPM Power Generation Forest
摘要： Three-dimensional (3D) Fibonacci number photovoltaic modules (FPMs), which are designed in part based on natural plant leaf phyllotaxis, have previously been proposed as a means of efficiently collecting solar energy over the course of a day. When multiple FPMs are installed in the same area, they can be arranged like trees in a forest. As part of efforts to realize such power generation forests, this study uses simulations and experiments to clarify the relationship between the cell configurations within a solar cell panel and the power generation that occurs when an adjacent FPM is installed.
关键词： shadow,FPM,solar cell module,3-dimensional photovoltaic module,cell configuration,power generation forest
更新于2025-09-04 15:30:14
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Brahma deficiency in keratinocytes promotes UV carcinogenesis by accelerating the escape from cell cycle arrest and the formation of DNA photolesions
摘要： Background: Ultraviolet radiation (UVR) is the principal cause of keratinocyte skin cancers. Previous work found that levels of the chromatin remodelling protein, Brahma (Brm), are diminished during the progression from actinic keratoses to cutaneous squamous cell carcinomas in humans, and its loss in UV-irradiated mouse skin causes epidermal hyperplasia and increased tumour incidence. Methods: The skins of mice and mouse and human keratinocytes deficient in Brm were exposed to UVR and evaluated for cell cycle progression and DNA damage response. Objective: To identify the mechanisms by which loss of Brm contributes to UVR-induced skin carcinogenesis. Results: In both mouse keratinocytes and HaCaT cells, Brm deficiency led to an increased cell population growth following UVR exposure compared to cells with normal levels of Brm. Cell cycle analysis using a novel assay showed that Brm-deficient keratinocytes entered cell cycle arrest normally, but escaped from cell cycle arrest faster, enabling them to begin proliferating earlier. In mouse keratinocytes, Brm primarily affected accumulation in G0/G1-phase, whereas in HaCaT cells, which lack normal p53, accumulation in G2/M-phase was affected. Brm-deficient keratinocytes in mouse skin and human cell cultures also had higher levels of UVR-induced cyclobutane pyrimidine dimer photolesions. These effects occurred without any compensatory increase in DNA repair or cell death to remove photolesions or the cells that harbor them from the keratinocyte population. Conclusion: The loss of Brm in keratinocytes exposed to UVR enables them to resume proliferation while harboring DNA photolesions, leading to an increased fixation of mutations and, consequently, increased carcinogenesis.
关键词： SWI/SNF,Cutaneous squamous cell carcinoma,UV radiation,Cell cycle arrest,DNA damage
更新于2025-09-04 15:30:14
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Ability of Swept-Source Optical Coherence Tomography to Detect Retinal and Choroidal Changes in Patients with Multiple Sclerosis
摘要： Purpose. To evaluate the ability of new swept-source (SS) optical coherence tomography (OCT) technology to detect changes in retinal and choroidal thickness in patients with multiple sclerosis (MS). Methods. A total of 101 healthy and 97 MS eyes underwent retinal and choroidal assessment using SS Triton OCT (Topcon). Macular thickness and peripapillary data (retinal, ganglion cell layer (GCL+, GCL++) and retinal nerve fiber layer (RNFL) thickness) were analyzed, including choroidal thickness evaluation. Results. Significant macular thinning was observed in all ETDRS areas (p < 0.001) in MS patients. Peripapillary retinal, RNFL, and GCL ++ thickness showed a significant reduction in patients in all sectors (p < 0.001) except in the nasal quadrant/sector (p > 0.05). GCL+ measurements were found to be reduced in the nasal (p = 0.003), inferonasal (p = 0.045), and temporal (p = 0.001) sectors and total thickness (p < 0.001). Choroidal thickness was reduced in the outer macular ring in MS patients compared with controls (p = 0.038). Conclusion. New swept-source technology for OCT devices detects retinal thinning in MS patients, providing increased depth analysis of the choroid in these patients. MS patients present reduced retinal and choroidal thickness in the macular area and reduced peripapillary retinal, RNFL, and GCL thickness.
关键词： ganglion cell layer,choroidal thickness,retinal thickness,retinal nerve fiber layer,swept-source optical coherence tomography,multiple sclerosis
更新于2025-09-04 15:30:14
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PHOTOELECTROCHEMICAL ANALYSIS OF TITANIUM DIOXIDE BY USING OXALIC ACID AS A SACRIFICIAL DONOR
摘要： Photoelectrochemical (PEC) water splitting is a very promising green method to produce solar fuel. Titanium dioxide (TiO2) has been widely used as photocatalyst for this type of reaction. Improving the performance of TiO2 for PEC water splitting has been ongoing and addition of sacrificial donor especially from waste is an attractive option to achieve this. Oxalic acid is one component in organic waste stream that can be used as sacrificial donor. The TiO2 thin films has been fabricated by coating TiO2 paste on Fluorine Tin oxide (FTO) glass surface. The morphology of the TiO2 thin films were porous and rough with uniform particles size with crystallite size of about 20 nm and dominant anatase peak. The TiO2 photoelectrode undergo PEC testing to measure its photolectroactivity by using oxalic acid as a sacrificial donor in two different type of electrolytes which are distilled water and sodium sulfate (NA2SO4) aqueous solution. The photocurrent produced without addition of oxalic acid is much lower than with the acid. The saturation photocurrent for aqueous NA2SO4 solution and water electrolyte is 0.1 mA/cm2 and negligible respectively. While the photocurrent for addition of oxalic acid in NA2SO4 aqueous solution is 0.5 mA/cm2 and the photocurrent for oxalic acid in water only is 0.9 mA/cm2, which is almost double compared to in NA2SO4 and tenfold in water only. The highest photocurrent produced by TiO2 photoelectrode is by addition of oxalic acid in aqueous (H2O) electrolyte.
关键词： photoelectrochemical cell,titanium dioxide,donor,oxalic acid,Water splitting
更新于2025-09-04 15:30:14
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Effect of Physical Properties upon Hydrothermal Treatments on Titanium Dioxide Films
摘要： Titanium dioxide is used extensively as semiconductor since more than five decades. Hydrothermal method is one the method which widely used for the synthesis of TiO2 have been widely developed. The effect of hydrothermal temperatures on the titanium dioxide films is investigated. The TiO2 films were prepared by hydrothermally layering a mixture of aquadest, HCl and titanium(IV) isopropoxide over indium tin oxide glass, at 110, 150, 180 and 200 oC for 10 h. The film on the surface of indium tin oxide glass was calcined at 500 oC and characterized by X-ray diffraction, UV-visible spectroscopy and scanning electron microscopy-energy dispersive X-ray (SEM-EDX) methods. The study shows that the TiO2 has a spherical morphology with the size between 0.5 to 2 μm. The TiO2 consits of rutile and anatase with the particle size ranging from 16.97 to 24.10 nm and from 18.09 to 26.75 nm, respectively. The band gap energy of TiO2 is between 3.17 to 3.48 eV.
关键词： Anatase,Hydrothermal,TiO2,Solar cell
更新于2025-09-04 15:30:14
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Photoelectric Properties and Drive Characteristics of GH Liquid-Crystal Cells in Various Alignment Modes
摘要： Photoelectric properties and drive characteristics of guest-host (GH)-type liquid crystal cells with yellow, magenta, cyan, green, and gray colors using 4-cyano-4'-pentylbiphenyl (5CB) and three diazo-based dichroic dyes were systematically investigated in different driving modes. The dichroic dyes have structures similar to 5CB; therefore, they uniformly mixed and dispersed into the liquid crystal matrix. The green and gray colors were obtained by mixing appropriate ratios of two or more dyes. Impedance of the GH cells decreased upon the incorporation of the dyes into 5CB. However, the photoelectric properties and drive characteristics of the liquid crystal cells were not altered after the inclusion of the dye. In particular, the alignment of the molecules strongly depended on the cell structure. The GH cells in twisted nematic mode exhibited a low driving voltage of approximately 1.5 V.
关键词： Dichroic dyes,Guest-host cell,Liquid crystal,In-plane-switching,Twisted nematic
更新于2025-09-04 15:30:14